Preliminary investigation on cytotoxicity of fluorinated polymer nanoparticles

Wang, Xue; Cheng, Wenge; Yang, Qiuyuan; Niu, Hongyun; Liu, Qian; Liu, Yun; Gao, Ming; Xu, Ming; Xu, An; Liu, Sijin; Huang, Xiaoyu; Du, Yuguo

doi:10.1016/j.jes.2017.10.014

Cited by 20 publications

(8 citation statements)

References 35 publications

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“…At first, we considered whether ROS might oxidize lipids to cause OM damage, since ROS always damaged the membranes ( Hu et al, 2018 ; Chu et al, 2019 ). The intracellular ROS oxidized non-fluorescent DCFH to produce fluorescent DCF, which was detected to reflect the level of intracellular ROS ( Wang et al, 2018 ). The time courses of the intracellular ROS were monitored and compared ( Figure 6 ).…”

Section: Resultsmentioning

confidence: 99%

Inactivation of Salmonella typhimurium SL1344 by Chlorogenic Acid and the Impairment of Cellular Integrity

Yang

Zhang

et al. 2022

Front. Microbiol.

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Chlorogenic acid (CGA) is an antibacterial agent that can be isolated from Eucommia ulmoides Oliver, a Chinese medicinal and edible plant food. The inhibitory effect of CGA on bacterial growth and stiffness of the outer membrane (OM) had been reported, while more evidence were required to elucidate its impairment of cell wall. In this study, the morphological and physiochemical changes of Salmonella cells under CGA treatment were investigated. Firstly, the minimum inhibitory concentration (MIC) of CGA against Salmonella was assayed. Later, the permeability of OM and activity of the proteins released were measured and observed to reveal the alteration of OM characteristic and cellular morphology. Finally, reactive oxygen species and cell membrane fluidity were analyzed, respectively, to elucidate how CGA damaged cell surface. The results showed that MIC of CGA against Salmonella was 6.25 mg/L. Under sub-lethal doses of CGA, the OM permeability and the release of soluble proteins were enhanced evidently, and Salmonella cells showed more deformed and shrunken, confirming the impairment of cellular integrity under CGA. Finally, the possible cause of cell surface damage was investigated. the fluidity of the membrane was increased upon CGA treatment, which may the possible cause of OM by CGA.

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Section: Resultsmentioning

confidence: 99%

Inactivation of Salmonella typhimurium SL1344 by Chlorogenic Acid and the Impairment of Cellular Integrity

Yang

Zhang

et al. 2022

Front. Microbiol.

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“…The effects of NM exposure on the cell cycle were also investigated. Data showed that in NM-treated cells compared with negative control, the relative % hepatocytes in G0/G1 phase decreased (4.3-6.8%); while cells in the G2/M phase rose (3.3-6.7%), suggesting that the mitotic process was blocked and the cell cycle arrested (Wang et al 2018a).…”

Section: Hepatocyte Only Test Modelsmentioning

confidence: 97%

“…In vitro exposure of HepG2 cells to a collagenbased ZnO NMs (approximately 50 nm) for 24 hr showed NMs to be cytotoxic with an inhibitory concentration 50 (IC 50 ) of 42 µg/ml (Vijayakumar and Vaseeharan 2018). Wang et al (2018a) incubated HepG2 cells with three different trifluoroethyl aryl ether-based fluorinated poly (methyl methacrylate) NMs with 5%, 6.1% or 12% fluorine content (all around 600 nm) for a period of 24 hr. All NMs produced cytotoxicity above100 µg/ml irrespective of fluorine content.…”

Section: Hepatocyte Only Test Modelsmentioning

confidence: 99%

A review of hepatic nanotoxicology – summation of recent findings and considerations for the next generation of study designs

Kermanizadeh

Powell

Stone

2020

Journal of Toxicology and Environmental Health, Part B

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The liver is one of the most important multi-functional organs in the human body. Amongst various crucial functions, it is the main detoxification center and predominantly implicated in the clearance of xenobiotics potentially including particulates that reach this organ. It is now well established that a significant quantity of injected, ingested or inhaled nanomaterials (NMs) translocate from primary exposure sites and accumulate in liver. This review aimed to summarize and discuss the progress made in the field of hepatic nanotoxicology, and crucially highlight knowledge gaps that still exist. Key considerations include The review offers a number of important suggestions for the future of hepatic nanotoxicology study design. This is of great significance as its findings are highly relevant due to the development of more advanced in vitro, and in silico models aiming to improve physiologically relevant toxicological testing strategies and bridging the gap between in vitro and in vivo experimentation.

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“…Fluorine was widely used in aerospace, petroleum, chemical, and other areas. It flows into the water cycle through wastewater discharge, and it cannot be metabolized and is enriched in biological extraction, causing great harm [12]. The reported siloxane thickeners [13][14][15][16][17] rely on the formation of Lewis acid hydrogen bonds between the cosolvent and CO 2 and the similar compatibility of the cosolvent with siloxane to enhance solubility.…”

Section: Materials Studio Simulationmentioning

confidence: 99%

Preparation and Performance of Supercritical Carbon Dioxide Thickener

Liu¹,

Wang²,

Lei³

2020

Polymers

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The low sand-carrying problem caused by the low viscosity of supercritical carbon dioxide (SC–CO2) limits the development of supercritical CO2 fracturing technology. In this study, a molecular simulation method was used to design a fluorine-free solvent-free SC–CO2 thickener 1,3,5,7-tetramethylcyclotetrasiloxane (HBD). Simulations and experiments mutually confirm that HBD-1 and HBD-2 have excellent solubility in SC–CO2. The apparent viscosity of SC–CO2 after thickening was evaluated with a self-designed and assembled capillary viscometer. The results show that when the concentration of HBD-2 is 5 wt.% (305.15 K, 10 MPa), the viscosity of SC–CO2 increases to 4.48 mPa·s. Combined with the capillary viscometer and core displacement device, the low damage of SC–CO2 fracturing fluid to the formation was studied. This work solves the pollution problems of fluoropolymers and co-solvents to organisms and the environment and provides new ideas for the molecular design and research of SC–CO2 thickeners.

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Preliminary investigation on cytotoxicity of fluorinated polymer nanoparticles

Cited by 20 publications

References 35 publications

Inactivation of Salmonella typhimurium SL1344 by Chlorogenic Acid and the Impairment of Cellular Integrity

Inactivation of Salmonella typhimurium SL1344 by Chlorogenic Acid and the Impairment of Cellular Integrity

A review of hepatic nanotoxicology – summation of recent findings and considerations for the next generation of study designs

Preparation and Performance of Supercritical Carbon Dioxide Thickener

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