Preliminary results from the Scandinavian multicentre evaluation of in vitro cytotoxicity (MEIC)

Canine progenitor epidermal keratinocytes express various inflammatory markers, including interleukin‐8 and CD40, which are affected by certain antibiotics

“…Other studies have found cytotoxic effects in primary and immortalized cells with similar high concentrations of enrofloxacin . Importantly, it is not uncommon for toxicologists to need to use higher doses in vitro to obtain an effect that would be seen at lower concentrations in vivo , especially when using immortalized cells . This mirrors the fact that LD50s determined in laboratory dogs are often much higher than doses that induce significant toxicity in the general canine population.…”

“…[20][21][22] Importantly, it is not uncommon for toxicologists to need to use higher doses in vitro to obtain an effect that would be seen at lower concentrations in vivo, especially when using immortalized cells. 86,87 This mirrors the fact that LD50s determined in laboratory dogs are often much higher than doses that induce significant toxicity in the general canine population. Enrofloxacin 500 lmol/L is higher than what has been reported in the plasma (range 0.5-30 lmol/L).…”

In Vitro biological toxicity assessments for fire combustion products

“…40 From this concept a new classification of chemical toxicity into three categories was developed; extracellular toxicity, organ-specific toxicity and basal cytotoxicity. The basal cytotoxicity concept is based on the fact that chemicals are toxic to humans by interfering with cell functions common to all human cells (cyto cells).…”

3.3. Eye Irritation

“…However, larger-scale validation is required, since the PTG assay has not been assessed in parallel by more than two or three laboratories in any of the studies mentioned. The PTG assay was included in the Multicenter Evaluation of In Vitro Cytotoxicity (MEIC) Programme of the Scandinavian Society of Cell Toxicology (191,192). Key references.…”