Preliminary separation and purification of rutin and quercetin from Euonymus alatus (Thunb.) Siebold extracts by macroporous resins

Zhao, Zhan-Yi; Dong, Lu-Lu; Wu, Yanli; Lin, Feng

doi:10.1016/j.fbp.2010.11.001

Cited by 70 publications

(28 citation statements)

References 16 publications

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“…In recent years, macroporous adsorption resin is more and more used in the purification of pigments [19,20]. Especially, AB-8 resin is a kind of macroporous resin for the purification of flavonoid [21].…”

Section: Introductionmentioning

confidence: 99%

Extraction and Purification of Anthocyanins from the Fruit Residues of Vaccinium uliginosum Linn

Zhang¹

2013

J Chromat Separation Techniq

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An aqueous two-phase system was developed to extract anthocyanins present in the fruit residue during juice production from the fruit of Vaccinium uliginosum Linn. A maximum partition coefficient of 10.67 and a recovery of 96.09% for anthocyanins could be obtained using an extraction system consisted of 30% (w/w) ethanol and 19% ammonium sulfate. Compared with the traditional extraction using acidified ethanol, the novel aqueous two-phase extraction could not only yield a much higher concentration of anthocyanins, save more ethanol, energy, and time, but also decrease impurities in extract, e.g. proteins and sugars by 58% and 66%, respectively. AB-8 macroporous resin was applied to the purification of anthocyanins. A novel and simple separation technique for anthocyanins was developed by integrating aqueous two-phase extraction and macroporous resin column chromatography. This new technology might be a suitable for other bioactive natural products on industrial scale.

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Section: Introductionmentioning

confidence: 99%

Extraction and Purification of Anthocyanins from the Fruit Residues of Vaccinium uliginosum Linn

Zhang¹

2013

J Chromat Separation Techniq

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“…As shown in Figure 2(b), the desorption ratios of alkaloids increased with the increase of ethanol concentration and reached their peak value at the concentration of 80%, then decreased with the increase of ethanol concentration [23]. …”

Section: Resultsmentioning

confidence: 99%

“…All the solutions after adsorption and desorption at different conditions were analyzed by UV-Vis spectrophotometer [23]. …”

Section: Methodsmentioning

confidence: 99%

Optimization of Extraction and Enrichment of Steroidal Alkaloids from Bulbs of CultivatedFritillaria cirrhosa

Wang

et al. 2014

BioMed Research International

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The bulbs of cultivated Fritillaria cirrhosa (BCFC) are used in China both for food and folk medicine due to its powerful biological activities. The aim of this study is to optimize the extraction and enrichment conditions of alkaloids from BCFC. Firstly, the orthogonal experimental design was used to optimize and evaluate four variables (ethanol concentration, solid-liquid ratio, extraction time, and temperature). Thereafter, resin adsorption was as a means to enrich alkaloids. Among 16 tested resins, H-103 resin presented higher adsorption capacity and desorption ratio. The equilibrium experimental data of the adsorption of total alkaloids, imperialine, and peimisine were well-fitted to the pseudo-first-order kinetics model, Langmuir and Freundlich isotherms models. Finally, in order to optimize the parameters for purifying alkaloids, dynamic adsorption and desorption tests were carried out. After one run treatment with H-103 resin, the contents of total alkaloids, imperialine, and peimisine in the product were 21.40-, 18.31-, and 22.88-fold increased with recovery yields of 94.43%, 90.57%, and 96.16%, respectively.

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“…euoverrine and eunonymol), polyamines, sesquiterpenes, and proteins (lectin) [4][5][6]. A few scientific papers confirm the presence of flavonoids in different species of the Euonymus gender [4,7].…”

Section: Introductionmentioning

confidence: 99%

“…Based on recent scientific papers on the flavonoid content of Euonymus spp., the proposed results draw attention to another group of compounds with radical-scavenging properties -phenolic acids [4,7]. Phenolic substances (simple phenols, phenolic acids, flavonoids, or hydroxycinnamic acid derivatives) belong to the group of nutrients characterized by strong antioxidant capacity [13,14].…”

Section: Introductionmentioning

confidence: 99%

HPLC, Two-Dimensional TLC Determination of Phenolic Content, and anIn VitroPerspective to Antioxidant Potential ofEuonymus verrucosusScop. Extracts

Kukuła-Koch

Widelski

Koch

et al. 2015

Acta Chromatographica

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Summary.The presence of phenolic content in overground extracts of Euonymus verrucosus Scop. -commonly growing in Europe -has been reported recently. The chromatographical and spectral data revealed the presence of several simple phenolic acids (gallic, protocatechuic, p-hydroxybenzoic, vanillic, syringic, caffeic, p-coumaric, ferulic, and m-coumaric acids), both as free and conjugated with other secondary metabolites. The comparison of two-dimensional TLC systems on cellulose stationary phases with HPLC-DAD reversed-phase chromatography was performed to assess a cheap and rapid technique in the identification process of major phenolic constituents. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging tests, expressed as IC 50 , revealed the most beneficial results for the fraction after alkaline hydrolysis and yielded 205 ± 8 μg mL −1 .

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Preliminary separation and purification of rutin and quercetin from Euonymus alatus (Thunb.) Siebold extracts by macroporous resins

Cited by 70 publications

References 16 publications

Extraction and Purification of Anthocyanins from the Fruit Residues of Vaccinium uliginosum Linn

Extraction and Purification of Anthocyanins from the Fruit Residues of Vaccinium uliginosum Linn

Optimization of Extraction and Enrichment of Steroidal Alkaloids from Bulbs of CultivatedFritillaria cirrhosa

HPLC, Two-Dimensional TLC Determination of Phenolic Content, and anIn VitroPerspective to Antioxidant Potential ofEuonymus verrucosusScop. Extracts

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