Preliminary studies on induction of apoptosis by abamectin in Spodoptera frugiperda (Sf9) cell line

Cytotoxic and genotoxic effects of abamectin, chlorfenapyr, and imidacloprid on CHOK1 cells

“…Our results indicate that abamectin cytotoxicity was time-dependent, particularly in the MTT assay (p<0.001). In agreement with our findings, Huang et al (2011) reported that abamectin decreased the cell viability in a time-dependent manner in Sf9 cell line. Chlorfenapyr exhibited cytotoxic effects in the three assays.…”

“…Maioli et al (2013) proved that abamectin, not its metabolites, is responsible for the cytotoxic effect on isolated hepatocytes. The ultrastructural examination showed swollen mitochondria and unclear structure of the inner membranes in abamectintreated cells from Spodoptera frugiperda (Sf9) (Huang et al 2011). The authors supposed that mitochondria may be involved in the apoptosis initiation effect of abamectin.…”

Synergistic effect of non-ionic surfactants Tween 80 and PEG6000 on cytotoxicity of insecticides

“…Our experiments showed that avermectin exhibited strong cytotoxicity on Tn5B1-4 and HepG2 cells, this is consistent with the report of Huang et al (2011) that abamectin could suppress cell growth and induce cell apoptosis of Spodoptera frugiperda Sf-9 cells. Median cytotoxicity of chlorfluazuron and hexaflumuron on Tn5B1-4 and HepG2 cells was observed, and chlorpyrifos and tebufenozide even at concentration of 20 M did not exhibit significant cytotoxicity.…”

The cytotoxicology of momordicins I and II on Spodoptera litura cultured cell line SL-1

“…These changes are similar to the morphological changes induced by azadirachtin [32,33]. Azadirachtin A caused morphological changes in Sf9 cells by suppression of tubulin polymerization [18,34]. Several studies have also reported the destruction of actin after exposure of Drosophila compound eye cells to azadirachtin A [24,35,36].…”

“…Then 200 μL PI solution was added and cells were incubated for 30 min at 4°C in darkness. Incubated cells were processed in FCM using the methods of Huang et al [18,19]. At least 2.0 × 10 4 cells were used in each assay.…”