Preparation and application of organic‐silica hybrid monolithic capillary columns

Abstract

Organic-silica hybrid monolithic columns have drawn more and more attention due to the ease of preparation and good mechanical stability in recent years. Many synthetic approaches have been developed and a variety of hybrid monolithic capillary columns have been prepared. The sol-gel process is well recognized in the fabrication of hybrid monolithic columns, which can be mainly classified as one-step, acid/base two-step procedures. The new approaches such as the ''one-pot'' and nano-scaled inorganicorganic hybrid reagent of polyhedral oligomeric silsesquioxane used as a cross-linker have also emerged for the preparation of hybrid monolithic columns. The applications of the organic-silica hybrid monolithic capillary columns for capillary electrochromatography, micro high-performance liquid chromatography, solid-phase microextraction and enzymatic reactor etc. are included in this review.

Polyhedral oligomeric silsesquioxanes as functional monomer to prepare hybrid monolithic columns for capillary electrochromatography and capillary liquid chromatography

“…The approach to prepare organic-silica hybrid monoliths generally makes use of sol-gel chemistry [39]. Meanwhile, an inorganic-organic hybrid monolithic column was simply fabricated by using a POSS reagent containing several methacrylate groups as crosslinker via free radical polymerization [36].…”

Hybrid monolithic columns coated with cellulose tris(3,5-dimethylphenyl-carbamate) for enantioseparations in capillary electrochromatography and capillary liquid chromatography

“…Many hybrid monoliths have been used as stationary phases for CLC or CEC separation, as well as for sample pretreatment [34][35][36]. To the best of our knowledge, however, few hybrid monoliths were applied for preparation of CSPs.…”

Recent advances in monolithic columns for protein and peptide separation by capillary liquid chromatography

“…Nevertheless, the complicated and difficult preparation process, nonspecific adsorption caused by Si-OH, and a narrow pH working range limit their applications. Organic-silica hybrid monoliths have the combined advantages of the two abovementioned types, including ease of preparation, good mechanical stability, good pH stability, and high surface area [17,18], and thus have attracted great attention in recent years.…”