Preparation and Characterization of a Composite of Demineralized Bone Matrix Fragments and Polylactide Beads for Bone Tissue Engineering

Thomas, Chuck B.; Maxson, Scott; Burg, Karen J. L.

doi:10.1163/092050610x488232

Cited by 10 publications

(7 citation statements)

References 21 publications

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“…ASCs have been used together with acellular matrices from different sources. Allografts and xenografts have been derived mainly from bone tissue [55, 58, 60, 76, 77] but also from small intestine submucosa [57] or from adipose tissue [59].…”

Section: Scaffolds and Adipose Stem Cellsmentioning

confidence: 99%

Adipose-Derived Stem Cells in Bone Tissue Engineering: Useful Tools with New Applications

Storti

Scioli

Kim

et al. 2019

Stem Cells International

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Adipose stem cells (ASCs) are a crucial element in bone tissue engineering (BTE). They are easy to harvest and isolate, and they are available in significative quantities, thus offering a feasible and valid alternative to other sources of mesenchymal stem cells (MSCs), like bone marrow. Together with an advantageous proliferative and differentiative profile, they also offer a high paracrine activity through the secretion of several bioactive molecules (such as growth factors and miRNAs) via a sustained exosomal release which can exert efficient conditioning on the surrounding microenvironment. BTE relies on three key elements: (1) scaffold, (2) osteoprogenitor cells, and (3) bioactive factors. These elements have been thoroughly investigated over the years. The use of ASCs has offered significative new advancements in the efficacy of each of these elements. Notably, the phenotypic study of ASCs allowed discovering cell subpopulations, which have enhanced osteogenic and vasculogenic capacity. ASCs favored a better vascularization and integration of the scaffolds, while improvements in scaffolds' materials and design tried to exploit the osteogenic features of ASCs, thus reducing the need for external bioactive factors. At the same time, ASCs proved to be an incredible source of bioactive, proosteogenic factors that are released through their abundant exosome secretion. ASC exosomes can exert significant paracrine effects in the surroundings, even in the absence of the primary cells. These paracrine signals recruit progenitor cells from the host tissues and enhance regeneration. In this review, we will focus on the recent discoveries which have involved the use of ASCs in BTE. In particular, we are going to analyze the different ASCs' subpopulations, the interaction between ASCs and scaffolds, and the bioactive factors which are secreted by ASCs or can induce their osteogenic commitment. All these advancements are ultimately intended for a faster translational and clinical application of BTE.

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Section: Scaffolds and Adipose Stem Cellsmentioning

confidence: 99%

Adipose-Derived Stem Cells in Bone Tissue Engineering: Useful Tools with New Applications

Storti

Scioli

Kim

et al. 2019

Stem Cells International

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“…160,161 To take advantage of this property, decellularized tissues can be powdered and mixed with synthetic polymeric materials during scaffold formation to confer tissue-specific bioactivity to synthetic scaffolds. This hybrid approach employs a combination of the top-down (decellularized tissue) and bottom-up (incorporating a bioactive component into a synthetic scaffold) approaches for the modification of synthetic scaffolds.…”

Section: Hybrid Approaches For Bte Scaffold Modificationmentioning

confidence: 99%

Approaches for building bioactive elements into synthetic scaffolds for bone tissue engineering

Kesireddy

Kasper

2016

J. Mater. Chem. B

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Bone tissue engineering (BTE) is emerging as a possible solution for regeneration of bone in a number of applications. For effective utilization, BTE scaffolds often need modifications to impart biological cues that drive diverse cellular functions such as adhesion, migration, survival, proliferation, differentiation, and biomineralization. This review provides an outline of various approaches for building bioactive elements into synthetic scaffolds for BTE and classifies them broadly under two distinct schemes; namely, the top-down approach and the bottom-up approach. Synthetic and natural routes for top-down approaches to production of bioactive constructs for BTE, such as generation of scaffold-extracellular matrix (ECM) hybrid constructs or decellularized and demineralized scaffolds, are provided. Similarly, traditional scaffold-based bottom-up approaches, including growth factor immobilization or peptide-tethered scaffolds, are provided. Finally, a brief overview of emerging bottom-up approaches for generating biologically active constructs for BTE is given. A discussion of the key areas for further investigation, challenges, and opportunities is also presented.

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“…Although DBM is weakened during the demineralization process, several reports have shown that the inclusion of synthetic polymers with DBM resulted in increased construct mechanical integrity. 56,69,118 Blends of natural and synthetic polymers have also been electrospun to create a bioactive scaffold with enhanced mechanical properties. GAG/collagen electrospun materials were used to facilitate dermal regeneration.…”

Section: Structural Propertiesmentioning

confidence: 99%

Functional Augmentation of Naturally-Derived Materials for Tissue Regeneration

Allen

Priddy

et al. 2014

Ann Biomed Eng

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Tissue engineering strategies have utilized a wide spectrum of synthetic and naturally-derived scaffold materials. Synthetic scaffolds are better defined and offer the ability to precisely and reproducibly control their properties, while naturally-derived scaffolds typically have inherent biological and structural properties that may facilitate tissue growth and remodeling. More recently, efforts to design optimized biomaterial scaffolds have blurred the line between these two approaches. Naturally-derived scaffolds can be engineered through the manipulation of intrinsic properties of the pre-existing backbone (e.g., structural properties), as well as the addition of controllable functional components (e.g., biological properties). Chemical and physical processing techniques used to modify structural properties of synthetic scaffolds have been tailored and applied to naturally-derived materials. Such strategies include manipulation of mechanical properties, degradation, and porosity. Furthermore, bio-functional augmentation of natural scaffolds via incorporation of exogenous cells, proteins, peptides, or genes has been shown to enhance functional regeneration over endogenous response to the material itself. Moving forward, the regenerative mode of action of naturally-derived materials requires additional investigation. Elucidating such mechanisms will allow for the determination of critical design parameters to further enhance efficacy and capitalize on the full potential of naturally-derived scaffolds.

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Preparation and Characterization of a Composite of Demineralized Bone Matrix Fragments and Polylactide Beads for Bone Tissue Engineering

Cited by 10 publications

References 21 publications

Adipose-Derived Stem Cells in Bone Tissue Engineering: Useful Tools with New Applications

Adipose-Derived Stem Cells in Bone Tissue Engineering: Useful Tools with New Applications

Approaches for building bioactive elements into synthetic scaffolds for bone tissue engineering

Functional Augmentation of Naturally-Derived Materials for Tissue Regeneration

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