Preparation and characterization of enzymatically cross-linked gelatin/cellulose nanocrystal composite hydrogels

Abstract

Gelatin is an attractive hydrogel material because of its excellent biocompatibility and non-cytotoxicity, but poor mechanical properties of gelatin-based hydrogels become a big obstacle that limits their wide-spread application.

The Preparation and Properties of Composite Hydrogels Based on Gelatin and (3-Aminopropyl) Trimethoxysilane Grafted Cellulose Nanocrystals Covalently Linked with Microbial Transglutaminase

“…It can be found that all the gelatin-based hydrogels formed a homogenous three-dimensional network. As reported in our previous study [ 25 ], this structure was attributed to the supporting effect of CNCs on the gelatin matrix. All hydrogels presented a cellular structure with smooth walls, and no CNCs can be clearly identified in the pore wall of the scaffold.…”

“…The hydrogels exhibited thermo-reversible behavior and a general trend was that G′ declined with the increase in temperature. In the presence of mTG, chemical-physical gels were formed, where the physical networks (self-assembly triple helix, hydrogen bonding and hydrophobic linkages) have cooperated with covalent cross-linking [ 25 , 31 ] to maintain the stability of the hydrogel. With the increasing temperature, the collagen triple helix unfolding was activated and other physical cross-linking interactions became progressively weaker.…”

“…Gel strength was the inherent structural feature of gelatin-base hydrogels for predicting their physical characteristic. In the previous work, we found that the gel strength had been significantly improved by adding CNCs and mTG into gelatin [ 25 ]. In this study, we used APTMS to modify CNCs to introduce amino groups on its surface to form a covalent cross-link between the gelatin chain and CNCs.…”

“…In our previous study, CNCs were used as a nanofiller to prepare gelatin/CNCs composite hydrogels (Gel-TG-CNCs) by the enzymatic cross-linking method. The as-prepared Gel-TG-CNCs proved to have excellent biocompatibility and improved mechanical properties [ 25 ]. However, only physical interaction between gelatin chain and CNCs was formed in Gel-TG-CNCs.…”

Engineering of hybrid anticancer drug-loaded polymeric nanoparticles delivery system for the treatment and care of lung cancer therapy

“…Owing to the antitumor drugs' different physicochemical properties, encapsulating multiple anticancer medicines in a single nanoparticle has proved difficult (Yu et al, 2014 ; Tu et al, 2017 ; Rao et al, 2018 ; Xu et al, 2018 ). Bio-degradable nanoparticles, which are created by en5FUulating a variety of medications with discrete physico-chemical behaviors and controlling their controlled releasing in regulated proportions, are thus thought to be more effective at delivering drugs to cancer cells (Lee & Nguyen, 2013 ; Li et al, 2014 ; Dong et al, 2021 ). Hybrid anticancer delivery may be used to achieve combination treatment with a sensitizer (Mao et al, 2021 ; Pragya et al, 2021 ; Tran et al, 2021 ).…”