Preparation and Characterization of Gold Nanoparticles in the Presence of Citrate and Soybean Seed Extract in an Acidic Conditions

Abstract

Through the present study, an eco-friendly method was used to synthesize the gold nanoparticles (GNPs) by using the sodium citrate and extract of the soybean seed as reducing the agents at PH 3. X-Ray diffraction (XRD) method was used to evaluate the crystal structure of as-synthesized NPs and it's revealed that this method leads to well crystallized GNPs. In order to determine the particle size and their distribution, field emission scanning microscopy (FE-SEM) and dynamic light scattering (DLS) were used. The results showed that, the average particle size distribution of synthesized GNPs in solutions containing of the soybean extract and 1% citrate at PH 3 is about 109.6 and 140.9 nm, respectively. Also, we find that the average size of GNPs is 40 and 33 nm from solutions of citrate and soybean extract, respectively. It was concluded that using the extract of soybean seeds as reducing agent can lead to GNPs with small size and narrow size distribution.

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“…Sodium citrate is a reducing agent for the synthesis of metal nanoparticles, such as gold, silver, copper, and so on. 30 Recently, Gutieŕrez-Serpa et al 31 synthesized gold nanoparticles (AuNPs) by three different reagents, sodium citrate, chloroauric acid, and hydrogen peroxide, and demonstrated that the size of AuNPs was 20−100 nm through measuring the UV spectrum. In this investigation, 100 mL of 0.25 mM chloroauric acid was added into 250 mL volumetric flask and then stirred.…”