Preparation and Characterization of Insoluble Silk Fibroin/Chitosan Blend Films

Moraes, Mariana Agostini de; Nogueira, Grínia M.; Weska, Raquel Farias; Beppu, Marisa Masumi

doi:10.3390/polym2040719

Cited by 87 publications

(54 citation statements)

References 20 publications

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“…SF can assume distinct conformations; α-helix and random-coil conformations (also called silk I) and the β-sheet conformation (silk II). Silk I is a metastable form that is soluble in water and silk II is insoluble in water and is the most stable structure, where SF chains are connected by hydrogen bonds between the adjacent segments of polypeptide chains 5 , 15 - 17 …”

Section: Introductionmentioning

confidence: 99%

The role of dialysis and freezing on structural conformation, thermal properties and morphology of silk fibroin hydrogels

et al. 2014

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Silk fibroin has been widely explored for many biomedical applications, due to its biocompatibility and biodegradability. The aim of this work was to study the role of dialysis and freezing on structural conformation, thermal properties and morphology of silk fibroin hydrogels. Hydrogels were prepared after 3 and 7 days of dialysis and the effect of freezing was analyzed. For that purpose, a part of the fibroin hydrogels underwent freezing at -20 °C for 24 h, followed by lyophilization and the rest of the hydrogels were kept at 8 °C for 24 h, with further lyophilization. The fibroin hydrogels were characterized by X-ray diffraction (XRD), Fourier transformed infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM). Measurements by XRD and FTIR indicated that silk I and silk II structures were present in the fibroin hydrogels and that the secondary structure of fibroin is transformed mostly to β-sheet during the gelation process. Thermal analysis indicated that fibroin hydrogels are thermally stable with the degradation peak at around 330–340 °C. SEM micrographs showed porous structures and the fibroin hydrogels subjected to freezing presented a much larger pore size. Results indicate that the dialysis time and freezing did not alter the material crystallinity, conformation or thermal behavior; however, hydrogel microstructure was strongly affected by dialysis time and freezing, showing controlled pores size. This study provides fundamental knowledge on silk fibroin hydrogels preparation and properties and the studied hydrogels are promising to be used in the biomaterial field.

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Section: Introductionmentioning

confidence: 99%

The role of dialysis and freezing on structural conformation, thermal properties and morphology of silk fibroin hydrogels

et al. 2014

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“…Subsequently, we washed the SF extract with distilled water and dissolved it in a solution of CaCl 2 , H 2 O and CH 3 CH 2 OH 1:8:2 molar between 45 °C and 55 °C for one hour [19][20][21]. The solution underwent a dialysis process, with distilled water in cellulose acetate membranes for five days to remove the inorganic salts remaining from the previous processes.…”

Section: B Fibroin (Sf) Preparation and Characterizationmentioning

confidence: 99%

Degradation, water uptake, injectability and mechanical strength of injectable bone substitutes composed of silk fibroin and hydroxyapatite nanorods

Buitrago-Vásquez

Orozco

2018

Rev. Fac. Ing.

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Injectable bone substitutes are generally composite materials capable of being extruded through a device; they consist of a solid phase inside a matrix that allows the easy movement of particles. Injectable bone substitutes have the advantage of allowing its application in situ without the need of invasive surgical techniques, guaranteeing a good recovery; for this reason, they are a promising alternative to replace conventional techniques to repair bone defects. Conventional techniques include the use of allografts and autografts, which often cause adverse reactions, and are disadvantageous for both the patient and the doctor. Furthermore, there are no reports regarding bone substitute development in Colombia, creating the necessity to research composite materials that could become injectable bone substitutes. In this study, we manufactured injectable bone substitutes with hydroxyapatite and calcium phosphate, which is most similar to bone tissue, and synthesized them in nanorods with shape and size similar to the natural hydroxyapatite found inside the body. Additionally, we used extracted silk fibroin from silkworm cocoons of Bombyx mori, a natural polymer of protein nature with high mechanical properties and excellent biocompatibility. For the materials manufactured, we evaluated degradation, in a simulated body fluid (SBF) at normal body temperature, water uptake, injectability and mechanical strength. The manufactured bone substitutes showed good degradation and water uptake properties, an approximate 97% injectability, and low mechanical resistance, indicating promising properties to be used as an injectable bone substitute.

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“…Therefore, according to thermal degradation temperature ( Figure 8) the randomly distributed SF was used in this work. 24 .…”

Section: 5-tensile Testingmentioning

confidence: 99%

Characterization of Membrane of Poly (L,co-D,L-lactic acid-co-trimethylene carbonate) (PLDLA-co-TMC) (50/50) loaded with Silk Fibroin

Chaud¹,

Komatsu²,

Aranha³

et al. 2017

SDRP-JBE

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Silk produced by Bombyx-mori consists of two proteins sericin and fibroin. Silk Fibroin (SF) an attractive material for epithelial tissue regeneration. The aim of this study was to characterize membranes of PLDLAco-TMC:SF (2:1 w/w) once it could be used as a wound dressing to burn treatment and epithelial tissue regeneration. Membranes of Poly(L-co-D,L-lactic acid-co-TMC) with SF were prepared by casting method and it was analysed by: FTIR -there was no change in the PLDLA-co-TMC spectrum after SF has been added in polymers' solution, Swelling Test -the samples with SF had a different swelling behaviour of PLDLA-co-TMC. Membrane with SF showed swelling rate faster than PLDLA-co-TMC. Contact angle measurement -PLDLAco-TMC (1:1 w/w) showed a contact angle value higher than samples with SF. SEM -the addition of SF in PLDLA-co-TMC apparently leaves the polymer surface more roughened. Tensile test -there was a decrease in the Young's modulus and there was an increase in the elongation at break of PLDLA-co-TMC:SF membrane, DSC -SF did not change the glass temperature (Tg) of PLDLAco-TMC. The membrane of PLDLA-co-TMC:SF was characterized and it has potential to be used as a wound dressing to burn treatment and epithelial tissue regeneration.

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Preparation and Characterization of Insoluble Silk Fibroin/Chitosan Blend Films

Cited by 87 publications

References 20 publications

The role of dialysis and freezing on structural conformation, thermal properties and morphology of silk fibroin hydrogels

The role of dialysis and freezing on structural conformation, thermal properties and morphology of silk fibroin hydrogels

Degradation, water uptake, injectability and mechanical strength of injectable bone substitutes composed of silk fibroin and hydroxyapatite nanorods

Characterization of Membrane of Poly (L,co-D,L-lactic acid-co-trimethylene carbonate) (PLDLA-co-TMC) (50/50) loaded with Silk Fibroin

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