Preparation and characterization of macroporous monoliths imprinted with erythromycin

Abstract

The synthesis of macroporous molecularly imprinted monoliths was performed using the monomers system 2-hydroxyethyl methacrylate-ethylene glycol dimethacrylate and erythromycin as a template. The copolymerization was carried out in situ inside 50 mm × 4.6 mm i.d. stainless-steel tubing. The morphology of the monoliths was examined with scanning electron microscopy. The porous characteristics were determined both from the data of hydrodynamic permeability of monoliths and by means of mercury intrusion porosimetry. The retention parameters of target substance (erythromycin), values of calculated imprinting factors and apparent dynamic dissociation constants were obtained for monoliths prepared with the application of different amount of template (4, 8 and 12 mol%). The separations of the mixtures azithromycin/erythromycin and ciprofloxacin/erythromycin were demonstrated. Additionally, the possibility of erythromycin quantification in human blood plasma was shown.

Determination of macrolide antibiotics residues in pork using molecularly imprinted dispersive solid‐phase extraction coupled with LC–MS/MS

“…Therefore, a 2:3 ratio of chloroform to acetonitrile was chosen. Furthermore, the effect of different porogen volumes (10,15,20,25,30, and 35 mL) on adsorption was investigated ( Figure 2B). The adsorption capacity of MIP initially increases with the increasing porogen volume, but it gradually decreases with porogen volumes higher than 20 mL.…”

“…Moreover, 1% acetic acid in acetonitrile is the optimal extraction solvent, with the recoveries of more than 80% for all target analytes. Then the extraction solution from pork sample was subjected to MIDSPE procedure, and different adsorbent amounts (10,15,20, and 30 mg) were investigated in this study. The results show that the recoveries of MALs increase with the rising of adsorbent amounts.…”

Preparation of magnetic glycoprotein‐imprinted nanoparticles with dendritic polyethyleneimine as a monomer for the specific recognition of ovalbumin from egg white

“…Molecularly imprinted polymers (MIPs) with selective binding sites possess the ability of recognizing target molecules. They are prepared by co‐polymerization of functional and cross‐linking monomers in the presence of the target molecules, and then removing the target molecules . The binding cavities of the MIPs are complementary in shape, size, and functionality with respect to the target molecules, thus leading to specifically identification the target molecules.…”

Molecular Imprinting in Separation Science

“…These papers cover a variety of topics, all related to the preparation of and/or applications of MIPs in separation science. A group of 15 papers mainly contains novel synthetic procedures and characterization of the products but includes applications as well. Fibers and membranes have been made by electrospinning , different polymerization techniques including hierarchical imprinting and Pickering emulsion imprinting , distillation precipitation polymerization , and atom transfer radical polymerization .…”

“…Fibers and membranes have been made by electrospinning , different polymerization techniques including hierarchical imprinting and Pickering emulsion imprinting , distillation precipitation polymerization , and atom transfer radical polymerization . The properties of magnetic polymers were studied , and the preparation and binding characteristics of several polymers have been included . A group of eight papers is mainly concerned with environmental issues, including analyses of water , food and other samples .…”