Preparation and characterization of molecularly‐imprinted polymers for extraction of sanshool acid amide compounds followed by their separation from pepper oil resin derived from Chinese prickly ash (Zanthoxylum bungeanum)

Abstract

Molecularly imprinted polymers were prepared using the molecular structure analogs of sanshool as template molecule, 2-vinylpyridine and β-cyclodextrin as double functional monomers, ethylene dimethacrylate as cross linker, and azobisisobutyronitrile as initiator. The structural characteristics of the polymers were determined by Fourier-transform infrared spectroscopy and scanning electron microscopy. Dynamic adsorption and isothermal adsorption were also investigated. The molecularly imprinted polymers were used to prepare a molecularly imprinted solid-phase extraction column in order to separate acid amide components from pepper oil resin derived from Chinese prickly ash (Zanthoxylum bungeanum). After eluting, the percentage of acid amide components was enhanced to 92.40 ± 1.41% compared with 23.34 ± 1.21% in the initial pepper oil resin, indicating good properties of purification of molecularly imprinted polymers and potential industrial application.

Recent Advances of Molecularly Imprinted Polymers Based on Cyclodextrin

“…Chen et al. [ 21 ] reported a SPEC based on a MIP with methylacryloyl‐β‐CD and 2‐VP as bi‐functional monomers to separate acid amide components from pepper oil resin derived from Chinese prickly ash. The MIP exhibited selective capability of the target components with a saturated concentration of 45.04 μg mg −1 .…”

Isolation of sinapic acid from broccoli using molecularly imprinted polymers

“…Then, when 60:40 v/v ratio of methanol/water was used, the IF of MIP 3 was >20 in comparison to MIP 5 having an IF of 1.117 which indicated that MIP 3 had a better binding behavior. These results were expected due to the basic nature of 4-VP, which enhanced its binding to acidic templates [7]. Also, it was found from previous studies that 4-VP could be successfully used as a functional monomer for other phenolic acids as chicoric acid [10], caffeic acid [18], cinnamic acid, and ferulic acid [25,26].…”

“…They are able to bind selectively to their target molecule while it is present among structural analogues of similar size and structure. MIPs have numerous advantages, which made them of high interest to researchers nowadays . They have widely been used in purification, separation, and are promising candidates for drug delivery applications .…”

Identification of the candidate genes related to sanshool biosynthesis in Zanthoxylum bungeanum by comparative RNA sequencing analysis

“…Among these metabolites, aliphatic amides, which are classified as sanshools, are the major active components. They have a unique pungent taste and cause paresthetic sensations on the tongue 5 . Furthermore, sanshools and their analogues have been shown to induce apoptosis of human hepatocarcinoma HepG2 cells and block expression of the oncogene KCNK9 6,7 .…”