Preparation and characterization of PLGA-β-CD polymeric nanoparticles containing methotrexate and evaluation of their effects on T47D cell line

Gorjikhah, Fatemeh; Jalalian, Farid Azizi; Salehi, Roya; Panahi, Yunes; Hasanzadeh, Arash; Akbarzadeh, Abolfazl; Davaran, Soodabeh

doi:10.3109/21691401.2016.1160915

Cited by 30 publications

(20 citation statements)

References 57 publications

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“…Encapsulation with PLGA polymer Double emulsion method (water-in-oil-in-water or w/o/w) was used for synthesis of PLGA and peptide-loaded PLGA nanoparticles [11]. PLGA was dissolved in dichloromethane (DCM), and peptide in aqueous solution was added.…”

Section: Methodsmentioning

confidence: 99%

Molecular docking of immunogenic peptide ofToxoplasma gondiiand encapsulation with polymer as vaccine candidate

Çakır-Koç

Budama‐Kilinc

Kökcü

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Toxoplasma gondii is one of the most widely spread parasitic organisms in the world. T. gondii causes primary, chronic infection and mortality. Major surface antigen 1 is the most abundant tachyzoite surface protein and highly conserved between species and causes strong humoural response. Some studies showed that the peptide sequence of surface antigen has immunity. Therefore, tachyzoite surface antigenic peptide sequence is one of the good candidates for vaccine development. However, conformational information and delivery systems are very important parameters for vaccine development. Computational chemistry which is used as an effective method to perform drug or vaccine design provides important information on structure-activity relationship, biological effects of functional groups, molecular geometry, design of enzyme inhibitors and antagonists. The interaction of immunological peptides with protein systems was carried out by means of computing the free energy of binding using the molecular docking technique. Due to the major histocompatibility complex (MHC), proteins play a substantial role for adaptive immunity, the crystal structure of a MHC class I, which plays a pivotal role in the adaptive branch of the immune system, was preferred for docking calculations. A delivery system based on poly(lactic-co-glycolic acid) (PLGA) nanoparticles and peptide loaded PLGA nanoparticles was prepared in this study to improve the bioavailability of tachyzoite surface antigenic peptide sequence. Double emulsion method (water-in-oil-in-water or w/o/w) was used for synthesis of PLGA and peptide loaded PLGA nanoparticles. The average particle size, polydispersity index and zeta potential values of PLGA and peptide loaded PLGA nanoparticles were measured with zeta-sizer by using dynamic light scattering (DLS) technique. The scanning electron microscope (SEM) (Zeiss Supra 50 V) was used for imagining the peptide loaded PLGA nanoparticles. Cell toxicity of nanoparticles was assayed on AGS (gastric adenocarcinoma) cell line. To evaluate mitochondrial activity of cells and toxicity studies, XTT methods were carried out. In this study, we aimed to obtain specific immunological peptide loaded PLGA nanoparticles and characterize the formation with FTIR, zeta sizer and SEM imaging, and evaluate cytotoxicity and carry out molecular docking calculations of peptide-MHC protein in order to enlight in vivo events as vaccine candidate against T. gondii.

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Section: Methodsmentioning

confidence: 99%

Molecular docking of immunogenic peptide ofToxoplasma gondiiand encapsulation with polymer as vaccine candidate

Çakır-Koç

Budama‐Kilinc

Kökcü

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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“…Poly(ethylene oxide)-chitosan. Poly(ethylene oxide) (PEO) is also a biocompatible polymer which has been applied as the cartilage tissue repair [143] and wound dressing [144]. Many nanofibres of the blends of PEO and chitosan have also been produced by electrospinning [145][146][147][148][149][150].…”

Section: Plla-collagenmentioning

confidence: 99%

Recent advances on biomedical applications of scaffolds in wound healing and dermal tissue engineering

Bakhshayesh

Annabi

Khalilov

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

Self Cite

193

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The tissue engineering field has developed in response to the shortcomings related to the replacement of the tissues lost to disease or trauma: donor tissue rejection, chronic inflammation and donor tissue shortages. The driving force behind the tissue engineering is to avoid the mentioned issues by creating the biological substitutes capable of replacing the damaged tissue. This is done by combining the scaffolds, cells and signals in order to create the living, physiological, three-dimensional tissues. A wide variety of skin substitutes are used in the treatment of full-thickness injuries. Substitutes made from skin can harbour the latent viruses, and artificial skin grafts can heal with the extensive scarring, failing to regenerate structures such as glands, nerves and hair follicles. New and practical skin scaffold materials remain to be developed. The current article describes the important information about wound healing scaffolds. The scaffold types which were used in these fields were classified according to the accepted guideline of the biological medicine. Moreover, the present article gave the brief overview on the fundamentals of the tissue engineering, biodegradable polymer properties and their application in skin wound healing. Also, the present review discusses the type of the tissue engineered skin substitutes and modern wound dressings which promote the wound healing.

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“…A novel method which overcomes the above-mentioned shortcomings is the functionalization of polymers with a cavity-containing molecule such as b-cyclodextrin (b-CD) [22,23]. b-CD is a non-toxic cyclic oligosaccharide consisting of seven a-D-glucopyranose units in the form of a ring [24]. The structure of b-CD is intriguing due to its hydrophilic exterior and the hydrophobic inner cavity capable of forming host-guest association with drug molecules of appropriate size and shape [25].…”

Section: Introductionmentioning

confidence: 99%

Folate-molecular encapsulator-tethered biocompatible polymer grafted with magnetic nanoparticles for augmented drug delivery

Ramasamy

David

Enoch

2018

Artificial Cells, Nanomedicine, and Biotechnology

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Magnetic ferrite nanoparticles (MNPs) coated with biocompatible polymers capable of drug loading and release are fascinating nanostructures for delivering anti-cancer drugs. Herein, we report the synthesis and characterization of a novel β-cyclodextrin-folate-tethered dextran polymer. Nickel-zinc ferrite nanoparticles are prepared and coated with the polymer to form a biocompatible hybrid magnetic nanocarrier. To establish the significance of folate unit of the polymer in anticancer activity, a similar derivatized polymer, i.e. β-cyclodextrin-dextran conjugate without folate tether is used for comparison. The size of the hybrid MNPs is ∼20 nm, which is a size suitable for cancer drug targeting. The polymer-coated magnetic nanocarriers are soft ferromagnets as suggested by their narrow magnetic hysteresis loops. The anticancer drug camptothecin (CPT) is loaded on the magnetic nanocarriers. The drug loading efficiency is observed to be above 92%. The nanocarriers show sustained in vitro drug release for above 45 h. The in vitro cytotoxicity studies reveal that the loaded CPT retains its potency in the nanocarrier and the folate-tethered nanocarrier shows better anticancer activity than the one which does not carry a folate unit. The magnetic nanocarrier is suitable for magnetic field-guided drug transport, enhanced drug loading and release and folate receptor-mediated endocytotic uptake of drugs by cancer cells.

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Preparation and characterization of PLGA-β-CD polymeric nanoparticles containing methotrexate and evaluation of their effects on T47D cell line

Cited by 30 publications

References 57 publications

Molecular docking of immunogenic peptide ofToxoplasma gondiiand encapsulation with polymer as vaccine candidate

Molecular docking of immunogenic peptide ofToxoplasma gondiiand encapsulation with polymer as vaccine candidate

Recent advances on biomedical applications of scaffolds in wound healing and dermal tissue engineering

Folate-molecular encapsulator-tethered biocompatible polymer grafted with magnetic nanoparticles for augmented drug delivery

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