Preparation and evaluation of clindamycin phosphate loaded chitosan/alginate polyelectrolyte complex film as mucoadhesive drug delivery system for periodontal therapy

Kiliçarslan, Müge; İlhan, Miray; Inal, Ozge; Orhan, Kaan

doi:10.1016/j.ejps.2018.08.007

Cited by 88 publications

(47 citation statements)

References 56 publications

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“…These systems have some drawbacks including short-term effects and inadequate penetration into the periodontal pockets. Therefore, recent studies have focused on sustained release drug delivery systems [16][17][18][19]. Using a mucoadhesive paste for local release of medications has several important biologic advantages including longer release, intimate contact, increased bioavailability, and specific targeting [20,21].…”

Section: Introductionmentioning

confidence: 99%

Designing a novel chitosan-based periofilm containing metronidazole–ciprofloxacin

et al. 2020

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Periodontitis is the most common periodontal disease. Because of its infectious-inflammatory nature, as well as the difficulty of complete elimination of pathogens from oral cavity, antibiotics have been proposed as adjunctive modalities to control the disease and prevent its recurrence. Systemic antibiotics could lead to a further reduction in probing depth and bleeding on probing. Nowadays, due to their side effects and the emergence of resistant strains of bacteria, there is a tendency to use local antibiotic delivery systems. The aim of this study was to design, develop and test a mucoadhesive film based on chitosan/gelatin containing metronidazole and ciprofloxacin. A mucoadhesive film containing 2% chitosan and gelatin and 0.2% of metronidazole and ciprofloxacin was made by casting method. Laboratory evaluations including drug-releasing pattern through HPTLC, mucoadhesive strength, stability in phosphate buffer, degradation in lysozyme, and water absorption were performed. The results of this study showed that the produced periofilm releases the antibiotics up to 48 h in concentrations higher than MIC. In the presence of lysozyme, a sharp decline in stability observed in the first 4 days, reached to a constant level after seven days. Based on the results of the present study, it could be concluded that applying the proposed system and its drug combination can be a part of the adjunctive periodontal treatment on a daily basis or every 48 h. However, further studies are needed to recommend this system as a routine therapeutic modality.

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Section: Introductionmentioning

confidence: 99%

Designing a novel chitosan-based periofilm containing metronidazole–ciprofloxacin

et al. 2020

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“…This prolonged period and reduced dose at pH 7.4 may be attributed to the electrostatic interaction between functional groups of the chitosan/alginate-STPP complex and the drug, providing good pH sensitivity which improves the controlled release capacity of the Cs-Al NDs [50,51]. On the contrary, the faster release found at pH 1.2 can be related to a higher solubility and swelling capability of Cs-Al NDs, since there was a change in the complex structure due to a possible protonation of the carboxylic and amine groups weakening the bonding with STPP and thus decreasing the π-π interaction between amoxicillin and the Cs-Al NDs structure [51,52]. According to the results, a partial release of amoxicillin was observed which may be attributed by the fact that the nanodisks had a low degradation during the test.…”

Section: Encapsulation Efficacy Andmentioning

confidence: 99%

Ionotropic Gelation Synthesis of Chitosan-Alginate Nanodisks for Delivery System and In Vitro Assessment of Prostate Cancer Cytotoxicity

Patiño-Ruiz

Marrugo

Reyes

et al. 2020

International Journal of Polymer Science

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We report on the synthesis of chitosan-alginate nanodisks (Cs-Al NDs) using a simple approach consisting of the ionotropic gelation method. Sodium tripolyphosphate (STPP) was used as crosslinking agent to promote the electrostatic interaction between amine groups the chitosan and hydroxyl and carboxyl groups of alginate. Scanning electron microscopy (SEM) images provided direct evidence of the morphology of the nanodisks where agglomeration was observed due to the electrostatic interaction between the functional groups. Furthermore, dynamic light scattering (DLS) showed that the hydrodynamic size of the Cs-Al NDs was 227 nm and 152 nm in pH 1.2 and pH 7.4, respectively, which is in agreement with the information observed in the SEM images. The chemical structure is presented mainly the amine and carboxyl groups due to the presence of chitosan and alginate in the nanodisks, respectively, which allow the electrostatic interaction through N-H linkages. According to the X-ray diffraction, we found that the Cs-Al NDs exhibited the typical structure of chitosan and alginate, which lead the formation of polyelectrolyte complexes. We also evaluated the encapsulation of amoxicillin in the nanodisk, obtaining a loading efficiency of 74.98%, as well as a maximum in vitro release amount of 63.2 and 52.3% at pH 1.2 and 7.4, respectively. Finally, the cytotoxicity effect of the Cs-Al nanodisks was performed in human prostatic epithelial PWR-1E and Caucasian prostate adenocarcinoma PC-3 cell lines, in which the cell viability was above 80% indicating low inhibition and determining the Cs-Al NDs as a promising technology for controlled delivery systems.

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“…To observe the release mechanism of α-M from ChAlg HF, an in vitro release study of ChAlg/α-M HF was performed in PBS solution (pH 7.4) at 5,15,30,45,60,90, and 120 min. The concentrations of α-M were measured by UV spectrophotometry at wavelength of 246 nm [24].…”

Section: In Vitro Release Studymentioning

confidence: 99%

“…Patil et al proved that hydrogel based on Ch-Alg formulations had a higher mucoadhesive strength compared to hydrogel based on Alg [14]. In addition, Ch molecular weight in Ch-Alg polyelectrolyte complex film containing clindamycin phosphate affected to adhesiveness of complex films [15]. Mucoadhesive ability of Ch closely related to the structure of Ch, which can interact with mucin predominantly by electrostatic binding [16].…”

Section: Introductionmentioning

confidence: 99%

α-Mangostin Hydrogel Film Based Chitosan–Alginate for Recurrent Aphthous Stomatitis

et al. 2019

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Featured Application: This study highlights a novel application of α-mangostin hydrogel film based chitosan-alginate. α-mangostin has been known as an agent for oral cavity therapy. However, α-mangostin is difficult to be delivered directly to the site. This novel application of hydrogel film-based chitosan-alginate have the potential as carriers of α-mangostin for recurrent aphthous stomatitis therapy.Abstract: Many antiseptic drugs, local anaesthetics, and corticosteroids have been used for effective therapy of recurrent aphthous stomatitis (RAS). However, these drugs have harmful side effects. α-mangostin (α-M), a main compound of mangosteen (Garcinia mangostana L.) peel, has been known as a wound healing agent. In addition, hydrogel film as dressings designed to separate mucosal lesions from the oral environment, and improve the effectiveness of RAS therapy. The purpose of this study was to develop α-M hydrogel film based chitosan-alginate (ChAlg/α-M HF) for RAS. The in silico study by Discovery studio visualizer and AutoDock confirmed that hydrogen bonding between Ch, Alg, and α-M occurred. The results of physicochemical characterizations by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and X-ray diffraction (XRD) indicated that the ChAlg/α-M HF had a lower crystalline form compared to pure α-M. In addition, ChAlg/α-M HF significantly improved the swelling ratio and tensile strength compared to that of ChAlg HF. Moreover, the existence of Alg increased the degradability of Ch, and closely related to the release of α-M from ChAlg HF. The in vitro release study confirmed that the release of α-M from ChAlg/α-M HF was the Fickian diffusion model. Finally, the mucoadhesive study revealed that ChAlg/α-M HF had a good mucoadhesive property. These results suggest that hydrogel film-based chitosan-alginate have the potential as carriers of α-M for RAS therapy.

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Preparation and evaluation of clindamycin phosphate loaded chitosan/alginate polyelectrolyte complex film as mucoadhesive drug delivery system for periodontal therapy

Cited by 88 publications

References 56 publications

Designing a novel chitosan-based periofilm containing metronidazole–ciprofloxacin

Designing a novel chitosan-based periofilm containing metronidazole–ciprofloxacin

Ionotropic Gelation Synthesis of Chitosan-Alginate Nanodisks for Delivery System and In Vitro Assessment of Prostate Cancer Cytotoxicity

α-Mangostin Hydrogel Film Based Chitosan–Alginate for Recurrent Aphthous Stomatitis

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