2005
DOI: 10.21608/jvmr.2005.77994
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Preparation and evaluation of combined inactivated vaccine containing rota, corona viruses, Escherichia coli bacterin and Clostridium perfringens type Ctoxoid (Entero-4)

Abstract: A conclusive study was conducted for preparation and evaluation of combined inactivated entero-4 vaccine containing bovine rotavirus (BRV), bovine coronavirus (BCV), E. coli K 99 and toxoid of C. perfringens type "C".Laboratory and field evaluations were conducted on laboratory animals, calves and late pregnant cows with monitoring the active and passive antibodies in vaccinated cows and their offspring respectively. Laboratory evaluation proved purity, safety and high efficacy of the vaccine without interfere… Show more

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Cited by 2 publications
(4 citation statements)
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“…The MAT results were parallel to the ELISA results (Table 3). These results coincided with that reported by (Collins et al, 1988and Daoud et al, 2003and 2005 who reported that mean anti-E. coli agglutinating antibody titers of vaccinated cows increased by the 4 th week post-vaccination and reached their maximal titers by the 6 th week post-vaccination. Detection of specific antibodies against the prepared BRV, BCV and E. coli K99 lysate antigens using indirect immunoflurescent antibody technique (IFA) was satisfactory (Table 4, Fig.…”
Section: Resultssupporting
confidence: 92%
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“…The MAT results were parallel to the ELISA results (Table 3). These results coincided with that reported by (Collins et al, 1988and Daoud et al, 2003and 2005 who reported that mean anti-E. coli agglutinating antibody titers of vaccinated cows increased by the 4 th week post-vaccination and reached their maximal titers by the 6 th week post-vaccination. Detection of specific antibodies against the prepared BRV, BCV and E. coli K99 lysate antigens using indirect immunoflurescent antibody technique (IFA) was satisfactory (Table 4, Fig.…”
Section: Resultssupporting
confidence: 92%
“…Ten late pregnant Holstein cows, in private dairy farm at El-Nubarria, Alexandria in addition to their offspring calves (n=10) were used in this study. Before vaccination, cows were proved seronegative to BRV and BCV by virus neutralization test (Dauvergene et al, 1983) and to E. coli by microagglutination test (Collins et al, 1988). They were vaccinated twice with the local inactivated entero-3 vaccine at 8 and 5 weeks before parturition.…”
Section: Methodsmentioning
confidence: 99%
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“…El-Sayed et al (2011) prepared a combined inactivated bovine Rotavirus (BRV), Coronavirus, and E. coli strain K99 and cited that the antibody titers against E. coli revealed that the antibodies increased at the 4 th -week post-vaccination and reached the highest titer at the 4 th month, then began to decrease. Daoud et al (2005), who prepared and evaluated a combined inactivated vaccine containing E. coli (K99), C. perfringens, Corona, and Rotaviruses, concluded that the maximal antibody titer obtained for E. coli was at the 8 th -week post-vaccination. There was no immunological interference between E. coli bacterin and the other antigens in the inactivated vaccine.…”
Section: Discussionmentioning
confidence: 99%