Preparation of diagnostic lysate antigens for rapid evaluation of local entero-3 vaccine

Abstract

Studies were conducted to determine the utility of lysate antigens for rapid evaluation of the local entero-3 vaccine, antigens were prepared from cell cultures infected with bovine rota virus (BRV) and bovine corona virus (BCV) as well as from Enterotoxigenic E. coli strain K99. Prepared antigens were tested with field serum samples collected from both late pregnant entero-3 vaccinated cows and their offsprings using different serological assays including: microagglutination test, indirect ELISA and immunofluorescent antibody technique. Results of this endeavor were correlated to that of the standard virus neutralization test. The locally prepared antigens were proved useful for vaccine evaluation. Moreover, these antigens are recommended for both detection and assessment post vaccination or post infection of sero-conversion against BRV, BCV and E. coli.

Efficacy of the E. Coli whole cell lysates for improving immunogenic quality of the local entero-3 vaccine

“…Growth continued for 24 h at 37°c and the bacterial cells were collected by centrifugation at 6000 rpm, 4°c for 30 min. The bacterial cell pellets were washed twice in sterile normal saline then solubilized with lysis buffer thus producing translucent lysates (El-kholy et al, 2005) The whole bacterial cell lysate (WBCL) were kept at -20°c until used.…”