Preparation and Evaluation of Iron Oxide Nanoparticles for Treatment of Iron Deficiency Anemia

Abstract

Objective: The objective of this research was to formulate and evaluate iron oxide nanoparticles for treatment of iron deficiency anemia (IDA). Methods:Iron oxide nanoparticles were prepared by co-precipitation method and stabilized by coating with folic acid or chitosan. The prepared nanoparticles were characterized in vitro for morphology, particle size, zeta potential, crystallinity and ultraviolet-visible (UV-Vis) absorption. In vivo studies were performed to evaluate the efficacy of the prepared nanoparticles in treating iron-deficient anemic rats compared to the commercial iron product. Results:In vitro results revealed that particle sizes were 65.95±5 nm, 220.2±12 nm and 295.3±19 nm for uncoated iron oxide nanoparticles, folic acid-coated iron oxide nanoparticles and chitosan coated iron oxide nanoparticles, respectively. UV-Vis absorption spectrum and x-ray diffraction (XRD) patterns confirmed that the prepared nanoparticles were iron oxide nanoparticles. In vivo results indicated that folic acid-coated iron oxide nanoparticles showed effective restorative action, returning haemoglobin (Hb) concentration to normal levels, where not only complete recovery of Hb within short time from the anemic state to the high normal level, but also improved Hb concentrations compared to the commercial iron product. Conclusion:The results obtained in this research work clearly indicated a promising potential of folic acid-coated iron oxide nanoparticles for the effective treatment of IDA.

Iron Oxide Nanoparticles Versus Ferrous Sulfate In Treatment of Iron Deficiency Anemia In Rats

“…By treatment with iron oxide nanoparticles or ferrous sulfate, there was no significant change in the blood indices than control. Our result matched with Hashem et al [7] who reported a significant increase in red blood cell indices in iron oxide nanoparticles group when compared to anemic group which may be due to the effectiveness of iron oxide nanoparticles in treatment of anemia.…”

“…It is composed of 10 % sunflower oil, 4% salt mixture, 1% vitamin mixture, 2 % choline chloride, 12 % DL-methionine and cornstarch up to 100 g [7].…”

Umbilical Cord Blood Hematology in Relation With Maternal Anemia: A Preliminary Study

“…Iron deficiency anemia is a most common nutritional disorder and serious public health concern in developing countries, and maternal iron deficiency anemia increases the incidence of maternal mortality and morbidity as well it also increases the incidence of fetal growth retardation, premature delivery, and neurodevelopment of the fetus [6,7].…”

The effect of PEGylated iron oxide nanoparticles on sheep ovarian tissue: An ex-vivo nanosafety study

“…As we know, IONP have many applications in medicine including: enhance targeting for drug delivery [32], hyperthermia to complement treatment of various tumor types [33], promote transient opening of the blood-brain barrier [34], enhance MRI contrast for medical imaging [35]. In addition, IONP-based drugs are FDA-approved to treat iron deficiency [36,37]. The ovary is the most important and arguably the most delicate female reproductive organ that feeds from the main artery which in turn stems from ovarian branches of the aorta [13].…”