Preparation and physicochemical properties of various soybean lecithin liposomes using supercritical reverse phase evaporation method

Imura, Tomohiro; Otake, Katsuto; Hashimoto, Satoru; Gotoh, Toshihiro; Yuasa, Makoto; Yokoyama, Shoko; Sakai, Hideki; Rathman, James F.; Abe, Masahiko

doi:10.1016/s0927-7765(02)00048-6

Cited by 80 publications

(39 citation statements)

References 22 publications

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“…The lipids used have very low solubility in carbon dioxide; therefore the organic co-solvent is incorporated to improve the solubility of the lipid in the dense gas phase. Although organic co-solvent is used in the scRPE method, non-toxic solvents can be Otake et al formed ellipsoidal LUVs that ranged in size from 100 nm-1.2 µm and the vesicles became spherical as the size was decreased (29,38). It was suggested that the ellipsoidal shape is caused by the lower stability of the LUVs in comparison to spherical MLVs.…”

Section: Supercritical Reverse Phase Evaporation Processmentioning

confidence: 99%

Conventional and Dense Gas Techniques for the Production of Liposomes: A Review

2008

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Abstract. The aim of this review paper is to compare the potential of various techniques developed for production of homogenous, stable liposomes. Traditional techniques, such as Bangham, detergent depletion, ether/ethanol injection, reverse-phase evaporation and emulsion methods, were compared with the recent advanced techniques developed for liposome formation. The major hurdles for scaling up the traditional methods are the consumption of large quantities of volatile organic solvent, the stability and homogeneity of the liposomal product, as well as the lengthy multiple steps involved. The new methods have been designed to alleviate the current issues for liposome formulation. Dense gas liposome techniques are still in their infancy, however they have remarkable advantages in reducing the use of organic solvents, providing fast, single-stage production and producing stable, uniform liposomes. Techniques such as the membrane contactor and heating methods are also promising as they eliminate the use of organic solvent, however high temperature is still required for processing.

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Section: Supercritical Reverse Phase Evaporation Processmentioning

confidence: 99%

Conventional and Dense Gas Techniques for the Production of Liposomes: A Review

2008

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“…As the material of the carrier, it is also required to be non-toxic, biocompatible and biodegradable 4 . Liposomal delivery systems are effective vehicles which have been paid considerable attention in pharmaceutical, cosmetic, food and nutritional industries [5][6][7][8][9] . Liposomes are colloidal structures formed by the self-assembly of Amphiphilic lipid molecules in solution which can encapsulate lipid-soluble substances 5,8 .…”

Section: Introductionmentioning

confidence: 99%

Chitosan-coated nanoliposome as vitamin E carrier

Liu

Park

2009

Journal of Microencapsulation

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A new nutraceutical carrier, chitosan-coated nano-size liposome, which is itself bioactive, is made from phosphatidylcholine (pc) and cholesterol by the sonication method. Different factors related to the size of the liposomes such as the ratio of the pc and cholesterol and the time of sonication are investigated. At the 40 : 60 ratios of pc and cholesterol with 12 min sonication, the smallest liposomes, with mean diameter ∼82 nm, were obtained. Incorporation of chemically labile active ingredients into the chitosan-coated liposomes prevented chemical degradation, which is shown for vitamin E (VE). After VE was loaded to the liposomes, the size of chitosan-coated liposomes increased to 144 nm. The loading efficiency and payload of VE were investigated by loading different amounts of VE to the liposomes which were prepared with different ratios of pc and cholesterol. Chitosan-coated nano-coated nano-size liposomes appeared to be promising VE carriers, with highest loading efficiency over 99% and payload over 27%. The stability of VE-loaded liposomes suspension during the 8 weeks storage is over 90% under 4°C.

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“…It is also because of their ability to bring active material directly to the active site, known as targeted delivery 6 . Research on liposomes from natural phospholipid that were derived from soybeans has been widely reported [7][8][9][10] but research on liposomes of coconut and sesame phospholipids so far only a few 11 .…”

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confidence: 99%