Preparation and study of 3D printed dipyridamole/β-tricalcium phosphate/ polyvinyl alcohol composite scaffolds in bone tissue engineering

Xu, Zhimin; Wang, Ningning; Ma, Yuantai; Dai, Huanyan; Han, Bing

doi:10.1016/j.jddst.2021.103053

Cited by 9 publications

(4 citation statements)

References 56 publications

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“…Xu and team incorporated the adenosine receptor indirect agonist dipyridamole into a poly(vinyl alcohol)/β-TCP composite biomaterial using 3D-printing technology. The findings revealed that the inclusion of the drug enhanced the hydrophilicity of the scaffold, promoted cell proliferation and adhesion and notably stimulated the osteogenic differentiation of stem cells [68]. LG-containing scaffolds show a 15% increase in mechanical strength.…”

Section: Beta-tricalcium Phosphate (β-Tcp)-based 3d-printed Scaffoldsmentioning

confidence: 99%

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Calcium Phosphate Biomaterials for 3D Bioprinting in Bone Tissue Engineering

Tolmacheva,

Bhattacharyya,

Noh

2024

Biomimetics

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Three-dimensional bioprinting is a promising technology for bone tissue engineering. However, most hydrogel bioinks lack the mechanical and post-printing fidelity properties suitable for such hard tissue regeneration. To overcome these weak properties, calcium phosphates can be employed in a bioink to compensate for the lack of certain characteristics. Further, the extracellular matrix of natural bone contains this mineral, resulting in its structural robustness. Thus, calcium phosphates are necessary components of bioink for bone tissue engineering. This review paper examines different recently explored calcium phosphates, as a component of potential bioinks, for the biological, mechanical and structural properties required of 3D bioprinted scaffolds, exploring their distinctive properties that render them favorable biomaterials for bone tissue engineering. The discussion encompasses recent applications and adaptations of 3D-printed scaffolds built with calcium phosphates, delving into the scientific reasons behind the prevalence of certain types of calcium phosphates over others. Additionally, this paper elucidates their interactions with polymer hydrogels for 3D bioprinting applications. Overall, the current status of calcium phosphate/hydrogel bioinks for 3D bioprinting in bone tissue engineering has been investigated.

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Section: Beta-tricalcium Phosphate (β-Tcp)-based 3d-printed Scaffoldsmentioning

confidence: 99%

“…~500 µm [68] 4.3. Biphasic Calcium Phosphate (BCP)-Based 3D-Printed Scaffolds BCP, as well as HAp and β-TCP, are widely used biomaterials for bone repair in clinical applications.…”

Section: Beta-tricalcium Phosphate (β-Tcp)-based 3d-printed Scaffoldsmentioning

confidence: 99%

Calcium Phosphate Biomaterials for 3D Bioprinting in Bone Tissue Engineering

Tolmacheva,

Bhattacharyya,

Noh

2024

Biomimetics

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“…Modern approaches include the development of biocompatible scaffolds with mechanical characteristics close to bone and capable of supporting the biomineralization and growth of the new vessels. To date, a wide range of scaffolds based on bioceramics [ 27 , 28 ], porous polymeric [ 23 , 26 ] and composite materials [ 29 , 30 , 31 , 32 ], have been developed. Among the reported polymer scaffolds, 3D-printed materials [ 33 ] based on biocompatible and biodegradable thermoplastic aliphatic polyesters, such as poly(lactic acid) (PLA), poly(lactide-co-glycolide) (PLGA), poly(ε-caprolactone) (PCL), poly(3-hydoxybutyric acid) (PHB), etc., have demonstrated the appropriate mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Autografts and Biodegradable 3D-Printed Composite Scaffolds with Osteoconductive Properties for Tissue Regeneration in Bone Tuberculosis

et al. 2023

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Tuberculosis remains one of the major health problems worldwide. Besides the lungs, tuberculosis affects other organs, including bones and joints. In the case of bone tuberculosis, current treatment protocols include necrectomy in combination with conventional anti-tuberculosis therapy, followed by reconstruction of the resulting bone defects. In this study, we compared autografting and implantation with a biodegradable composite scaffold for bone-defect regeneration in a tuberculosis rabbit model. Porous three-dimensional composite materials were prepared by 3D printing and consisted of poly(ε-caprolactone) filled with nanocrystalline cellulose modified with poly(glutamic acid). In addition, rabbit mesenchymal stem cells were adhered to the surface of the composite scaffolds. The developed tuberculosis model was verified by immunological subcutaneous test, real-time polymerase chain reaction, biochemical markers and histomorphological study. Infected animals were randomly divided into three groups, representing the infection control and two experimental groups subjected to necrectomy, anti-tuberculosis treatment, and plastic surgery using autografts or 3D-composite scaffolds. The lifetime observation of the experimental animals and analysis of various biochemical markers at different time periods allowed the comparison of the state of the animals between the groups. Micro-computed tomography and histomorphological analysis enabled the evaluation of osteogenesis, inflammation and cellular changes between the groups, respectively.

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“…There was no change in the strength of the scaffold after new cells were grown into the structure. Therefore, the scaffolds developed were concluded to be more bioactive, with cell proliferation abilities [ 28 ]. Though previous studies have shown TCP as a material for scaffolds with active bone regeneration, the influence of the TCP ink’s rheological characteristics on extrudability, dimensional stability, and drying behavior through the DIW process has not been studied in any systematic way.…”

Section: Introductionmentioning

confidence: 99%

Rheological Behavior and Printability Study of Tri-Calcium Phosphate Ceramic Inks for Direct Ink Writing Method

et al. 2023

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In the biomedical industry, tricalcium phosphate is a bioceramic substance that is frequently employed in the fabrication of scaffolds and bone structures. Fabrication of porous ceramic structures using conventional manufacturing techniques is very challenging because of the brittle nature of the ceramics, which has led to a newly adapted direct ink writing additive manufacturing method. This work investigates the rheology and extrudability of TCP inks to produce near-net-shape structures. Viscosity and extrudability tests found that stable TCP: Pluronic ink of 50 vol.% was more reliable compared to other tested inks prepared from a functional polymer group polyvinyl alcohol. A line study was carried out to identify the printing parameters suitable for printing structures from the selected ink with lesser dimensional error. Printing speed 5 mm/s and extrusion pressure 3 bar was found suitable to print a scaffold through a nozzle of 0.6 mm, keeping the stand-off distance equal to the nozzle diameter. The printed scaffold was further investigated for its physical and morphological structure of the green body. A suitable drying behavior was studied to remove the green body without cracking and wrapping before the sintering of the scaffold.

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Preparation and study of 3D printed dipyridamole/β-tricalcium phosphate/ polyvinyl alcohol composite scaffolds in bone tissue engineering

Cited by 9 publications

References 56 publications

Calcium Phosphate Biomaterials for 3D Bioprinting in Bone Tissue Engineering

Calcium Phosphate Biomaterials for 3D Bioprinting in Bone Tissue Engineering

Comparison of Autografts and Biodegradable 3D-Printed Composite Scaffolds with Osteoconductive Properties for Tissue Regeneration in Bone Tuberculosis

Rheological Behavior and Printability Study of Tri-Calcium Phosphate Ceramic Inks for Direct Ink Writing Method

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