Preparation, chain conformation and anti-tumor activities of water-soluble phosphated (1→3)-α-d-glucan from Poria cocos mycelia

Huang, Qilin; Zhang, Lina

doi:10.1016/j.carbpol.2010.09.057

Cited by 70 publications

(23 citation statements)

References 37 publications

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“…The anti-tumor and immunomodulation activity could be enhanced by chemical modification (i.e. sulfation, phosphorylation and selenylation) of polysaccharides (Huang & Zhang, 2011;Gao et al, 2016;Chen et al, 2014b;Jiang et al, 2015). However, the correlation between structure aspects and anti-tumor activity was unclear.…”

Section: Anti-tumor Activity In Vitromentioning

confidence: 99%

“…Some low MW polysaccharides (such as schizophyllan) exhibited similar anti-tumor activities compared to polysaccharides with higher MW (Meng, Liang & Luo, 2016). Moreover, phosphated glucan from Poria cocos mycelia presented stronger anti-tumor activities due to its expanded chain conformation after modification (Huang & Zhang, 2011). In order to study the effect of selenylation on the bioactivity of modified polysaccharides, ASP and SeASP2 were tested in the CCK-8 assay to assess its anti-tumor potential in human hepatocellular carcinoma HepG-2 cells, lung adenocarcinom A549 cells and cervical squamous carcinoma Hela cells in vitro.…”

Section: Anti-tumor Activity In Vitromentioning

confidence: 99%

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Synthesis of selenium-containing Artemisia sphaerocephala polysaccharides: Solution conformation and anti-tumor activities in vitro

Wang

Bao

et al. 2016

Carbohydrate Polymers

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It has been reported in our previous work that selenized Artemisia sphaerocephala polysaccharides (SeASPs) with the Se content range of 168-1703μg/g were synthesized by using Na2SeO3/HNO3/BaCl2 system. In the present work, the solution property of SeASP was studied by using size exclusion chromatography combined with multi angle laser light scattering (SEC-MALLS). A decrease in df values indicated that SeASPs with different conformational features that were highly dependent on MW. SeASPs exhibited a more rigid conformation (df value of 1.29-1.52) in low molecular weight range (MW of 1.026-1.426×10(4)g/mol) and compact spherical conformation in high molecular weight range (MW of 2.268-4.363×10(4)g/mol). It could be due to the degradation of polysaccharide chains in HNO3, which was supported in monosaccharide composition analysis. Congo red (CR) spectrophotometric method and atomic force microscopy (AFM) results also confirmed the conformational transition and the evidence on the shape of the rigid chains. In vitro anti-tumor assays, SeASP2 displayed greater anti-proliferative effects against three tumor cell lines (hepatocellular carcinoma HepG-2 cells, lung adenocarcinom A549 cells and cervical squamous carcinoma Hela cells) in a dose-dependent manner. This suggested that selenylation could significantly enhance the anti-tumor activities of polysaccharide derivatives in vitro.

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Section: Anti-tumor Activity In Vitromentioning

confidence: 99%

Section: Anti-tumor Activity In Vitromentioning

confidence: 99%

Synthesis of selenium-containing Artemisia sphaerocephala polysaccharides: Solution conformation and anti-tumor activities in vitro

Wang

Bao

et al. 2016

Carbohydrate Polymers

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“…The mice were killed after the tumors were allowed to grow for another 7 days, and the tumors were excised and weighed. The inhibition ratio (ξ) was calculated as follows (Huang & Zhang, 2011):…”

Section: In Vivo Antitumor Activity Assaymentioning

confidence: 99%

Structure characterization and antitumor activity of an α β-glucan polysaccharide from Auricularia polytricha

Song

2012

Food Research International

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“…Several studies have shown that both natural and artificially modified phosphorylated polysaccharides have specified medicinal values, due to the fact that the existence of charged phosphate groups can improve water solubility, change molecular weight, and modify chain conformation of polysaccharides. Chen et al [72] and Huang et al [73] studied the chain conformation and antitumor activity of phosphate modified Tuckahoe β-glucan and Tuckahoe hyphae α-glucan, respectively. In 0.15 mol/L NaCl solution, β-glucan phosphate showed a relatively extended compliant chain conformation, and Tuckahoe hyphae α-glucan phosphate showed a more extended semi-rigid chain conformation.…”

Section: Phosphorylation Modificationmentioning

confidence: 99%

Effect of the Modifications on the Physicochemical and Biological Properties of β-Glucan—A Critical Review

Yuan

Lan

et al. 2019

Molecules

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β-Glucan exhibits many biological activities and functions such as stimulation of the immune system and anti-inflammatory, anti-microbial, anti-infective, anti-viral, anti-tumor, anti-oxidant, anti-coagulant, cholesterol-lowering, radio protective, and wound healing effects. It has a wide variety of uses in pharmaceutical, cosmetic, and chemical industries as well as in food processing units. However, due to its dense triple helix structure, formed by the interaction of polyhydroxy groups in the β-d-glucan molecule, it features poor solubility, which not only constrains its applications, but also inhibits its physiological function in vivo. One aim is to expand the applications for modified β-glucan with potential to prevent disease, various therapeutic purposes and as health-improving ingredients in functional foods and cosmetics. This review introduces the major modification methods required to understand the bioactivity of β-glucan and critically provides a literature survey on the structural features of this molecule and reported biological activity. We also discuss a new method to create novel opportunities to exploit maximally various properties of β-glucan, namely ultrasound-assisted enzymatic modification.

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Preparation, chain conformation and anti-tumor activities of water-soluble phosphated (1→3)-α-d-glucan from Poria cocos mycelia

Cited by 70 publications

References 37 publications

Synthesis of selenium-containing Artemisia sphaerocephala polysaccharides: Solution conformation and anti-tumor activities in vitro

Synthesis of selenium-containing Artemisia sphaerocephala polysaccharides: Solution conformation and anti-tumor activities in vitro

Structure characterization and antitumor activity of an α β-glucan polysaccharide from Auricularia polytricha

Effect of the Modifications on the Physicochemical and Biological Properties of β-Glucan—A Critical Review

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