Preparation, characterization and biological test of 3D-scaffolds based on chitosan, fibroin and hydroxyapatite for bone tissue engineering

Lima, Paulo Autran Leite; Resende, Cristiane Xavier; Soares, Glória Dulce de Almeida; Anselme, Karine; Almeida, Luís Eduardo

doi:10.1016/j.msec.2013.04.026

Cited by 71 publications

(38 citation statements)

References 45 publications

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“…SF and CS have been applied to tissue engineering widely, e.g. bone reconstruction and skin regeneration [Deng et al, 2013;Gu et al, 2013;Lima et al, 2013]. In this work, we successfully used a 75: 25 SFCS blend scaffold as a transparent artificial corneal stroma for cellular seeding and in vivo implantation in an animal model for the first time.…”

Section: Discussionmentioning

confidence: 99%

Use of a Silk Fibroin-Chitosan Scaffold to Construct a Tissue-Engineered Corneal Stroma

Guan

Tang

et al. 2013

Cells Tissues Organs

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Purpose: To construct a scaffold using silk fibroin (SF) and chitosan (CS) that could replace the corneal stroma with the biological characteristics of the scaffold materials still intact. Methods: To develop an organotypic corneal stroma, SF and CS were chosen to synthesise the tissue-engineered bioscaffold. We cultured primary rabbit corneal epithelial cells and corneal stromal cells in vitro. Keratocytes were used to assess cytotoxicity on SF-CS (SFCS) blends, which was determined by a Cell Counting Kit-8 assay. The corneal lamellar scaffolds were developed with sequential culture techniques to form cell-scaffold constructs. Implantation was tested in 15 New Zealand White rabbits. The corneal substitutes were analysed by light and electron microscopy. Results: The reconstructed lamellar cornea was comparable to native tissue, with high levels of K3/12 expression in the corneal epithelial cells and vimentin in the stromal cells; moreover, the morphology and the position of the cells could be distinguished by histological methods. There was no sign of any immune reaction in or around the transplanted discs 12 weeks after implantation. Conclusion: A SFCS scaffold might be a suitable blend for corneal tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Use of a Silk Fibroin-Chitosan Scaffold to Construct a Tissue-Engineered Corneal Stroma

Guan

Tang

et al. 2013

Cells Tissues Organs

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“…Another group prepared porous 3D-scaffolds based on chitosan (CHI), chitosan/silk fibroin (CHI/SF) and chitosan/silk fibroin/hydroxyapatite (CHI/SF/HA). 66 SF/HA scaffolds has been previously reported to be unsuitable for bone tissue engineering, due to insufficient formability and inflexibility. 67 The characteristics imparted by the nature of the chitosan polymer could improve these characteristics.…”

Section: Methodsmentioning

confidence: 99%

Scaffold Design for Bone Regeneration

Polo-Corrales¹,

Latorre-Esteves²,

Ramı́rez-Vick³

2014

j. nanosci. nanotech.

788

502

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The use of bone grafts is the standard to treat skeletal fractures, or to replace and regenerate lost bone, as demonstrated by the large number of bone graft procedures performed worldwide. The most common of these is the autograft, however, its use can lead to complications such as pain, infection, scarring, blood loss, and donor-site morbidity. The alternative is allografts, but they lack the osteoactive capacity of autografts and carry the risk of carrying infectious agents or immune rejection. Other approaches, such as the bone graft substitutes, have focused on improving the efficacy of bone grafts or other scaffolds by incorporating bone progenitor cells and growth factors to stimulate cells. An ideal bone graft or scaffold should be made of biomaterials that imitate the structure and properties of natural bone ECM, include osteoprogenitor cells and provide all the necessary environmental cues found in natural bone. However, creating living tissue constructs that are structurally, functionally and mechanically comparable to the natural bone has been a challenge so far. This focus of this review is on the evolution of these scaffolds as bone graft substitutes in the process of recreating the bone tissue microenvironment, including biochemical and biophysical cues.

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“…Most of the numerous studies of direct applications of chitosan in 3D scaffolds have focused on blending 3D composite scaffolds with other materials for permanent implantation in the human body [2,3]. Chitosan has been chemically modified for use in 3D scaffolds such as chitosan hydrogels, chitosan sponges, and 2D scaffolds such as chitosan films, nanofiber membranes, by way of sulfonation, phosphorylation, quaternization, and cross-linking methods [4][5][6][7][8][9][10][11]. However, this study is the first to apply a grafting method in a 3D scaffold.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Cell Culture of a Grafted Chitosan Scaffold for Tissue Engineering

Hsieh¹,

Liau²,

Li³

2015

International Journal of Polymer Science

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Poly(vinyl alcohol) (PVA) was grafted to chitosan to form a porous scaffold. The PVA-g-chitosan 3D scaffold was then observed by Fourier transform infrared spectroscopy (FT-IR). The water absorbency of PVA-g-chitosan was increased 370% by grafting. Scanning electron microscope (SEM) observations of the material revealed that the 3D scaffold is highly porous when formed using a homogenizer at 300 rpm. Compression testing demonstrated that as the amount of chitosan increases, the strength of the 3D scaffold strength reached showed that, by increasing the amount of chitosan, the strength of the 3D scaffold could be increased to 16 × 10 −1 MPa. Over 35 days of enzymatic degradation, the 3D scaffold was degraded by various enzymes at rates of up to 10%. In vitro tests showed good cell proliferation and growth in the 3D scaffold.

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Preparation, characterization and biological test of 3D-scaffolds based on chitosan, fibroin and hydroxyapatite for bone tissue engineering

Cited by 71 publications

References 45 publications

Use of a Silk Fibroin-Chitosan Scaffold to Construct a Tissue-Engineered Corneal Stroma

Use of a Silk Fibroin-Chitosan Scaffold to Construct a Tissue-Engineered Corneal Stroma

Scaffold Design for Bone Regeneration

Characterization and Cell Culture of a Grafted Chitosan Scaffold for Tissue Engineering

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