Preparation, characterization and pharmacokinetic studies of tacrolimus-dimethyl-β-cyclodextrin inclusion complex-loaded albumin nanoparticles

Liposomes containing bile salts as novel ocular delivery systems for tacrolimus (FK506): in vitro characterization and improved corneal permeation

“…4 Clinical studies have shown that FK506 delivered topically is able to inhibit immunological rejection after high-risk corneal and limbal grafts. 5 However, FK506 has difficulty penetrating the corneal epithelium and accumulates in the corneal stroma due to its poor water solubility (1.3 µg/mL) 6 and relatively high molecular weight (804.02 g/mol). For inhibition of immunological rejection after corneal grafts, eye drops are a better choice over intraocular dosage formulations.…”

Bovine serum albumin nanoparticles for delivery of tacrolimus to reduce its kidney uptake and functional nephrotoxicity

“…An HPLC system (HP1100, Agilent, USA) with a UV detector set at 220 nm was used to determine the concentration of TAC in the supernatant (Gao et al, 2012). The separation of TAC in 20 mL of samples was performed on a reversed phase column (ODS C18, 5 mm, 4.6 mm  250 mm; Dikma, Beijing, China) at 50 C, eluting with a mixture of acetonitrile and water at a ratio of 3:1 (v/v) at a flow rate of 1.0 mL/min.…”

Preparation, characterization, molecular docking and in vitro evaluation of two novel morin hydrate/CD inclusion complexes

“…Characterization of these complexation demonstrated that the complexes enable an increase in the solubility and bioavailability of MH. Through UV-Vis spectroscopy, 1 H NMR, 2D NMR (ROESY), SEM, FT-IR, DSC, TGA, as well as XRD analysis, their behavior, characterization and binding ability were researched in both aqueous solution and in the solid state [15][16][17]. Meanwhile, we obtained the 1:1 binding model of CDs and MH by means of phase solubility studies, and the inclusion geometry was calculated through molecular docking.…”