Preparation of Conjugated Linoleic Acid Microemulsions and their Biodistribution

Kishita, Kazuaki; Ibaraki, Kayo; Itakura, Shoko; Yamasaki, Yumi; Nishikata, Naoko; Yamamoto, Kiyomi; Shimizu, Masataka; Nishiyama, Kazuo; Yamasaki, Masao

doi:10.5650/jos.ess16099

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…This information implies that an edible plant derived might be vectors of small RNA to predator cells result in the regulation of the gene expression. Artificial nanoparticles and animal-derived exosomes are being attracted as putative materials for drug delivery [ 23 ] and edible plant-derived nanoparticles containing plant-derived miRNAs are also emerging candidates for the therapeutics of various diseases [ 24 , 25 ]. Of note, plant RNAs are capable of modulating immune function such as the regulation of response to TLR-4 stimulation in dendritic cells [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, However, Their Intracellular Incorporation by Endocytosis Was Not Involved in This Effect on RAW264 Cells

Yamasaki

Furusho

et al. 2021

Molecules

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The aim of this study was to evaluate the involvement of nanoparticles prepared from Allium cepa L. as anti-inflammatory agents. In the present study, we identified nanoparticles from Allium cepa L. using the ultracentrifugation exosome purification method. The nanoparticles were referred to as 17,000× g and 200,000× g precipitates, and they contained quercetins, proteins, lipids, and small-sized RNA. The nanoparticles inhibited nitric oxide production from lipopolysaccharide (LPS)-stimulated RAW264 cells without cytotoxic properties. Cellular incorporation was confirmed by laser microscopic observation after PKH26 staining. The inhibition of caveolae-dependent endocytosis and macropinocytosis significantly prevented the incorporation of the nanoparticles but had no effect on the inhibition of nitric oxide in RAW264 cells. Collectively, the identified nanoparticles were capable of inhibiting the LPS response via extracellular mechanisms. Taken together, the way of consuming Allium cepa L. without collapsing the nanoparticles is expected to provide an efficient anti-inflammatory effect.

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Section: Discussionmentioning

confidence: 99%

Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, However, Their Intracellular Incorporation by Endocytosis Was Not Involved in This Effect on RAW264 Cells

Yamasaki

Furusho

et al. 2021

Molecules

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“…10 To overcome these problems, it is necessary to protect/ encapsulate it by using efficient delivery systems. In order to protect PUFAs against oxidation, different encapsulation systems have been developed including protein particles, 9 micro emulsions, 11 liposomes, 12 hydrogels 13 and systems based in polymers. 14 Food proteins are biopolymers with excellent properties for encapsulation of bioactive compounds.…”

Section: Introductionmentioning

confidence: 99%

Simulated gastrointestinal digestion of inclusion complexes based on ovalbumin nanoparticles and conjugated linoleic acid

et al. 2019

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“…An extent of the biodistributed drug is affected by the macrophages of liver, spleen, and lungs with the result of RES uptake [ 71 ]. HCO-60, a pharmaceutically modified castor oil in conjugated linoleic acid microemulsion was effective as co-surfactant against RES uptake [ 72 ]. Combination of carriers such as capmul and myvacet oil used thereof in particulate systems accounts largely for biodistribution of emulsified systems [ 73 , 74 ].…”

Section: Introductionmentioning

confidence: 99%

Biopharmaceutical insights of particulate emulsified systems - a prospective overview

Katamreddy

Yalavarthi

et al. 2018

Lipids Health Dis

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During the twenty-first century, drug discovery is expanding rapidly and a large number of chemical moieties are recognized. Many of them are poorly soluble and hence related biopharmaceutical constraints are to be addressed systematically. Among novel approaches to resolving biopharmaceutical issues, micro- and nano-emulsified systems serve as the best strategy for delivering both hydrophobic and hydrophilic drugs owing to their greater solubilization and transportation capabilities. Of late, the unique physical and biopharmaceutical properties of these liquid isotropic homogenous systems have gained substantive research importance. In addition nano/micro lipid systems share structural and functional similarity with that of the physiological lipids which offer better tolerance ability in the body. In this context, this article provides information on the historical emergence of particulate emulsified systems, importance and rationale of selection of carriers. It also encompasses the physicochemical principles that are responsible for the elevation of therapeutic outcomes of delivery systems. Detailed and schematic absorption of these drug delivery systems is explained here. Gastro-intestinal biochemistry necessary in the understanding of digestion process, lipolytic products formed, micellar structures, enzymes, transporters, mechanism of cell uptake involved after subsequent oral absorption are also emphasized. In addition, this article also explains disposition and pharmacokinetic properties of emulsified systems with real-time therapeutic research outcomes. The influence of biochemical compositions and biopharmaceutical principles on absorption and disposition patterns of ME/NEs was described in the article for the interest of readers and young researchers.

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Preparation of Conjugated Linoleic Acid Microemulsions and their Biodistribution

Cited by 7 publications

References 21 publications

Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, However, Their Intracellular Incorporation by Endocytosis Was Not Involved in This Effect on RAW264 Cells

Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, However, Their Intracellular Incorporation by Endocytosis Was Not Involved in This Effect on RAW264 Cells

Simulated gastrointestinal digestion of inclusion complexes based on ovalbumin nanoparticles and conjugated linoleic acid

Biopharmaceutical insights of particulate emulsified systems - a prospective overview

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