Preparation of cucumber seed peptide-calcium chelate by liquid state fermentation and its characterization

Wang, Xu; Gao, Ang; Chen, Yue; Zhang, Xiaoyuan; Li, Shuhong; Chen, Ye

doi:10.1016/j.foodchem.2017.02.121

Cited by 119 publications

(88 citation statements)

References 39 publications

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“…After chelating with Fe 2+ , the surface of DPs‐Fe was coarse, which was a compact particle aggregate. It might be that the ‘bridging role’ was formed in the process of the chelating reaction between Fe 2+ and the carboxyl group of DPs …”

Section: Resultsmentioning

confidence: 99%

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Effect of duck egg white peptide‐ferrous chelate on iron bioavailability in vivo and structure characterization

Shi

et al. 2018

J Sci Food Agric

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BACKGROUND In order to utilize the industrial by‐product ‘salted duck egg white’ as novel iron additives, the effects of desalted duck egg white peptides‐ferrous chelate (DPs‐Fe) on the promotion of iron uptake and the structure were investigated. RESULTS Different doses of DPs‐Fe were given and iron sulfate (FeSO4) was used as a positive control. After three weeks, hemoglobin (Hb), hematocrit (HCT), red blood cells (RBCs), mean corpuscular volume (MCV), serum iron (SI) and serum ferritin (SF) in iron‐deficiency anemia (IDA) rats could be significantly (P < 0.05) increased to the normal levels by DPs‐Fe. The gene expressions of divalent metal transporter 1 (DMT1), ferroportin 1 (FPN1) and Hepcidin could be regulated by DPs‐Fe. Additionally, DPs‐Fe was formed during the chelation process and the structure was characterized. Eight crucial iron‐chelating peptides of duck egg white peptides (DPs) were identified by HPLC‐ESI‐MS/MS, such as Pro‐Val‐Glu‐Glu and Arg‐Ser‐Ser. It indicated that Glu, Asp, Lys, His, Ser, Cys residues might play crucial roles in the chelating of DPs with iron. CONCLUSION DPs‐Fe could be a potential iron supplement, and the Glu, Asp, Lys, His played important roles in binding iron and promoting iron uptake. This research expands the understanding of iron uptake by DPs and provides an opportunity for recycling a discarded processing byproduct. © 2018 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

“…It might be that the 'bridging role' was formed in the process of the chelating reaction between Fe 2+ and the carboxyl group of DPs. 17 Energy dispersive spectroscopy is an effective method to analyse the surface elemental composition. 18 As shown in Fig.…”

Section: Scanning Electron Microscopy and Energy Dispersive Spectroscopymentioning

confidence: 99%

Effect of duck egg white peptide‐ferrous chelate on iron bioavailability in vivo and structure characterization

Shi

et al. 2018

J Sci Food Agric

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“…These results indicated that hydrogen bonds in the collagen peptides were decreased after chelation, thus both NH and CN groups were involved in the coordination of Ca and peptides. It was suggested that COOH, OH, NH 2 as well CONH might participate in the coordination with Ca based on results of FTIR absorption peak changes of cucumber seed peptide …”

Section: Resultsmentioning

confidence: 99%

Preparation and bioavailability of calcium‐chelating peptide complex from tilapia skin hydrolysates

et al. 2017

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“…In fact, the detrimental effect of lansoprazole on sperm function was found to be, at least in part, attributable to the calcium chelating effect of lansoprazole (Banihani & Khasawneh, 2018); given that, free calcium has been approved to have a crucial function in human sperm motility (Abbasihormozi et al, 2017; Miller et al, 2016). Mechanistically, it has been recognised that chemical compounds containing at least two of the following chemical functional groups: –COOH, –OH, –SH, –S–, C=O, S=O, – N =, –NH 2 , –NH– and –O– are acknowledged to have, most of time, a metal‐chelating property (Cherrak et al, 2016; Wang et al, 2017). Such chemical property, in actual fact, applies on lansoprazole as it contains four of the mentioned organic functional groups (–NH–, –O–, –N= and S=O) (Banihani & Khasawneh, 2018).…”

Section: Discussionmentioning

confidence: 99%

Effect of lansoprazole on DNA integrity of human spermatozoa and activity of seminal creatine kinase

Banihani

Khasawneh

2020

Andrologia

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Although lansoprazole (brand name Prevacid) is a commonly used dug to manage various acid-related gastrointestinal diseases, little is known about its effects on human semen quality and sperm parameters. Here, we aimed to investigate the effect of lansoprazole on DNA integrity of human spermatozoa and activity of seminal creatine kinase. DNA integrity of human spermatozoa was assessed by the Apo-Direct™ kit followed by flow cytometry. The activity of creatine kinase was measured by kinetic spectrophotometric method using commercially available kits following the International Federation of Clinical Chemistry recommendations. Lansoprazole at 3 µg/ml, after 1-hr incubation period, did not show any significant increase in fluorescein isothiocyanate fluorescence (p > .05) and hence on the content of DNA breaks of human spermatozoa. In addition, there was no significant change (p = .8113) in the activity of seminal creatine kinase by the effect of lansoprazole. In conclusion, lansoprazole at 3 µg/ml did not alter DNA integrity of human spermatozoa or activity of seminal creatine kinase after 1-hr incubation period. K E Y W O R D Screatine kinase, DNA integrity, human semen, lansoprazole, spermatozoa

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Preparation of cucumber seed peptide-calcium chelate by liquid state fermentation and its characterization

Cited by 119 publications

References 39 publications

Effect of duck egg white peptide‐ferrous chelate on iron bioavailability in vivo and structure characterization

Effect of duck egg white peptide‐ferrous chelate on iron bioavailability in vivo and structure characterization

Preparation and bioavailability of calcium‐chelating peptide complex from tilapia skin hydrolysates

Effect of lansoprazole on DNA integrity of human spermatozoa and activity of seminal creatine kinase

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