Preparation of decellularized and crosslinked artery patch for vascular tissue-engineering application

Zhao, Yilin; Zhang, Zhigang; Wang, Jinling; Yin, Ping; Wang, Yu; Yin, Zhenyu; Zhou, Jianyin; Xu, Gang; liu, Yun; Deng, Zhigang; Zhen, Maochuan; Cui, Wugeng; Liu, Zhongchen

doi:10.1007/s10856-011-4326-3

Cited by 13 publications

(9 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, use of the 3 agents was effective in AF decellularization. Previously, decellularization with Triton X-100 completely removed nuclear material in nerve, pericardium and bone [11], [16]–[17]; with SDS removed cells in meniscus, cornea and cartilage bone [12], [14], [18]; and with trypsin removed cells in dermal, aortic and aortic valve tissue [13], [15], [19], [21]. However, the cell removal efficacy of Triton X-100 is controversial: nuclear material was observed in tendon, artery, and ligament after decellularization with Triton X-100 solution [26]–[28].…”

Section: Discussionmentioning

confidence: 99%

“…Pig AF were incubated under continuous shaking in trypsin/EDTA (0.5% trypsin and 0.2% EDTA; both Sigma) in hypotonic Tris-HCl buffer, together with RNase A (20 µg/ml) and DNase I (0.2 mg/ml) at 37°C for 72 h. The trypsin/EDTA solution was changed every 24 h. Then decellularized AF was washed with PBS for 24 h under shaking for removal of residual substances [19]–[21].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of Decellularization Protocols for Preparing a Decellularized Porcine Annulus Fibrosus Scaffold

et al. 2014

View full text Add to dashboard Cite

Tissue-specific extracellular matrix plays an important role in promoting tissue regeneration and repair. We hypothesized that decellularized annular fibrosus matrix may be an appropriate scaffold for annular fibrosus tissue engineering. We aimed to determine the optimal decellularization method suitable for annular fibrosus. Annular fibrosus tissue was treated with 3 different protocols with Triton X-100, sodium dodecyl sulfate (SDS) and trypsin. After the decellularization process, we examined cell removal and preservation of the matrix components, microstructure and mechanical function with the treatments to determine which method is more efficient. All 3 protocols achieved decellularization; however, SDS or trypsin disturbed the structure of the annular fibrosus. All protocols maintained collagen content, but glycosaminoglycan content was lost to different degrees, with the highest content with TritonX-100 treatment. Furthermore, SDS decreased the tensile mechanical property of annular fibrosus as compared with the other 2 protocols. MTT assay revealed that the decellularized annular fibrosus was not cytotoxic. Annular fibrosus cells seeded into the scaffold showed good viability. The Triton X-100–treated annular fibrosus retained major extracellular matrix components after thorough cell removal and preserved the concentric lamellar structure and tensile mechanical properties. As well, it possessed favorable biocompatibility, so it may be a suitable candidate as a scaffold for annular fibrosus tissue engineering.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Comparison of Decellularization Protocols for Preparing a Decellularized Porcine Annulus Fibrosus Scaffold

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Notably, there is no absolute method for cartilage sterilization and decellularization. Common methods often include chemicals (e.g., NaOH, H 2 O 2 ) and/or enzymes (e.g., DNase/RNase, pepsin) . Recently, Kang et al reported that 1% Triton X‐100 successfully reduced the DNA content in human nasal septal cartilage, indicating that this protocol may be ideal for the sterilization and decellularization of other types of cartilage .…”

Section: Discussionmentioning

confidence: 99%

“…Common methods often include chemicals (e.g., NaOH, H 2 O 2 ) and/or enzymes (e.g., DNase/ RNase, pepsin). 26,27 Recently, Kang et al reported that 1% Triton X-100 successfully reduced the DNA content in human nasal septal cartilage, indicating that this protocol may be ideal for the sterilization and decellularization of other types of cartilage. 17 Here, we found that 1% Triton X-100-treated porcine auricular cartilage did not express MHC classes I and II antigens and the DNA content was reduced in 99.8% of the samples.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of decellularized xenogenic porcine auricular cartilage as a novel biocompatible filler

et al. 2018

View full text Add to dashboard Cite

Fillers are products that fill the space in soft tissues of the human body and actively used in the various medical fields. Unfortunately, most of the cost-effective commercially available fillers are synthetic and have limitations in terms of their biocompatibility. Here, we evaluated the possible application of decellularized xenogenic cartilage as a long-lasting material for soft tissue augmentation and compared it with two commercially available fillers Artesense (polymethylmethacrylate microspheres) and Radiesse (calcium hydroxyapatite [CaHa]). To do so, porcine auricular cartilage was harvested, followed by freezing and grinding of the tissue into flakes. Then, we used 1% Triton X-100 to decellularize the flakes. We then, respectively, injected 0.1 cc of each material (decellularized xenogenic cartilage, Radiesse, and Artesense) into the subcutaneous layer at three different sites per subject in 12 Sprague-Dawley rats, and evaluated the inflammatory cell infiltration and foreign body reactions of each. Our data indicate that the infiltration of giant cells in the injection area was significantly lower in the decellularized xenogenic cartilage injection group than that in the Radiesse and Artesense injection groups. Further, we observed some neutrophil infiltration in the xenogenic cartilage and Artesense injection groups at 1 month, but these levels were much lower at 3 months (comparable to the Radiesse injection group). Thus, decellularized xenogenic cartilage may have a distinct advantage in terms of biocompatibility compared with other commercial injectable long-lasting fillers, making it one of the most feasible, natural, and cost effective materials in the market. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2708-2715, 2018.

show abstract

“…Decellularized scaffold is prepared by eliminating the cellular components from the biological tissue and leaving the extracellular matrix scaffold, which can be used in artificial organ and tissue regeneration . Previous reports have shown that decellularized scaffold has been widely used to engineer vascular graft ; nevertheless, it has not been used to engineer lymphatic graft yet.…”

Section: Introductionmentioning

confidence: 99%

Construction of tissue‐engineered lymphatic vessel using human adipose derived stem cells differentiated lymphatic endothelial like cells and decellularized arterial scaffold: A preliminary study

Yang

Chen

et al. 2017

Biotech and App Biochem

View full text Add to dashboard Cite

We have previously demonstrated that human adipose-derived stem cells (hADSCs) can be differentiated into lymphatic endothelial like cells. The purpose of this study was to investigate the feasibility of utilizing the induced lymphatic endothelial like cells and decellularized arterial scaffold to construct the tissue-engineered lymphatic vessel. The hADSCs were isolated from adipose tissue in healthy adults and were characterized the multilineage differentiation potential. Decellularized arterial scaffold was prepared using the Triton x-100 method. ADSCs were differentiated into lymphatic-like endothelial cells, and the induced cells were then seeded onto the decellularized arterial scaffold to engineer the lymphatic vessel. The histological analyses were performed to examine the endothelialized construct. The decellularized arterial scaffold was successfully obtained and was able to maintain its vessel morphology. The isolated ADSCs can be differentiated into osteocytes and adipocytes. After seeding onto the scaffold, the seeded cells attached and grew well on the decellularized arterial scaffold. Our preliminary results demonstrated that the induced lymphatic endothelial like cells combined with decellularized arterial scaffold could be utilized to successfully engineer the lymphatic vessel. Our findings may be helpful for the development of tissue-engineering of the lymphatic graft.

show abstract

Preparation of decellularized and crosslinked artery patch for vascular tissue-engineering application

Cited by 13 publications

References 30 publications

Comparison of Decellularization Protocols for Preparing a Decellularized Porcine Annulus Fibrosus Scaffold

Comparison of Decellularization Protocols for Preparing a Decellularized Porcine Annulus Fibrosus Scaffold

Evaluation of decellularized xenogenic porcine auricular cartilage as a novel biocompatible filler

Construction of tissue‐engineered lymphatic vessel using human adipose derived stem cells differentiated lymphatic endothelial like cells and decellularized arterial scaffold: A preliminary study

Contact Info

Product

Resources

About