Preparation of granulocyte concentrates by apheresis: collection modalities in the USA

Strauss, Ronald G.; Hg, Klein; Sf, Leitman; Price, Thomas H.; Lichtiger, Benjamin; Martínez, Francisco García; Reesink, H. W.; Panzer, Simon

doi:10.1111/j.1423-0410.2010.01417.x

Cited by 14 publications

(18 citation statements)

References 14 publications

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“…However, some blood centres do not respect AB0 Rh compatibility, though incompatible red cells are removed from the granulocyte component in these cases 4 . Granulocyte donors were not routinely screened for HLA antibodies in this study, and a different approach was used for routine HLA screening 5,6 .…”

Section: Discussionmentioning

confidence: 99%

Procedure for granulocyte collection performed at the Blood Centre of the Faculty Hospital Ostrava

Čermáková

Blahutová²,

Papajík³

et al. 2014

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Background. Granulocyte apheresis is a safe and effective method for granulocyte collection. We present a five year experience (2006)(2007)(2008)(2009)(2010) of the Blood Center, Faculty Hospital Ostrava, Czech Republic. Donor granulocyte transfusion is one treatment option for haemato-oncology patients with severe neutropenia complicated by bacterial/fungal infections unresponsive to standard antibiotic/antifungal treatment. In this study, we describe the experiences of the Blood Centre at the Faculty Hospital in Ostrava of granulocyte apheresis. Methods and Results. A total of 149 granulocyte units were collected for 33 patients from the Department of Haematooncology, University Hospital Olomouc, over a 5-year period (2006)(2007)(2008)(2009)(2010). Information on donor selection, laboratory screening, mobilization regimen and granulocyte yield was obtained and analyzed. All mandatory screening tests for infection markers, AB0 and Rh and abnormal erythrocyte antibodies were performed. The cytomegalovirus status of the donors was not investigated. Steroids were the only mobilization regimen used, and were generally well accepted. The mean granulocyte yield was 12.6×10 9 /per transfusion unit (range 5.4-30.3). All granulocyte concentrates were irradiated and transfused according to AB0 Rh compatibility within 24 h after collection. Conclusion. Based on our experience, granulocytapheresis is a safe and effective method for obtaining granulocytes but the yield can be significantly influenced by other variables. From the recipients' perspective, the use of donor granulocytes supports an effective therapeutic modality.

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Section: Discussionmentioning

confidence: 99%

Procedure for granulocyte collection performed at the Blood Centre of the Faculty Hospital Ostrava

Čermáková

Blahutová²,

Papajík³

et al. 2014

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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“…The addition of dexamethasone to G-CSF was shown to increase the yield even more (by a factor of 1.5x) [17]. Since then, the approach in many U.S. centers has been to use the combination of G-CSF and dexamethasone [18]. This approach is not universal, however, and several centers in Europe do not use G-CSF [19].…”

Section: Brief History Of Granulocyte Transfusionsmentioning

confidence: 99%

“…This approach is not universal, however, and several centers in Europe do not use G-CSF [19]. Overall, there seem to be significant technical differences from center to center nationally and internationally [18,19] and these could be a persistent source of differences in observed outcomes.…”

Section: Brief History Of Granulocyte Transfusionsmentioning

confidence: 99%

“…Although these antibodies have shown to be associated with diminishing increment in the ANC and shorter duration of detectable neutrophil counts in animal models [71], an early report on 187 GTX transfusions to 19 neutropenic cancer patients failed to identify a statistically significant correlation between the presence of antibodies and the response to the transfusions [72], and testing for the presence of these antibodies is not part of the routine procedure at most institutions [18,19]. Alloimmuniization to HLA and neutrophil seems to occur very frequently (depending on how many GTX are administered).…”

Section: Toxicity Of Granulocyte Transfusionsmentioning

confidence: 99%

“…The surveys on GTX practices from Europe [19] and the US [18] Show that most (but not all) use CMV negative donors preferentially, if not exclusively, at least for CMV-negative recipients. This was also the practice in the RING trial.…”

Section: Toxicity Of Granulocyte Transfusionsmentioning

confidence: 99%

See 2 more Smart Citations

Granulocyte transfusions: A concise review for practitioners

Gea-Banacloche

2017

Cytotherapy

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Granulocyte transfusions (GTXs) have been used to treat and prevent infections in neutropenic patients for more than 40 years, despite persistent controversy regarding their efficacy. This narrative review attempts to complement recent systematic reviews by the Cochrane Collaboration and provide both historical context and critical assessment of the most significant clinical studies published over the years. The data suggest that properly collected and promptly infused granulocytes are active against infections, both bacterial and fungal. The most important question that remains unanswered is in which patients the administration of granulocytes will be beneficial. The preponderance of evidence suggests that granulocyte transfusions may be efficacious in few select cases as a temporizing measure to control an infection that is expected (or proven) to be refractory to optimal antimicrobial treatment, and that could otherwise be controlled by marrow recovery, which is expected to happen. In this regard, they are best considered a “bridge” that grants enough time for the recipient to develop their own response to the infection. The challenges to use GTXs successfully are both clinical, in terms of timely identifying the patients who may benefit, and logistical, in terms of optimal selection of donors and collection technique.

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Granulocyte collection by polymorphonuclear cell-targeting apheresis with medium-molecular-weight hydroxyethyl starch

et al. 2021

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Preparation of granulocyte concentrates by apheresis: collection modalities in the USA

Cited by 14 publications

References 14 publications

Procedure for granulocyte collection performed at the Blood Centre of the Faculty Hospital Ostrava

Procedure for granulocyte collection performed at the Blood Centre of the Faculty Hospital Ostrava

Granulocyte transfusions: A concise review for practitioners

Granulocyte collection by polymorphonuclear cell-targeting apheresis with medium-molecular-weight hydroxyethyl starch

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