Preparation of mesoporous activated carbon from defective coffee beans for adsorption of fresh whey proteins

Batista, Giovanni Aleixo; Silva, Maria Letícia Martins; Gomes, Willian de Paula; Neves, Isabelle Cristina Oliveira; Mól, Paula Chequer Gouveia; Resende, Jaime Vilela de; Veríssimo, Lizzy Ayra Alcântara; Ribeiro-Soares, J.

doi:10.4025/actascitechnol.v42i1.45914

Cited by 2 publications

(1 citation statement)

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“…Andrade et al [123] investigated the potential of coconut endocarp as the carbon precursor material for the synthesis of the activated carbon as an adsorbent for whey protein purification, activated by ZnCl 2 . Batista et al [124] developed a mesoporous activated carbon from defective coffee beans for the adsorption of fresh whey protein, using activation by phosphoric acid. Adsorption properties od BSA for different activated carbons, such as thermally expanded graphite and graphene nanoplatelets were also investigated [125].…”

Section: Activated Carbonmentioning

confidence: 99%

Application of Ion Exchange and Adsorption Techniques for Separation of Whey Proteins from Bovine Milk

Radosavljević

Stanić-Vučinić

Stojadinovic

et al. 2021

CAC

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Background: The world production of whey was estimated to be more than 200 million tons per year. Although whey is an important source of proteins with high nutritional value and biotechnological importance, it is still considered as a by-product of the dairy industry with low economic value due to low industrial exploitation. There are several challenges in the separation of whey proteins: low concentration, the complexity of the material and similar properties (pI, molecular mass) of some proteins. Methods: A narrative review of all the relevant papers on the present methodologies based on ion-exchange and adsorption principles for isolation of whey proteins, known to the authors, was conducted. Results: Traditional ion-exchange techniques are widely used for the separation and purification of the bovine whey proteins. These methodologies, based on the anion or cation chromatographic procedures, as well as combination of aforementioned techniques are still preferential methods for the isolation of the whey proteins on the laboratory scale. However, more recent research on ion exchange membranes for this purpose has been introduced, with promising potential to be applied on the pilot industrial scale. Newly developed methodologies based either on the ion-exchange separation (for example: simulated moving bed chromatography, expanded bed adsorption, magnetic ion exchangers, etc.) or adsorption (for example: adsorption on hydroxyapatite or activated carbon, or molecular imprinting) are promising approaches for scaling up of the whey proteins’ purification processes. Conclusion: Many procedures based on ion exchange are successfully implemented for separation and purification of whey proteins, providing protein preparations of moderate-to-high yield and satisfactory purity. However, the authors anticipate further development of adsorption-based methodologies for separation of whey proteins by targeting the differences in proteins’ structures rather than targeting the differences in molecular masses and pI. The complex composite multilayered matrices, including also inorganic components, are promising materials for simultaneous exploiting of the differences in the masses, pI and structures of whey proteins for the separation.

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Section: Activated Carbonmentioning

confidence: 99%