Preparation of Poloxamer188-b-PCL and Study on in vitro Radioprotection Activity of Curcumin-Loaded Nanoparticles

Abstract

A novel polymer of poloxamer188-b-PCL was synthesized via a ring-opening polymerization. Fourier transform infrared spectroscopy (FTIR), Raman, and 1 H nuclear magnetic resonance (1 H NMR) spectra were used to study the structures of obtained poloxamer188-b-PCL. The thermo-stability of poloxamer188-b-PCL was carried out with a thermal gravimetric analyzer (TGA), and cytotoxicity was obtained using the CCK8 method. Cargo-free and curcumin (CUR)-loaded poloxamer188-b-PCL NPs were fabricated via the solvent evaporation method. The morphology, particle size distribution, and stability of cargo-free NPs were studied with a scanning electron microscope (SEM) and laser particle analyzer. The in vitro radioprotection activity of CUR-loaded NPs was performed. FTIR, Raman, and 1 H NMR spectra confirmed that poloxamer188-b-PCL was obtained. TGA curves suggested poloxamer188-b-PCL had better thermo-stability than original poloxamer188. Cell tests suggested that the cargo-free NPs had no cytotoxicity. SEM image showed that the cargo-free NPs were spherical with a diameter of 100 nm. Free radical scavenging experiments proved that CUR-loaded NPs had better antioxidant activity than CUR solutions. CUR-loaded NPs could be detected in all tissues, including liver, kidneys and lung. In summary, this work demonstrated a feasibility of developing an injective formulation of CUR and provided a protection agent in caner radiotherapy.

Overcoming MDR by Associating Doxorubicin and pH-Sensitive PLGA Nanoparticles Containing a Novel Organoselenium Compound—An In Vitro Study

“…This same tendency was observed in ABTS assay, in which the ACAT-Se-PLGA-77KL-NPs presented superior radical scavenging activity in comparison to free ACAT-Se. It is worth mentioning that the increase of the antioxidant activity by the nanoencapsulation was also reported in other studies [57][58][59][60]. Some authors suggest that the increase of this activity could be attributed to the superior contact surface area between the H donator and DPPH or ABTS molecules provided by the nanometric size of the particles, which thus offers easier access of the hydrogen atom to the radical site [58,60].…”

Development of nanotechnology-mediated precision radiotherapy for anti-metastasis and radioprotection

“…[722][723][724] Various organic nanocarriers have been used to deliver small molecular radioprotectors, such as WR-1065, curcumin, g-tocotrienol and baicalein, to specific organs or tissues for higher accumulation and longer retention time. [725][726][727][728][729][730][731][732][733][734][735][736][737][738][739] Gastrointestinal tract, one of the organs most vulnerable to radiation, can progress to severe gastrointestinal syndrome under radiation at a dose higher than 8-10 Gy. 740,741 Thus, radioprotection of the gastrointestinal tract is urgent and important.…”

Budesonide Compatibility Study With Excipients for Preparation of Nanoparticle

“…Poloxamer 188, a poly (ethylene oxide) (PEO) and poly (propylene oxide) (PPO) triblock copolymer, defends cell membranes against various stresses. Amphiphilic block copolymers are made up of hydrophilic and hydrophobic blocks covalently bonded together [11,12]. Poloxamers have been used as functional biomaterials for the treatment of cancer, such as drug delivery vectors for heat-sensitive hydrogels and chemosensitizing agents.…”