Preparation of stable cross-linked enzyme aggregates (CLEAs) of NADH-dependent nitrate reductase and its use for silver nanoparticle synthesis from silver nitrate

Talekar, Sachin; Joshi, Gandhali; Chougle, Radhika; Nainegali, Basavaraj S.; Desai, Shashikant; Joshi, Asavari; Kambale, Shashikant; Kamat, Priyanka; Haripurkar, Rutumbara; Jadhav, Suraj; Nadar, Shamraja S.

doi:10.1016/j.catcom.2014.05.003

Cited by 45 publications

(19 citation statements)

References 20 publications

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“…Ahmad et al 66 subsequently demonstrated the use of single-spore bacteria (Thermomonospora sp.) 67 55 Au NPs B15 nm Cytoplasmic proteins L. edodes 71 Au NPs 5-50 nm Laccase, tyrosinase, and Mn-peroxidase F. oxysporum 72 Ag NPs 10-20 nm Nitrate reductase Thermomonospora sp. The results pointed to proteins (molecular weight 80-10 000) playing an important role in the reduction of Au(III) ions at 50 1C, also favorable for the survival of the thermophilic bacteria.…”

Section: Microorganism-mediated Synthesismentioning

confidence: 99%

Bio-inspired synthesis of metal nanomaterials and applications

et al. 2015

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Section: Microorganism-mediated Synthesismentioning

confidence: 99%

Bio-inspired synthesis of metal nanomaterials and applications

et al. 2015

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“…(b)). However, the activity recovery decreased beyond 1.5 h. This decreased rate of cross‐linking might be due to an aldol condensation of free glutaraldehyde molecules with glutaraldehyde molecules still grafted onto the enzyme . This result implied that prolonged cross‐linking time results in rigidification of the enzyme due to a more intensive cross‐linking which restricts enzyme flexibility and reduces enzyme activity …”

Section: Resultsmentioning

confidence: 99%

Preparation of spherical cross-linked lipase aggregates with improved activity, stability and reusability characteristic in water-in-ionic liquid microemulsion

Cui

Lin

Feng

et al. 2017

J. Chem. Technol. Biotechnol

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BACKGROUND Cross‐linking enzyme aggregates (CLEAs) seem to be a promising immobilization method due to mild processing conditions and short preparation time. However, the CLEAs prepared in aqueous solution exhibit amorphous large clusters that cause significant mass‐transfer limitations, leading to low catalytic efficiency. In this work, novel spherical CLEAs of lipase from bovine pancreas (spherical CLEAs) were prepared within droplets dispersed in a water‐in‐hydrophobic ionic liquid microemulsion rather than a conventional aqueous solution. The properties of the spherical CLEAs were determined. RESULTS The resultant CLEAs exhibited spherical structure with good monodispersity instead of the amorphous clusters of conventional CLEAs, and showed higher activity recovery (84.6%) than that of conventional CLEAs (52.8%). The high activity recovery of spherical CLEAs could be attributed to both interfacial activation of the microemulsion system and the well‐defined structure of the spherical CLEAs, which not only activated lipases in an active form, but also decreased mass‐transfer limitations. Moreover, the thermostability, storage stability, tolerance against hydrophilic solvents, and mechanical stability of the spherical CLEAs were enhanced significantly. The spherical CLEAs evidenced excellent reusability in comparison with conventional CLEAs. CONCLUSIONS The novel strategy in this study might open an attractive way towards effectively improving the catalytic properties of traditional CLEAs. © 2016 Society of Chemical Industry

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“…(45) α-NADPH-dependent nitrate reductase isolated from the fungus Fusarium oxysporum was able to produce AgNPs in a reaction which required 4-hydroxyquinoline as an electron shuttle, as shown in Figure 2a. (44,(46)(47)(48) The requirement of an electron shuttle indicated that the enzyme was not directly reducing the Ag + and the NO3in parallel, but instead reduced the nitrate and the generated electron was transferred to the Ag + which was subsequently reduced in a series fashion. Additionally, the electrochemistry of the involved species favoured the electron transfer to Ag + from NO3 -, as can be seen in Figure 2b.…”

Section: Mechanisms Of Silver Nanoparticle Biosynthesismentioning

confidence: 99%

Bacteria and nanosilver: the quest for optimal production

Mabey

Cristaldi

Oyston

et al. 2019

Critical Reviews in Biotechnology

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Silver nanoparticles (AgNPs) have potential uses in many applications, but current chemical production methods are challenged by scalability, limited particle stability, and the use of hazardous chemicals. The biological processes present in bacteria to mitigate metallic contaminants in their environment present a potential solution to these challenges. Before commercial exploitation of this technology can be achieved, the quality of bacteriogenic AgNPs needs to be improved for certain applications. While the colloidal and morphological stabilities of biogenic AgNPs are widely regarded as superior to chemogenic particles, little control over the synthesis of particle morphologies has been achieved in biological systems. This article reviews a range of biosynthetic reaction conditions and how they affect AgNP formation in bacteria to understand which are most influential. While there remains uncertainty, some general trends are emerging: higher Ag + concentrations result in higher AgNP production, up to a point at which the toxic effects begin to dominate; the optimal temperature appears to be heavily species-dependent and linked to the optimal growth temperature of the organism. However, hotter conditions generally favour higher production rates, while colder environments typically give greater shape diversity. Little attention has been paid to other potentially important growth conditions including halide concentrations, oxygen exposure, and irradiation with light. To fully exploit biosynthetic production routes as alternatives to chemical methods, hurdles remain with controlling particle morphologies and require further work to elucidate and harness. By better understanding the factors influencing AgNP production a foundation can be laid from which shape-controlled production can be achieved.

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Preparation of stable cross-linked enzyme aggregates (CLEAs) of NADH-dependent nitrate reductase and its use for silver nanoparticle synthesis from silver nitrate

Cited by 45 publications

References 20 publications

Bio-inspired synthesis of metal nanomaterials and applications

Bio-inspired synthesis of metal nanomaterials and applications

Preparation of spherical cross-linked lipase aggregates with improved activity, stability and reusability characteristic in water-in-ionic liquid microemulsion

Bacteria and nanosilver: the quest for optimal production

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