Preparative isolation and purification of three flavonoids from the Chinese medicinal plant Epimedium koreamum Nakai by high-speed counter-current chromatography

Liu, Renmin; Li, Aifeng; Sun, Ailing; Cui, Jichun; Kong, Ling–Yi

doi:10.1016/j.chroma.2004.12.026

Cited by 69 publications

(37 citation statements)

References 3 publications

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“…This method has been recently applied to isolation and purifi cation of bioactive compounds from plants, microbial origins, and marine organisms, providing a number of useful references. 17,18 CPC was fi rst used by Sanki Eng in 1982. A CPC column is composed of a series of channels linked in cascade by ducts and aligned in cartridges or disks in a circle around a rotor; setting the rotor in motion submits this assembly to a constant centrifugal fi eld that keeps the stationary phase in place.…”

Section: Droplet Countercurrent (Dcc) and High -Speed Countercurrent mentioning

confidence: 99%

Extraction and Isolation of Compounds from Herbal Medicines

Liu

2010

Traditional Herbal Medicine Research Methods

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Section: Droplet Countercurrent (Dcc) and High -Speed Countercurrent mentioning

confidence: 99%

Extraction and Isolation of Compounds from Herbal Medicines

Liu

2010

Traditional Herbal Medicine Research Methods

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“…Flavonoids of Epimedii herba can be detected by various analytical methods, including CZE [5], HPLC [6,7], high-speed counter-current chromatography [7], and high performance TLC (HPTLC) [8]. The purpose of this work is to develop a reliable HPTLC densitometric procedure for the rapid identification and quantitation of icariin in dry extracts of E. koreanum and to compare the results with the established HPLC method.…”

Section: Introductionmentioning

confidence: 99%

Comparison between HPLC and HPTLC densitometry for the determination of icariin from Epimedium koreanum extracts

Pozharitskaya¹,

Косман²,

Shikov³

et al. 2007

J of Separation Science

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Dry extracts of the aerial parts of Epimedium koreanum were quantified by HPLC and high performance TLC (HPTLC). A gradient HPLC method was used for the quantification of the prenylflavone glycoside icariin at 270 nm. A direct HPTLC assay was developed for the determination of icariin at 270 nm. The UV detection of both analytical assays were used to examine the purity of icariin peaks and compared with the standards. The assays provide good accuracy, reproducibility, and selectivity for the quantitative analysis of icariin. The icariin contents of five different dry extracts were compared by HPLC and HPTLC densitometry. The quantitative results of both analytical methods did not show any statistically significant differences between them, although a trend to slightly lower mean values could be found for the HPLC method.

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“…1) is one of the major flavonoid glycosides isolated from Herba Epimedium (HEP, family Berberidaceae), which has been used as a traditional Chinese medicine (TCM) to treat osteoporosis, coronary artery disease and male sexual dysfunction for over 2,000 years [1][2][3]. The amount of natural ICS II that could be extracted is as much as 9% (w/w) of total flavonoids from Epimedium koreamum Nakai [4]. Modern pharmacological studies demonstrate that ICS II has many biological activities, including anti-osteoporosis, anti-hypoxia and anti-cancer activities [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

LC Determination of Icariside II in Rat Plasma and Tissues: Application to a Tissue Distribution Study

et al. 2011

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A sensitive and reliable reversed-phase liquid chromatography (RP-LC) with ultraviolet (UV) detection has been developed and validated for the quantification of Icariside II in rat plasma and tissues using Fermononetin as the internal standard. Protein precipitation and liquidliquid extraction were utilized for plasma and tissue sample preparation, respectively. The analysis was successfully carried out on an Agilent SB-C 18 column (5 lm, 4.6 9 250 mm) with the implementation of the following conditions: a mobile phase of phosphoric acid solution (0.1%, v/w)-Acetonitrile (55:45, v/v), a flow rate of 1 mL min -1 , a column temperature of 25°C and a detection wavelength of 270 nm. Good linear relationships of calibration curves were obtained (r 2 [ 0.9906) over the investigated concentration range with plasma and tissue samples. The lower limit of quantification (LLOQ) and the limit of detection (LOD) were 0.1 and 0.02 lg g -1 , respectively (for plasma sample, they were 0.05 and 0.1 lg mL -1 , respectively). The developed method which was embodied with good precision, accuracy, recovery and stability was corroborated to satisfy the requirements for biomedical sample analysis. This method has been successfully applied to tissue distribution study of Icariside II in rats after a single intravenous dose at 12.5 mg kg -1 . Results suggested that Icariside II was distributed to rat tissues rapidly with greater initial concentrations in kidney, lung and liver. Moderate initial distributions were obtained in rat muscle, heart, bone, spleen and plasma. Low amount of Icariside II was detected in testes, and no Icariside II could be detected in the brain.

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Preparative isolation and purification of three flavonoids from the Chinese medicinal plant Epimedium koreamum Nakai by high-speed counter-current chromatography

Cited by 69 publications

References 3 publications

Extraction and Isolation of Compounds from Herbal Medicines

Extraction and Isolation of Compounds from Herbal Medicines

Comparison between HPLC and HPTLC densitometry for the determination of icariin from Epimedium koreanum extracts

LC Determination of Icariside II in Rat Plasma and Tissues: Application to a Tissue Distribution Study

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