Presence of a generalist entomopathogenic fungus influences the oviposition behaviour of an aphid-specific predator

Azevedo, A. G. C. de; Stuart, Robyn M.; Sigsgaard, Lene

doi:10.1007/s10526-018-9889-1

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…Since then, studies have aimed to close this gap. Tests on soil-dwelling non-target arthropods have been conducted in the laboratory (de Azevedo et al 2019;Saito and Brownbridge 2016), in the field (Babendreier et al 2015;Fischhoff et al 2017), and under semi-natural conditions (de Azevedo et al 2018). However, studies vary in product formulation, concentration, exposure method, and length as well as endpoints and quality control, complicating their comparison and thus subsequent risk evaluation.…”

Section: Discussionmentioning

confidence: 99%

Standard non-target tests for risk assessment of plant protection products are unsuitable for entomopathogenic fungi—a proposal for a new protocol

et al. 2021

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Purpose The successful implementation of a plant protection product depends on its effectiveness against a target species and its safety for the environment. Risk assessment schemes have therefore been devised to facilitate classification and regulation. These guidelines, however, are directed towards chemical substances and are in many cases less suitable for the assessment of products employing microorganisms. Methods In this study, we developed a protocol for non-target testing of soil-applied entomopathogenic fungi for the biocontrol of insect pests. Using the predatory mite Gaeolaelaps (Hypoaspis) aculeifer as a non-target model organism, our protocol evaluates the lethal and sublethal effects of the fungus in recommended and ten-fold field concentrations. Results The proposed protocol considers fungal biology when setting test duration, endpoints, and quality control measures. To assess its practicability, we performed a trial with Metarhizium brunneum ART2825 as a representative entomopathogenic fungus. The biocontrol agent was able to infect a susceptible host and reproduce, showing that potential hazards can be detected using our approach. No hazard was detected for the non-target species, with no statistically significant differences in 5-week survival and reproductive output between treated and untreated groups. Conclusion Based on our results, the protocol is deemed appropriate for the detection of non-target effects. Subject to further validation, our approach could thus provide the basis for standardized protocols for the evaluation of the environmental safety of biocontrol organisms.

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Section: Discussionmentioning

confidence: 99%

Standard non-target tests for risk assessment of plant protection products are unsuitable for entomopathogenic fungi—a proposal for a new protocol

et al. 2021

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“…However, certain MBCAs may have a broader spectrum with a potential risk of side effects against non-target organisms related to the targeted organisms, e.g. the generalist entomopathogenic fungus Metarhizium brunneum can influence the behaviour of a predator of aphids (de Azevedo et al 2018). MBCAs are typically isolated from environments similar to environments where they are applied.…”

Section: Synthesis: System Dynamics Population Densities and Risksmentioning

confidence: 99%

Ecological arguments to reconsider data requirements regarding the environmental fate of microbial biocontrol agents in the registration procedure in the European Union

Köhl

Booij

Kolnaar³

et al. 2019

BioControl

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“…The Aphidoletes aphidimyza Rondani (Diptera: Cecidomyiidae) is widely utilized as an efficient predator of aphids in various agricultural systems, and its larvae can prey voraciously on more than 80 aphid species (Boulanger et al, 2019). To date studies on A. aphidimyza have focused on biological characteristics (Choi et al, 2004; OPEN ACCESS EDITED BY Zhijun Yu, Hebei Normal University, China Guo et al, 2014;Watanabe et al, 2016;De Azevedo et al, 2018;Madahi et al, 2019;Fratoni et al, 2020;Higashida et al, 2022), biological control (Lin et al, 2017;Shang et al, 2019), and the mitochondrial genome (Shen et al, 2022), but little is known about molecular mechanisms. Gene expression-level analysis is fundamental in the study of regulatory mechanisms of genes related to host selection, predation, drug resistance, stagnation, and neuromodulation.…”

Section: Introductionmentioning

confidence: 99%

Selection and validation of optimal reference genes for RT-qPCR analyses in Aphidoletes aphidimyza Rondani (Diptera: Cecidomyiidae)

Shen,

Zhang,

Zhao

et al. 2023

Front. Physiol.

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Aphidoletes aphidimyza is a predator that is an important biological agent used to control agricultural and forestry aphids. Although many studies have investigated its biological and ecological characteristics, few molecular studies have been reported. The current study was performed to identify suitable reference genes to facilitate future gene expression and function analyses via quantitative reverse transcription PCR. Eight reference genes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), RPS13, RPL8, RPS3, α-Tub, β-actin, RPL32, and elongation factor 1 alpha (EF1-α) were selected. Their expression levels were determined under four different experimental conditions (developmental stages, adult tissues, sugar treatment, and starvation treatment) using qRT-PCR technology. The stability was evaluated with five methods (Ct value, geNorm, NormFinder, BestKeeper, and RefFinder). The results showed that GAPDH, RPL32, and EF1-α were ranked as the best reference gene combinations for measuring gene expression levels among different developing stages and in various starvation treatments. RPL8 and RPS3 were recommended to normalize the gene expression levels among different adult tissues. RPL32, β-actin, and EF1-α were recommended sugar-feeding conditions. To validate the utility of the selected reference pair, RPL8, and RPS3, we estimated the tissue-biased expression level of a chemosensory protein gene (AaphCSP1). As expected, AaphCSP1 is highly expressed in the antennae and lowly expressed in the abdomen. These findings will lay the foundation for future research on the molecular physiology and biochemistry of A. aphidimyza.

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Presence of a generalist entomopathogenic fungus influences the oviposition behaviour of an aphid-specific predator

Cited by 4 publications

References 23 publications

Standard non-target tests for risk assessment of plant protection products are unsuitable for entomopathogenic fungi—a proposal for a new protocol

Standard non-target tests for risk assessment of plant protection products are unsuitable for entomopathogenic fungi—a proposal for a new protocol

Ecological arguments to reconsider data requirements regarding the environmental fate of microbial biocontrol agents in the registration procedure in the European Union

Selection and validation of optimal reference genes for RT-qPCR analyses in Aphidoletes aphidimyza Rondani (Diptera: Cecidomyiidae)

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