Presence of Fusarium Wilt, Incited by <i>Fusarium oxysporum</i> f.sp. <i>lactucae</i>, on Lettuce in France

Gilardi, G.; Pons, C.; Gard, Benjamin; Ortega, S. Franco; Gullino, M. L.

doi:10.1094/pdis-12-16-1815-pdn

Cited by 15 publications

(8 citation statements)

References 3 publications

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“…Typical symptoms are leaf chlorosis, vascular browning and wilting. The widespread race 1 has been reported in Japan (Fujinaga et al, 2003), the United States, Taiwan, Iran, Brazil, Argentina, Portugal, Italy and France (Gilardi et al, 2017;Gordon & Koike, 2015). In contrast, race 2 has only been found in Japan while race 3 has been reported in Taiwan and Japan (Gordon & Koike, 2015;Lin et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Fusarium isolates from Belgium causing wilt in lettuce show genetic and pathogenic diversity

Claerbout

Poucke²,

Mestdagh

et al. 2022

Plant Pathology

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Fusarium oxysporum f. sp. lactucae race 4 causes vascular necrosis and wilting of lettuce. First observed in Belgium in 2015, the lack of disease resistance in commercial cultivars allowed this pathogen to spread to nearly the entire Belgian production area within 4 years. Different levels of disease development were observed in different commercial greenhouses. To help explain this variation, we collected 78 Fusarium isolates and characterized them both physiologically and genetically. Molecular race identification indicated that 91% of the isolates belonged to race 4, while 6% of the isolates belonged to race 1, which was not previously reported in Belgium. Pathogenicity assays using differential cultivars confirmed the molecular race assignment of selected isolates. Cultivar Patriot was identified as a suitable new differential cultivar to race 4. Race 4 isolates were more aggressive than race 1 isolates at 24°C, but only when using chlamydospore inoculum instead of a root dip assay containing microconidia. Variation in pathogenicity and aggressiveness of the races may explain differences in disease development in commercial greenhouses. Based on genotyping‐by‐sequencing (GBS), race 1 and race 4 isolates were highly similar to reference isolates. Fusarium curvatum, F. oxysporum f. sp. tulipae and F. oxysporum f. sp. rhois were phylogenetically separated from F. oxysporum f. sp. lactucae races 1 and 4 based on the GBS data, but not when using multilocus sequence data. Within F. oxysporum f. sp. lactucae race 4, the GBS data differentiated two rather homogeneous groups, suggesting at least two introductions. However, the two groups did not differ in aggressiveness.

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Section: Introductionmentioning

confidence: 99%

Fusarium isolates from Belgium causing wilt in lettuce show genetic and pathogenic diversity

Claerbout

Poucke²,

Mestdagh

et al. 2022

Plant Pathology

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“…The LAMP assay developed in this study has been validated according to the international EPPO standard 7/98 to detect FOL in plant material, soil and seeds. Although there is no specific phytosanitary legislation within the EU for the detection of FOL in lettuce seeds in trade or lettuce plant material, the increased spread of the pathogen and the recent identification of a new race within FOL (Gilardi et al, 2016(Gilardi et al, , 2017 makes the development and availability of new techniques for the diagnosis of FOL essential. Garibaldi et al, (2004) demonstrated the presence of FOL in lettuce seeds, assessing 27 samples of different cultivars of commercial lettuce seeds from Lombardy (north Italy).…”

Section: Discussionmentioning

confidence: 99%

Rapid detection of Fusarium oxysporum f. sp. lactucae on soil, lettuce seeds and plants using loop‐mediated isothermal amplification

et al. 2018

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Fusarium oxysporum f. sp. lactucae (FOL) is a soil-and seedborne pathogen and the causal agent of fusarium wilt on lettuce. Four races have been identified within FOL, with different worldwide distribution. Several molecular techniques have been used to detect and identify this pathogen; however, not all of them have the optimal characteristics in terms of sensitivity to perform FOL detection in plant and seed material. A loop-mediated isothermal amplification (LAMP) assay was developed based on the sequence-characterized amplified region (SCAR) obtained in a previous rapid amplification of polymorphic DNA (RAPD) study. The LAMP assay has been validated according to the EPPO standard PM7/98. The LAMP assay was tested with lettuce seeds, soil and plant material, and can be used successfully to amplify DNA from each of these matrices. In seed lots artificially inoculated with FOL, the detection limit of the LAMP test was 0.004% infected seed.

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“…ortaya çıktığı görülmektedir. Avrupa'da marulda Fusarium solgunluğu (Gilardi, 2017) ve İspanya ve Kanada'da biberde solgunluk hastalığının yanı sıra kök ve kök boğazı hastalıklarının yaygınlaştığı rapor edilmiştir (Pérez-Hernández vd., 2014;Cerkauskas, 2017). Son yıllarda domateste solgunluk etmeninin yanı sıra kök ve kök boğazı çürüklük hastalığına neden olan F. oxysporum f. sp.…”

Section: Patojenisite çAlışmalarıunclassified

Identification and Virulence of Fusarium Wilt and Fusarium Crown & Root Rot Disease Agents from Tomato Greenhouses in Mediterranean Climate

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et al. 2023

Uluslararası Tarım Ve Yaban Hayatı Bilimleri Dergisi

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Akdeniz ikliminde Fusarium solgunluğu (Fusarium oxysporum f. sp. lycopersici, Fol) ve Fusarium kök ve kök boğazı çürüklüğü (Fusarium oxysporum f. sp. radicis-lycopersici, Forl) hastalıklarına neden olan fungal etmenler survey çalışmaları ile belirlenmiştir. İzolasyonlar sonucu elde edilen her iki etmene ait izolatların tür/ırk spesifik primerlerle (FOF1-FOR1, sp13, sp23 ve sprl) tanısı yapılmış ve virülenslik grupları oluşturulmuştur. Antalya ilinden izole edilen 42 adet Fol izolatının 20’si ırk 1 (R1), 7’si ırk 2 (R2) ve 15’i ırk 3 (R3) olarak saptanmıştır. Mersin ilinden izole edilen 3 adet Fol izolatı ırk 3 (R3) olarak belirlenmiştir. PCR çalışmalarında sprl primer seti, 58 adet Forl izolatı için 947-bp büyüklüğünde tek amplikon vermiştir. Domates bitkisinden izole edilen toplam 119 adet F. oxysporum izolatının 45’i Fol ve 58’i Forl olarak saptanmıştır. Virülenslikleri açısından, Fol izolatlarının %11’i yüksek virülent (YV), %58’i orta virülent (OV) ve %31’i düşük virülent (DV), Forl izolatlarının ise %28’i yüksek virülent (YV) ve %72’si orta virülent (OV) olarak saptanmıştır.

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Presence of Fusarium Wilt, Incited by Fusarium oxysporum f.sp. lactucae, on Lettuce in France

Cited by 15 publications

References 3 publications

Fusarium isolates from Belgium causing wilt in lettuce show genetic and pathogenic diversity

Fusarium isolates from Belgium causing wilt in lettuce show genetic and pathogenic diversity

Rapid detection of Fusarium oxysporum f. sp. lactucae on soil, lettuce seeds and plants using loop‐mediated isothermal amplification

Identification and Virulence of Fusarium Wilt and Fusarium Crown & Root Rot Disease Agents from Tomato Greenhouses in Mediterranean Climate

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