Presence of human papillomavirus DNA in breast cancer: a Spanish case-control study

Delgado-García, Silvia; Martínez‐Escoriza, Juan Carlos; Alba, A; Martín-Bayón, Tina; Ballester-Galiana, Hortensia; Peiró, Gloria; Caballero, Pablo; Ponce, José

doi:10.1186/s12885-017-3308-3

Cited by 48 publications

(52 citation statements)

References 66 publications

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“…HPV DNA sequences were first reported in BC samples in 1992 13 . Subsequently, several studies have documented the presence of HPV DNA in BC tissues 12 , 14 , 15 . Despite the emergence of HPV in BC as a major public health issue worldwide, only one previous study has been reported in Brazil; it was conducted in Porto Alegre and found HPV 16 and 18 in 24.5% of the cases 16 .…”

Section: Introductionmentioning

confidence: 99%

Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence

Cavalcante

Pinheiro

Almeida

et al. 2018

Clinics

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OBJECTIVE:The aim of this study is to investigate the presence of human papillomavirus DNA and genotypes in breast cancer and normal breast tissue samples obtained from women from the northeast region of Brazil.METHOD:One hundred three breast cancer samples and 95 normal breast samples, as the non-malignant controls, were studied. DNA extraction was verified by human beta-globin gene amplification, and polymerase chain reaction was conducted based on HPV L1-specific consensus primers MY09/MY11 and GP5+/GP6+, followed by nested multiplex polymerase chain reaction with type-specific primers for the E6/E7 consensus region.RESULTS:Human papillomavirus DNA was detected in 51 (49.5%) breast carcinoma samples and 15 (15.8%) normal breast samples (p<0.0001). Human papillomavirus genotypes 6 and 11 were identified in 15.2% of all samples.CONCLUSIONS:The high frequency of human papillomavirus infection in breast cancer samples indicates a potential role of this virus in breast carcinogenesis in the studied participants.

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Section: Introductionmentioning

confidence: 99%

Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence

Cavalcante

Pinheiro

Almeida

et al. 2018

Clinics

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“…There is substantial evidence which indicates a potential role for high risk HPVs in breast cancer. This evidence includes (i) meta-analyses of 22 studies conducted in a wide range of countries indicate that infection with high risk HPVs is associated with an increased risk of breast cancer with an overall odds ratio of 5.4, there are marked differences between countries in the prevalence of high risk HPVs in breast cancer [ 29 ], (ii) recent studies in the United Kingdom and Spain also indicate an increased risk of breast cancer associated with HPV infection [ 30 , 31 ], the risk differs according to HPV type, with 5.7 fold higher risk for HPV 16, 3.6 fold increase for HPV 33 and 3.0 fold increase for HPV 18 [ 29 ], (iii) HPVs immortalize and transform human mammary epithelial cells [ 32 ], (iii) HPV E6 and E7 oncoproteins have been identified in breast cancers, however, there is a low level of transcription of these oncoproteins which suggests that any influences of HPVs in breast cancer are likely to be indirect [ 4 , 33 ], (iv) high risk HPVs have been identified in the SK-BR-3 breast cancer cell line [ 34 ], (v) HPV type 16 E6 and E7 oncoproteins convert non-invasive and non-metastatic breast cancer cells to invasive and metastatic phenotypes [ 35 ], (vi) HPV-positive breast cancer is more common in young as compared to older women, an observation which is compatible with sexual transmission of HPV among sexually active younger women [ 36 ], (vi) HPV positive koilocytes have been identified in breast cancers [ 37 ], (vii) the prevalence of HPV positive breast cancer is higher in women with prior HPV associated cervical cancer [ 38 ], (viii) high risk HPV gene sequences are present in benign breast tissues prior to the development 3 to 10 years later of same HPV type positive breast cancers [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Multiple oncogenic viruses are present in human breast tissues before development of virus associated breast cancer

Lawson

Glenn

2017

Infect Agents Cancer

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BackgroundMultiple oncogenic viruses including, mouse mammary tumor virus, bovine leukemia virus, human papilloma virus, and Epstein Barr virus, have been identified as separate infectious pathogens in human breast cancer. Here we demonstrate that these four viruses may be present in normal and benign breast tissues 1 to 11 years before the development of same virus breast cancer in the same patients.MethodsWe combined the data we developed during investigations of the individual four oncogenic viruses and breast cancer. Patients who had benign breast biopsies 1–11 years prior to developing breast cancer were identified by pathology reports from a large Australian pathology service (Douglas Hanly Moir Pathology). Archival formalin fixed specimens from these patients were collected. The same archival specimens were used for (i) investigations of mouse mammary tumour virus (also known as human mammary tumour virus) conducted at the Icahn School of Medicine at Mount Sinai, New York and at the University of Pisa, Italy, (ii) bovine leukemia virus conducted at the University of California at Berkeley,(iii) human papilloma virus and Epstein Barr virus conducted at the University of New South Wales, Sydney, Australia.Seventeen normal breast tissues from cosmetic breast surgery conducted on Australian patients were used as controls. These patients were younger than those with benign and later breast cancer.ResultsStandard and in situ polymerase chain reaction (PCR) methods were used to identify the four viruses. The detailed methods are outlined in the separate publications.: mouse mammary tumor virus, human papilloma virus and Epstein Barr virus (Infect Agent Cancer 12:1, 2017, PLoS One 12:e0179367, 2017, Front Oncol 5:277, 2015, PLoS One 7:e48788, 2012).Epstein Barr virus and human papilloma virus were identified in the same breast cancer cells by in situ PCR.Mouse mammary tumour virus was identified in 6 (24%) of 25 benign breast specimens and in 9 (36%) of 25 breast cancer specimens which subsequently developed in the same patients.Bovine leukemia virus was identified in 18 (78%) of 23 benign breast specimens and in 20 (91%) of 22 subsequent breast cancers in the same patients.High risk human papilloma viruses were identified in 13 (72%) of 17 benign breast specimens and in 13 (76%) of 17 subsequent breast cancers in the same patients.Epstein Barr virus was not identified in any benign breast specimens but was identified in 3 (25%) of 12 subsequent breast cancers in the same patients.Mouse mammary tumour virus 3 (18%), bovine leukemia virus 6 (35%), high risk human papilloma virus 3 (18%) and Epstein Barr virus 5 (29%) were identified in 17 normal control breast specimens.ConclusionsThese findings add to the evidence that multiple oncogenic viruses have potential roles in human breast cancer. This is an important observation because evidence of prior infection before the development of disease is a key criterion when assessing causation.

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“…Breast cancer is the most commonly diagnosed malignancy in women. It is estimated that 1.7 million new cases were diagnosed in 2012, representing 11.9% of all cancers diagnosed worldwide in both genders, and 25% of those diagnosed in women [1]. Analyses using Globocan 2012 data have reported that one out of every 25 women in Turkey will develop breast cancer in some period of their lives [2].…”

Section: Introductionmentioning

confidence: 99%

“…The immortalization of HPV 16 and 18 genomes in a group of human breast cells after the infection may cause breast cancer associated with HPV [4]. The current published data on HPV and breast cancer are very contradictory, since the reported prevalence of HPV ranges from 0% to 86.21% in breast cancer tissue samples [1].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Relationship between Breast Cancers ‎and HPVs

Saeed¹,

Zer²,

Bozdağ³

2018

ujcm

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High-risk human papillomaviruses (HR-HPVs) have been implicated in the aetiology of a variety of human cancers, including cervical, colorectal, and head and neck (HN); also, (HPVs) may be important risk factors for breast carcinogenesis and metastasis. Previous studies that evaluated the prevalence of HPVs in breast cancer have generated considerable controversy. Based on this hypothesis, we aimed to investigate the prevalence of HPV infection in invasive breast cancer tissues. Our study included 80 paraffin blocks taken from breast cancerous tissue specimens of Turkish female patients and 13 controls (cervical cancerous tissue specimens) in Research and Practice Hospital-Sahinbey-Gaziantep University, using PCR (HPV Genotypes 14 Real-TM Quant / Italy) to detect the HR-HPV DNA. In our results: HR-HPV DNA was evidenced in (10%) of the breast cancer and in (92.3 %) of the controls (p < 0.05). HPV (45-56-68) were the most prevalent genotypes in breast cancer cases. Also HPV co-infection was high (75%) in positive breast cancerous samples. Our data propose Human papilloma viruses may play a role as a possible causal relationship, or mediation or even as a cofactor in the development of some types of breast cancer, this still needs to be confirmed by prospective studies.

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Presence of human papillomavirus DNA in breast cancer: a Spanish case-control study

Cited by 48 publications

References 66 publications

Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence

Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence

Multiple oncogenic viruses are present in human breast tissues before development of virus associated breast cancer

Investigation of the Relationship between Breast Cancers ‎and HPVs

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