Presence of Recombinant Strain of Cucurbit Aphid Borne Yellows Virus in Iran

Abstract

(CABYV) is among the most important yellowing viruses on cucurbits in Iran. The presence of CABYV has been previously reported from the major cucurbit growing areas in Iran, however, there are few studies concerning the detection of the different strains of this virus in the grower's fields, and especially, there is no report of the weed host plants near crop fields. This study was done in order to detect the new strains of the CABYV polerovirus in cucurbits and the weed plants in the Lorestan province, Iran, as an introductory investigation for initiating a program of the breeding for resistance. During a survey carried out in 2013-2014 in Lorestan province; Iran, 189 cucurbit and 261 weed samples were investigated for the presence of CABYV using RT-PCR. In addition, the phylogeny and nucleotide similarities were discussed on the basis of the partial nucleotide sequence of RNA dependent RNA polymerase (RdRP) gene. The RT-PCR carried out on leaf samples revealed the infection with the CABYV in 43 cucumber and 12 weed samples. RT-PCR using strain specific primers detected the presence of the both common (C) and recombinant (R) strains of CABYV in the tested samples. On the basis of the phylogenetic analyses, the CABYV-C isolates from Iran were clustered into two distinct sub-populations (CI and CII), such that all the weed samples with two sequenced cucumber isolates were clustered in the CI sub-population. Meanwhile, a distinct sub-population of the isolates was clustered in the CABYV-R group showed a shared sequence identity of 97% to a Taiwanese isolate (JQ700306). This study has indicated the incidence of CABYV-R in the Southwest Asia; Iran for the first time. We were also able to show CABYV occurrence in and from this area of the world. Identification of cucurbit infecting viruses and studying their distribution and potential reservoir hosts are important for developing successful control programs for virus disease management.

High-Throughput Sequencing Facilitates Discovery of New Plant Viruses in Poland

“…Moreover, plant viruses can emerge in crops from reservoir wild plant hosts in which they are often asymptomatic. Spread from the reservoirs into a new environment with the establishment of productive infections and effective between-host transmission mechanism are steps that require emergence to occur [ 10 ]. In addition, many wild plants appear to have multiple infections, including both acute and persistent viruses [ 11 ].…”

Characterization of Cucurbit Aphid-Borne Yellows Virus (CABYV) from Passion Fruit in Brazil: Evidence of a Complex of Species within CABYV Isolates

“…CABMV can infect plants of the families Passifloraceae, Fabaceae, Cucurbitaceae, Solanaceae, Chenopodiaceae, Amaranthaceae, and Poaceae [ 50 , 51 , 52 , 53 ]. CABYV has a wide range of Cucurbitacea plants as hosts and can infect several other plant families, such as Brassicaceae, Asteraceae, Malvaceae, Fabaceae, Amaranthaceae, Chenopodiaceae, Papaveraceae, Lamiaceae, Portulacaceae, Solanaceae, and Passifloraceae [ 16 , 17 , 20 , 27 , 32 , 54 , 55 , 56 , 57 , 58 ]. However, CABYV infection in Macroptilium spp., Stylosanthes spp., Sida spp., and Bignonia spp., as well as CABMV infection in Sida spp.…”

Metatranscriptome analysis of symptomatic bitter apple plants revealed mixed viral infections with a putative novel polerovirus

“…Building upon a previous research which identified WMV from bitter apple plants in the Jiroft region, our study aimed to explore a wider range of potential viruses in bitter apple plants showing viral symptoms in the Jiroft region. In addition to WMV, other studies in Iran have detected the presence of Papaya ring spot virus-type W and cucurbit aphid-borne yellows virus in this plant [ 4 – 5 ]. These findings indicate that this plant species can act as a viral reservoir affecting agricultural crops [ 4 – 8 ].…”