Preservation of proteinaceous material during the degradation of the green alga Botryococcus braunii : A solid-state 2D 15 N 13 C NMR spectroscopy study

Zang, Xu; Nguyen, Reno T.; Harvey, H. Rodger; Knicker, Heike; Hatcher, Patrick G.

doi:10.1016/s0016-7037(01)00679-2

Cited by 60 publications

(37 citation statements)

References 32 publications

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“…Although there is little evidence from the 15 N-NMR spectra that glycosylated protein accounts for a large fraction of HMW DON (McCarthy et al 1997), the above results still provide insights into interactions of bacteria with "modified" proteins such as glycoprotein (enzymatically modified protein), which has been reported to be abundant in dissolved protein in seawater (Yamada and Tanoue, 2003). Recent studies have suggested fundamental roles of noncovalent (hydrophobic interactions and encapsulation: Harvey 2001, 2003;Zang et al 2001) and covalent associations (Hsu and Hatcher 2005) of proteins with other organic matter in the long-term preservation of proteins.…”

Section: Polymeric Dom-protein As a Modelmentioning

confidence: 97%

Organic Matter–Bacteria Interactions in Seawater

Nagata

2008

Microbial Ecology of the Oceans

119

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Section: Polymeric Dom-protein As a Modelmentioning

confidence: 97%

Organic Matter–Bacteria Interactions in Seawater

Nagata

2008

Microbial Ecology of the Oceans

119

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“…The porous structure of humic acid facilitates inward diffusion of the small substrate molecule and outward diffusion of the product molecule, but at the same time, the movement of larger molecules is restricted. Hydrophobic interactions are mainly responsible for enzyme humic acid interaction (Tan et al, 2009;Zang et al, 2000Zang et al, , 2001. It is observed that enzyme activity of the organic matter bound enzyme is highly specific for the particular enzyme.…”

Section: Enzyme Organic Matter Interactionsmentioning

confidence: 99%

How enzymes are adsorbed on soil solid phase and factors limiting its activity: A Review

Datta¹,

Anand²,

Moulick³

et al. 2017

International Agrophysics

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A b s t r a c t. A majority of biochemical reactions are often catalysed by different types of enzymes. Adsorption of the enzyme is an imperative phenomenon, which protects it from physical or chemical degradation resulting in enzyme reserve in soil. This article summarizes some of the key results from previous studies and provides information about how enzymes are adsorbed on the surface of the soil solid phase and how different factors affect enzymatic activity in soil. Many studies have been done separately on the soil enzymatic activity and adsorption of enzymes on solid surfaces. However, only a few studies discuss enzyme adsorption on soil perspective; hence, we attempted to facilitate the process of enzyme adsorption specifically on soil surfaces. This review is remarkably unmatched, as we have thoroughly reviewed the relevant publications related to protein adsorption and enzymatic activity. Also, the article focuses on two important aspects, adsorption of enzymes and factors limiting the activity of adsorbed enzyme, together in one paper. The first part of this review comprehensively lays emphasis on different interactions between enzymes and the soil solid phase and the kinetics of enzyme adsorption. In the second part, we encircle various factors affecting the enzymatic activity of the adsorbed enzyme in soil.

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“…In follow-up studies, Harvey and coworkers [91][92][93] provided further support for the encapsulation hypothesis. Zang et al [91] conducted dual-labeling experiments using 13 C and 15 N to follow the degradation of Botryococcus braunii, a prolific producer of biopolymeric algaenan.…”

Section: Matrix Effectsmentioning

confidence: 93%

“…Zang et al [91] conducted dual-labeling experiments using 13 C and 15 N to follow the degradation of Botryococcus braunii, a prolific producer of biopolymeric algaenan. They found that biologically labile proteins and carbohydrates were preferentially lost during the time course of the experiment, but proteinaceous material remained the major form of organic N even after 200 days.…”

Section: Matrix Effectsmentioning

confidence: 99%