Pretreatment optimization of the biomass of Microcystis aeruginosa for efficient bioethanol production

Abstract

Microalgae are considered to be the future promising sources of biofuels and bio products. The algal carbohydrates can be fermented to bioethanol after pretreatment process. Efficient pretreatment of the biomass is one of the major requirements for commercialization of the algal based biofuels. In present study the microalga, M. aeruginsa was used for pretreatment optimization and bioethanol production. Treatment of algal biomass with CaO before acid and/or enzymatic hydrolysis enhanced the degradation of algal cells. Monomeric sugars yield was increased more than twice when biomass was pretreated with CaO. Similarly, an increase was noted in the amount of fermentable sugars when biomass was subjected to invertase saccharification after acid or lysozyme pretreatment. Highest yield of fermentable sugars (16 mM/ml) in the centrifuged algal juice was obtained. 4 Different microorganisms’ species were used individually and in combination for converting centrifuged algal juice to bioethanol. Comparatively higher yield of bioethanol (60 mM/ml) was obtained when the fermenter microorganisms were used in combination. The results demonstrated that M. arginase biomass can be efficiently pretreated to get higher yield of fermentable sugars for enhanced yield of bioethanol production.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-016-0320-y) contains supplementary material, which is available to authorized users.

An effective acid pretreatment of agricultural biomass residues for the production of second-generation bioethanol

“…The physical and chemical pretreatments performed on beet and date materials were required to reduce recalcitrance biomass for hydrolyzing complex sugars (mainly sucrose) into simple intermediate products and increase the reactive surface area [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. A comparison of the yield and concentration of ethanol of all fermentation processes is presented in Table 3.…”

“…Agricultural residues are rich in organic matter but are unfortunately underutilized and often end up being polluters of the environment. These by-products can be valorized in order to produce bioethanol which can be used as alone or can be blended with gasoline as transportation fuels [5][6][7]. Bioethanol can be produced through fermentation of any raw materials as long as it contains sugar [8,9].…”

“…Thus, an efficient pretreatment should increase the content of fermentable sugars (glucose, fructose, galactose, etc.) and will results in a good yield of fermentative bioethanol [6,10,11]. Palm date is very rich in sucrose, fructose and glucose.…”

Optimization of Fermentation Conditions for Ethanol Production from Renewable Biomass Using Response Surface Methodology

“…Pre-treatment with CaO before enzyme hydrolysis increases reducing sugar content for fermentation. A combination of microorganisms was used for fermentation [16]. The paper discusses the possibility of simultaneous utilization of glucose and xylose by Saccharomyces cerevisiae for production of ethanol on large scale, as xylose is an important component of most agricultural residues.…”

Screening of fungal strains with potentiality to hydrolyze microalgal biomass by Fourier Transform Infrared Spectroscopy (FTIR)

“…(John, Anisha, Nampoothiri, & Pandey, 2011). These microorganisms have a high photosynthetic rate and considerable biodiversity and variability in biochemical composition, accumulating considerable amounts of lipids and carbohydrates (Brennan & Owende, 2010;Khan, Lee, Shin, & Kim, 2017). Besides that, microalgae present efficient assimilation of cellulose and have high concentrations of starch.…”