Pretreatment with potassium channel blockers of 4-aminopyridine and tetraethylammonium attenuates behavioural symptoms of Parkinsonism induced by intrastriatal injection of 6-hydroxydopamine; the role of lipid peroxidation

Haghdoost-Yazdi, Hashem; Piri, Hossein; Faraji, Ayda; Fraidouni, Negin; Dargahi, Tahereh; Mahmudi, Marzieh; Heidari, Mahmud Alipour

doi:10.1080/01616412.2015.1114290

Cited by 8 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, genistein significantly raised the blood pressure in animals pre-treated with methylene blue (a monoamine oxidase and cGMP inhibitor), Tetraethylammonium (a potassium channel blocker) and Nifedipine (a calcium channel inhibitor). Although Tetraethylammonium, methylene blue and nifedipine are known blood pressure lowering drugs that produce a reduction in blood pressure via blockade of K + , cGMP induced pressure natriuresis and calcium channels respectively [11,13,14], intravenous genistein administration attenuated these blood pressure lowering effects of the drugs. Richardson et al, (2016) [23] reported that methylene blue had no effect on relaxation induced by genistein.…”

Section: Discussionmentioning

confidence: 99%

“…This procedure was repeated for each of the drugs in the different groups. The drugs administered include Indomethacin, a non-steroidal anti-inflammatory and a cyclooxygenase (COX-2) enzyme inhibitor (5 mg/kg subcutaneously), methylene blue, an inhibitor of nitric oxide synthase and guanylate cyclase (7.5 mg/kg, by infusion), nifedipine,a calcium channel blocker (0.75 mg/kg intravenously), and tetraethylammonium a voltage-gated K + channels blocker (60 mg/kg intravenously) [11][12][13][14]. Genistein was administered in graded doses of 0.008, 0.016, 0.032, 0.064, 0.128, and 0.256 mg/kg body weight intravenously [10], after the administration of the drugs to determine the effect and possible mechanism of actions of genistein on the blood pressure.…”

Section: Preparation and Administration Of Genistein And Different Drugsmentioning

confidence: 99%

See 1 more Smart Citation

Genistein increases blood pressure in pre-treated normotensive rats

Awobajo,

Ogbu,

Adejare

et al. 2023

J Pre Clin Clin Res.

View full text Add to dashboard Cite

Introduction and Objective.High blood pressure is an important risk factor for heart-related disease. Combining dietary adjustments with standard drugs in the therapy of heart diseases have gained attention. Genistein, a soy isoflavone, has many biological effects but its role in blood pressure is inconclusive. The aim of the study was to investigate the effect of genistein on blood pressure in normotensive rats pre-treated with standard drugs. Materials and method. Twenty healthy adult male Sprague Dawley rats (body weight 160-180g) were administered with genistein, and SBP, DBP, PP, MABP and HR were recorded. Subsequently, the rats (5 each) were treated with Indomethacin, a non-steroidal, anti-inflammatory and a cyclooxygenase (COX-2) enzyme inhibitor (5 mg/kg subcutaneously), methylene blue,an inhibitor of nitric oxide synthase and guanylate cyclase (7.5 mg/kg, by infusion), nifedipine,a calcium channel blocker (0.75 mg/kg intravenously), and tetraethylammonium, a voltage-gated K+ channels blocker (60 mg/kg intravenously). Genistein was administered intravenously in graded doses after the administration of these blockers. SPB, DBP, PP and MABP were significantly increased following genistein administration only, as well as in the methylene blue and tetraethylammonium groups each. Results. Higher doses of genistein significantly decreased SBP, DBP and PP after indomethacin treatment. Falling DBP and MABP were reversed in the nifedipine group. Conclusions. Results suggests that genistein elevates blood pressure in experimental normotensive male rats. This has implications for alternative therapy in the management of hypertension, and implications for blood pressure in cardiovascular related diseases

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Preparation and Administration Of Genistein And Different Drugsmentioning

confidence: 99%

Genistein increases blood pressure in pre-treated normotensive rats

Awobajo,

Ogbu,

Adejare

et al. 2023

J Pre Clin Clin Res.

View full text Add to dashboard Cite

show abstract

“…1,1,3,3-tetra methoxy propane was used as standard. It should also be noted that MDA reacts with TBA to produce a pink-colored solution that has a maximum absorbance at 532 nm [22].…”

Section: Methodsmentioning

confidence: 99%

The Effects of Oral Silver Nanoparticles and Propolis on the Level of Lipid Peroxidation in Male Wistar Rats

Piri

Mohammadi

Aali

et al. 2021

The Journal of Qazvin University of Medical Sciences

Self Cite

View full text Add to dashboard Cite

Objective: Oxidative stress is a leading factor in the development of silver nanoparticle-induced toxicity. This study evaluated the effects of oral prescription of different doses of silver nanoparticles and propolis on lipid peroxidation in male Wistar rats. Materials and Methods: The male wistar rats were randomly divided into four groups. Control group and three different treatment groups with oral prescription of 30 ppm, 60 ppm, and 60 ppm of silver nanoparticles with 200 mg/kg of propolis. The Serum level of malondialdehyde (MDA) was measured by TBARS assay using Thiobarbituric acid (TBA). Results: The Serum level of MDA as a marker of lipid peroxidation in the control group was 1.92 mM/ml, and in other groups that received silver nanoparticles (30 ppm,60 ppm, 60 ppm with 200 mg/kg propolis) were respectively 2.82, 3.83 and 2.62 mM/ml. MDA level also increased at the doses of 30 ppm and 60 ppm compared to the control group and decreased when propolis was prescribed with silver nanoparticles in the third group; however, its value did not reach the level of the control group. Minimal levels of serum lipid peroxidation were observed when silver and propolis nanoparticles were administered to male rats. Conclusion: The mixture of silver nanoparticles and propolis reduces the toxic effects of silver nanoparticles; not only it preserves but also increases the efficiency of this compound in medical applications.

show abstract

“…Total Anti-oxidant Capacity (TAC) in seminal plasma was determined according to Ferric reducing Anti-oxidant Power (FRAP) assay using small modifications [19]. Briefly, ferric tripyridyltriazine (Fe 3+ -TPTZ) complex was reduced to ferrous form (Fe 2+ -TPTZ) by the anti-oxidants in the sample at acidic pH [20,21].…”

Section: Anti-oxidant Activitymentioning

confidence: 99%

Anethum graveolens L‎. Alleviates Sperm ‎Damage by Limiting Oxidative Stress ‎and Insulin ‎Resistance ‎in ‎Diabetic Rats ‎

Abbasi-Oshaghi¹,

Khodadadi²,

Mirzaei³

et al. 2020

TOMCJ

View full text Add to dashboard Cite

Background: It has been reported that diabetes is associated with sperm ‎damage and infertility. Objective: The purpose of this experiment was to survey the effect of Anethum graveolens L. (Dill) powder on sperm profiles, oxidative stress, insulin resistance, and histological changes in male diabetic rats. Methods: Male rats were randomly divided into 6 groups (n=7); group 1: normal rats, 2: normal rats + 100mg/kg Dill, 3: normal rats + 300mg/kg Dill, 4: diabetic rats, 5: diabetic rats + 100mg/kg Dill, and 6: diabetic rats + 300mg/kg Dill. After 2 months of treatments, the sperm profile, anti-oxidant activity, superoxide dismutase (SOD) activity, and malondialdehyde were measured. The histopathology of testis was evaluated. Hormonal changes and tumor necrosis factor-α (TNF-α) levels were measured by ELISA. Results: Total anti-oxidant and SOD activity in diabetic rats significantly decreased, while MDA concentration was significantly increased in the testis and pancreas of diabetic rats compared with control. However, the use of Dill significantly normalized these profiles. The treatment of diabetic rats with Dill changed the sperm parameters. The levels of testosterone, FSH, and LH in diabetic rats were significantly reduced, but the treatment with Dill did not alter the level of these hormones. Dill also significantly normalized testis morphological changes, insulin resistance, and inflammation. Conclusion: The use of Dill normalized oxidative stress, inflammation, and insulin resistance in diabetic rats that correlated with sperm profile and testis histological changes. The treatment of diabetic rat models with Dill did not show harmful effects on sperm profiles.

show abstract

Pretreatment with potassium channel blockers of 4-aminopyridine and tetraethylammonium attenuates behavioural symptoms of Parkinsonism induced by intrastriatal injection of 6-hydroxydopamine; the role of lipid peroxidation

Cited by 8 publications

References 35 publications

Genistein increases blood pressure in pre-treated normotensive rats

Genistein increases blood pressure in pre-treated normotensive rats

The Effects of Oral Silver Nanoparticles and Propolis on the Level of Lipid Peroxidation in Male Wistar Rats

Anethum graveolens L‎. Alleviates Sperm ‎Damage by Limiting Oxidative Stress ‎and Insulin ‎Resistance ‎in ‎Diabetic Rats ‎

Contact Info

Product

Resources

About