Prevalence and Characteristics of Aeromonas Species Isolated from Processed Channel Catfish

Abstract

From August 1994 to May 1995, 238 channel catfish fillets collected from three processing plants in the Mississippi Delta at four time periods were tested for the presence of Aeromonas species. Identification of Aeromonas spp. was accomplished using an automated Vitek bioassay system with gram-negative and nonfermenter cards. Approximately 36.1% were positive for A. hydrophila, 35.7% for A. sobria, and 10.9% for A. caviae. All three Aeromonas spp. were found in all three processing plants, and the incidence of A. hydrophila contamination appeared to be higher in summer than other seasons. Eighty-six percent of the Aeromonas isolates were hemolytic on 5% sheep blood agar plates. Most isolates were susceptible to chloramphenicol, neomycin, streptomycin, and trimethoprim-sulfamethoxazole and resistant to ampicillin and bacitracin. Results suggest that Aeromonas spp. are prevalent in processed channel catfish, and most isolates are hemolytic and resistant to ampicillin and bacitracin.

Determination of beta-haemolytic activity and minimum inhibitory concentrations of antimicrobial drugs against Aeromonas hydrophila strains isolated from fish products

“…All strains in our study were sensitive to gentamicin at concentrations of ≤4 µg/mL. Other researchers (Wang & Silva, 1999;Vila et al, 2002;Hatha et al, 2005;Ottaviani et al, 2006;Awan et al, 2009) have also confirmed that A. hydrophila was sensitive to gentamicin. Unlike us, Thayumanavan et al (2003) showed a resistance to gentamicin (3.6%).…”

“…could grow and release enterotoxin and haemolysin at fridge temperature conditions (Sharma & Kumar, 2011). A number of researchers have confirmed the presence of β-haemolytic aeromonads in fish products (Tsai & Chen, 1996;Wang & Silva, 1999;Radu et al, 2003;Thayumanavan et al, 2003;Yuсel et al, 2005;Erdem et al, 2010). A special attention is paid on A. hydrophila as a causative agent of foodborne gastroenterites, and fish products as the commonest vector of this microbial pathogen (Thayumanavan et al, 2003;Hatha et al, 2005;Paniagua et al, 2006).…”

Isolation and identification of motile Aeromonas spp. in turkey meat

“…Pathogens are mostly isolated from; rivers, lakes, sewers, clorinated drinking water (1,5,12,17,24,25,31), retail fresh vegetables (33), sea foods (1, 2, 17), red and minced meat (10,22,26,29,37), raw and pasteurized milk (21,35), unpasteurized cheese (34). It is also widespread in fresh water fishes (14,28,40). There are only limited studies on determination of motile Aeromonas spp.…”

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“…The incidence of A. hydrophila in fish samples investigated in this study was lower in comparison to those detected in U.S. by Wang & Silva (1999) Nagar et al (2011) stated the presence of A. hydrophila in 3.85% of 52 freshwater fish samples. In the current study, the incidence of A. hydrophila in fish was 3.75%.…”