Prevalence and characterization of extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase producing Enterobacteriaceae in fresh pork meat at processing level in Germany

Schill, Franziska; Abdulmawjood, Amir; Klein, Günter; Reich, Felix

doi:10.1016/j.ijfoodmicro.2017.06.010

Cited by 45 publications

(33 citation statements)

References 38 publications

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“…ESBL-producing Enterobacteriaceae were detected across all six process steps with an overall prevalence of 6.1 %. This was lower compared to previous research performed in (Pacholewicz, Liakopoulos, et al, 2015a;Reich, Atanassova, & Klein, 2013;Schill, Abdulmawjood, Klein, & Reich, 2017), which is probably owed to the quantitative analysis in the present study compared to more sensitive qualitative testing. This is also the most probable reason why none of the samples from the smallest group, organic broilers, were ESBL positive.…”

Section: ! Discussioncontrasting

confidence: 89%

Quantitative microbiological slaughter process analysis in a large-scale Swiss poultry abattoir

et al. 2019

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We performed a quantitative microbiological slaughter process analysis in a large-scale Swiss poultry abattoir. At each of six selected process steps (plucking, cloaca excision, evisceration, washing, water spray chiller and dry air chiller), 103 carcasses from 50 flocks were sampled and quantitatively tested for indicator bacteria (total viable counts, Escherichia (E.) coli/Enterobacteriaceae counts) as well as for Campylobacter spp. and extended-spectrum beta-lactamase-producing Enterobacteriaceae. Samples consisted of a pooled back, breast and leg skin sample as well as swab samples from the thoracic and abdominal cavities. On skin samples, the presence of E. coli and Enterobacteriaceae increased between plucking and evisceration (on average by 1.2 log CFU/g), while washing decreased the microbial load (on average by 0.8 log CFU/g). Subsequent chilling slightly reduced counts on carcasses skin and markedly reduced the counts in the abdominal cavity. ESBL-producing E. coli was present above the limit of detection (LOD) with a prevalence of 6.1%, the majority of which belonged to phylogenetic group A. The bla CTXM-1 gene was found in all ESBL producing strains. The prevalence of Campylobacter above the LOD was 16.7%, and none of the final, chilled carcasses had counts that exceeded 1000 CFU/g. Multilocus sequence typing of 120 Campylobacter jejuni strains yielded 15 different sequence types (ST) among which ST 21 was predominant with 31.7% and four newly defined ST.

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Section: ! Discussioncontrasting

confidence: 89%

Quantitative microbiological slaughter process analysis in a large-scale Swiss poultry abattoir

et al. 2019

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“…K lebsiella pneumoniae presenta un incremento considerable como agente causal de infecciones intrahospitalarias de difícil tratamiento, con afectación muy variada: tracto urinario, pulmones, tejidos blandos, área quirúrgica y sepsis; también se ha reportado un aumento en la prevalencia de K. pneumoniae resistente a antimicrobianos, entre ellos a cefalosporinas de tercera generación, provocando estancias hospitalarias prolongadas y una tasa de mortalidad de 27,3% [1][2][3] .…”

Section: Introductionunclassified

“…La producción de enzimas β-lactamasas de espectro extendido (BLEE), que son capaces de inactivar a las cefalosporinas de espectro extendido como ceftazidima (CAZ), cefotaxima, ceftriaxona y aztreonam, han contribuido significativamente al aumento de la resistencia hacia los antibacterianos β-lactámicos. La diseminación de los genes que codifican para estas enzimas representa un problema de magnitud global, con altas tasas de producción de BLEE en algunas especies de enterobacterias como K. pneumoniae y Escherichia coli 1,4 .…”

Section: Introductionunclassified

Caracterización molecular y detección de genes blaCTX-M grupos 1 y 9 en Klebsiella pneumoniae resistentes a ceftazidima, en un hospital de San José de Cúcuta, Colombia

Galvis

2019

Rev. chil. infectol.

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“…Studies have shown a high frequency of ESBLproducing bacterial isolates in farm animals and products of animal origin (KOGA et al, 2015;FISCHER et al, 2013;SCHILL et al, 2017). In our study, high levels of resistance were found against the β-lactam: AMO (100%); AMP (100%); monobactams: ATM (72.72%); first-generation cephalosporin: CFZ (100%); third-generation: CAZ (45.45%), CTX (72.72%), CTF (72.72%), CRO (72.72%) and fourth generation: CPM (72.72%).…”

Section: Discussionmentioning

confidence: 99%

Multidrug resistant and ESBL-producing Salmonella spp. isolated from poultry

Souza

Brito

Menck-Costa

et al. 2019

SCA

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Salmonella spp. is one of the main agents responsible for foodborne infection in humans, and products of poultry origin are the most common infection sources. Studies have shown the occurrence of antimicrobials resistant Salmonella spp. in animal products. The Extended Spectrum ?-Lactamase (ESBL) are enzymes that confer to bacteria the ability to hydrolyze cephalosporin with an oximino side chain and monobactams. This study aimed to investigate antimicrobial resistance profile, identify phenotypes and genotypes for multiple drug resistance (MDR) and that produce ESBL from isolates of Salmonella spp. in the broiler production chain. We used samples of Salmonella spp. (n=11) isolates from poultry, poultry products and poultry-source environment from the state of Maranhão - Brazil. The isolates of Salmonella spp. assessed showed genotypical and phenotypical characteristics of MDR. The results show that 72.72% (08/11) of the strains presented the phenotypic profile for ESBL production. The isolates showed positivity to at least 13.64% (03/22) of the genes studied and the highest frequencies were observed in genes sul1 (73%), dfrA12 (55%), blaCTX-M (55%), tetA, tetB and tetC, with 45%. In conclusion, the strains of Salmonella spp. isolates present genotypic and phenotypic characteristics for MDR and ESBL production, demonstrating the dissemination risk of these microorganisms through the food chain.

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Prevalence and characterization of extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase producing Enterobacteriaceae in fresh pork meat at processing level in Germany

Cited by 45 publications

References 38 publications

Quantitative microbiological slaughter process analysis in a large-scale Swiss poultry abattoir

Quantitative microbiological slaughter process analysis in a large-scale Swiss poultry abattoir

Caracterización molecular y detección de genes blaCTX-M grupos 1 y 9 en Klebsiella pneumoniae resistentes a ceftazidima, en un hospital de San José de Cúcuta, Colombia

Multidrug resistant and ESBL-producing Salmonella spp. isolated from poultry

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