Prevalence and characterization of Staphylococcus aureus isolates from subclinical bovine mastitis in southern Xinjiang, China

Ren, Qiang; Liao, Guanghua; Wu, Zihao; Lv, Junfan; Chen, Wei

doi:10.3168/jds.2019-17420

Cited by 79 publications

(72 citation statements)

References 46 publications

(88 reference statements)

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“…Bovine mastitis is a major disease of dairy cows, which has a great impact on the dairy industry and brings significant economic losses (Hata et al 2010;Li et al 2017;Wolf et al 2011;Zaatout et al 2020). S. aureus is the most frequently recovered pathogen from mastitis cases (Hata et al 2010;Ote et al 2011;Ren et al 2020), and responsible for subclinical and persistent intramammary infections. Multiple studies reported the diversity of genotype and phenotype of S. aureus strains obtained from bovine mastitis (Haveri et al 2005;Suleiman et al, 2018;Piccinini et al 2012;Ronco et al 2018;Veh et al 2015).…”

Section: Bovine Mastitis and Endometritismentioning

confidence: 99%

“…Further, 161 isolates possessed at least 2 SAg genes (Katsuda et al 2005;Hata et al 2006). S. aureus strains isolated from bovine mastitic milk were separated into 60 patterns and 16 lineages by pulse field gel electrophoresis, and the most common combinations of toxin genes were sec, seg, sei, sel, and tst; or seg and sei; or sec, seg, sei, sel, sen and tst (Hata et al 2010;Hoekstra et al 2020;Li et al 2017;Ren et al 2020). The predominant isolate possessed SAg toxin genes supporting the theory that SAg toxins are important for udder pathogenesis of bovine mastitis.…”

Section: Bovine Mastitis and Endometritismentioning

confidence: 99%

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Update on molecular diversity and multipathogenicity of staphylococcal superantigen toxins

Fang

et al. 2021

Animal Diseases

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Staphylococcal superantigen (SAg) toxins are the most notable virulence factors associated with Staphylococcus aureus, which is a pathogen associated with serious community and hospital acquired infections in humans and various diseases in animals. Recently, SAg toxins have become a superfamily with 29 types, including staphylococcal enterotoxins (SEs) with emetic activity, SE-like toxins (SEls) that do not induce emesis in primate models or have yet not been tested, and toxic shock syndrome toxin-1 (TSST-1). SEs and SEls can be subdivided into classical types (SEA to SEE) and novel types (SEG to SElY, SE01, SE02, SEl26 and SEl27). The genes of SAg toxins are located in diverse accessory genetic elements and share certain structural and biological properties. SAg toxins are heat-stable proteins that exhibit pyrogenicity, superantigenicity and capacity to induce lethal hypersensitivity to endotoxin in humans and animals. They have multiple pathogenicities that can interfere with normal immune function of host, increase the chances of survival and transmission of pathogenic bacteria in host, consequently contribute to the occurrence and development of various infections, persistent infections or food poisoning. This review focuses on the following aspects of SAg toxins: (1) superfamily members of classic and novelty discovered staphylococcal SAgs; (2) diversity of gene locations and molecular structural characteristics; (3) biological characteristics and activities; (4) multi-pathogenicity of SAgs in animal and human diseases, including bovine mastitis, swine sepsis, abscesses and skin edema in pig, arthritis and septicemia in poultry, and nosocomial infections and food-borne diseases in humans.

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Section: Bovine Mastitis and Endometritismentioning

confidence: 99%

Section: Bovine Mastitis and Endometritismentioning

confidence: 99%

Update on molecular diversity and multipathogenicity of staphylococcal superantigen toxins

Fang

et al. 2021

Animal Diseases

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“…Ribeiro et al (2009) in a study on pathogenic microorganisms in bovine milk, found the highest resistance rates of 53.5% when the strains ISSN 2166-0379 2020 were submitted to penicillin. Similarly, Ren et al (2020) investigated the prevalence and antimicrobial susceptibility of Staphylococcus aureus from subclinical bovine mastitis in dairy farms located in China. The authors found the resistance rates to penicillin, tetracycline, and chloramphenicol were 58.5, 18.5, and 1.5%, respectively.…”

Section: Journal Of Agricultural Studiesmentioning

confidence: 99%

Microbiological Evaluation of Milk Quality and Antibiogram in Dairy Cows Managed on Pasture

Ítavo¹,

Ítavo²,

Dias³

et al. 2020

JAS

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Mastitis is a disease in dairy cattle that damage the milk chain. It was aimed identify the major causative agents of bovine mastitis in small dairy farms by producing an antibiogram and analysis of milk quality. Methods: During the summer, 280 dairy cows were examined on a farm located in Camapuã, MS, Brazil. The farm had a concrete milking facility and used cleaning and disinfection of the udder before and after milking to control mastitis. For assess microbial resistance, two samples of milk per cow were collected biweekly between January and March. The antibiotics sulfazotrim (25 μg), penicillin (10 μg), streptomycin (10 μg), vancomycin (30 μg), chloramphenicol (30 μg), tetracycline (30 μg), amoxicillin (10 μg), and gentamycin (10 μg) were used. Results: A total of 17.14% of the animals were positive for the California Mastitis Test (CMT). The identification of the pathogens revealed that Staphylococcus aureus accounted for 41.65% of the isolated organisms and Escherichia coli for 37.5%. Our results showed only sulfazotrim and chloramphenicol had effective results for both Gram-negative and Gram-positive bacteria. The isolated strains presented high resistance to the other tested antibiotics. Conclusions: sulfazotrim (25 μg) and chloramphenicol (30 μg) can be used to disinfect the udder of dairy cows.

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“…Several characteristics contribute to the pathogenesis and spread of S. aureus, including virulence factors, host, and environment (ACOSTA et al, 2018). Its ability to adhere and form biofilms on biotic and abiotic surfaces significantly increases the persistence of this pathogen in equipment and facilities, providing a physiological advantage as an etiological agent of diseases caused by food consumption, especially through the consumption of milk and its derivatives (MERGHNI et al, 2015;WANG et al, 2018;REN et al, 2020) The formation of biofilms contributes to the resistance to antimicrobials (MELCHIOR et al, 2006;RAZA et al, 2013). Moreover, the excessive use of antimicrobials has been shown to favor the emergence and selection of resistant bacteria, altering the structure of bacterial communities and inducing a rapid evolution, with unpredictable consequences for human and animal health (VAZ-MOREIRA; NUNES; MANAIA, 2014).…”

Section: Introductionmentioning

confidence: 99%

Biofilm forming antimicrobial-multiresistant Staphylococcus aureus

Bohrz¹,

Webber²,

Pereira³

et al. 2021

AVB

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Staphylococcus aureus is one of the main agents isolated from bovine mastitis cases, characterized by lower cure rates compared to other pathogens causing this disease. This phenomenon is mainly explained by the multiresistance acquisition to antimicrobials and the ability of S. aureus to form biofilms on biotic and abiotic surfaces. In this work 15 samples of S. aureus isolated from the automated milking facility were analyzed regarding the resistance profile to antimicrobials, virulence factors (capsule production, hemolysin, and protease) and adhesion capacity under different temperatures (42±1°C, 36±1°C, 25±1°C, 9±1°C, and 3±1°C). All isolates showed methicillin-resistant (MRSA) characteristics and multidrug resistance profile to the antimicrobials tested (penicillin G, chloramphenicol, oxacillin, cephalexin, tetracycline, amoxicillin + clavulanic acid, sulfa + trimetropim, gentamicin, doxycycline, ceftiofur, neomycin, and vancomycin) with an IRMA index between 0.5 and 1.0. Five isolates were resistant to vancomycin (VRSA), two were resistant to all active principles, and the others to at least six of these drugs. Adhesion capacity and biofilm formation were found in 3 of the 5 evaluated temperatures, including the cooling conditions. Regarding the virulence factors, 86.7% of the isolates formed capsules, 60% revealed the presence of protease, 26.7% expressed the α-hemolysin factor, and 13.3% of them presented β-hemolysin. The fact that all isolates presented MRSA characteristics represents a potential risk to those exposed to this agent, and the formation of biofilm in liners even after the use of detergents and sanitization highlights the urgency of searching for alternatives for dispersion of the biofilm by S. aureusin the automated milking facility.

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Prevalence and characterization of Staphylococcus aureus isolates from subclinical bovine mastitis in southern Xinjiang, China

Cited by 79 publications

References 46 publications

Update on molecular diversity and multipathogenicity of staphylococcal superantigen toxins

Update on molecular diversity and multipathogenicity of staphylococcal superantigen toxins

Microbiological Evaluation of Milk Quality and Antibiogram in Dairy Cows Managed on Pasture

Biofilm forming antimicrobial-multiresistant Staphylococcus aureus

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