Prevalence and genomic characteristics of canine kobuvirus in southwest China

Li, Mingxiang; Yan, Nan; Wang, Min; Zhang, Bin; Yue, Hua; Tang, Cheng

doi:10.1007/s00705-017-3648-y

Cited by 16 publications

(18 citation statements)

References 18 publications

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“…The CaKoV-positive rate observed in this study (16.13%) was similar to those reported previously in diarrheic dogs in northeast China (17.91%) [17], South Korea (19.0%) [25], and Thailand (17.59%) [18] and lower than those reported in southwest China (50.46%) [22] and Japan (37.2%) [15]. The CaKoV-positive rates in diarrheic dogs observed in the United States, Italy, and Brazil were lower (3%, 4.4%, and 5.7%, respectively) than that observed in our study [4,12,19].…”

Section: Discussionsupporting

confidence: 90%

“…These data indicate that CaKoVpositive rates may differ between different countries or regions. Some studies have found CaKoV not only in dogs with gastroenteritis but also in healthy animals [15,18,22,25]. In this study, CaKoV was detected in 20% (4/20) of dogs without diarrhea symptoms.…”

Section: Discussionsupporting

confidence: 44%

“…All fecal samples were tested for canine parvovirus 2 (CPV-2), canine coronavirus (CCoV), and CaKoV by PCR or RT-PCR according to previously described procedures [11,[20][21][22]. The purified amplification products of the CaKoV 3D gene fragment and the VP1 gene were ligated into a Blunt-Zero cloning vector using a 5 min TA/Blunt-Zero Cloning Kit (Vazyme Biotech Co., Ltd., Nanjing, China).…”

Section: Detection Of Pathogensmentioning

confidence: 99%

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Epidemiological and phylogenetic analysis of canine kobuviruses in Tangshan, China

et al. 2020

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Canine kobuviruses (CaKoV) have been found in healthy and diarrheic dogs as well as asymptomatic wild carnivores in various countries. In order to investigate the prevalence and evolution of CaKoV in Tangshan, China, 82 dog fecal samples from pet hospitals in Tangshan were subjected to RT-PCR targeting a segment of the 3D gene of CaKoV. Using this method, we identified CaKoV in 14 samples (17.07%, 14/82). Of the CaKoV-positive samples, 78.57% (11/14) and 50% (7/14) were positive for canine parvovirus and canine coronavirus, respectively. The nucleotide sequences of the 14 strains 96.6%-100% identical to each other and 77.6%-99.2% identical to representative sequences from the NCBI GenBank database. We also amplified the 14 VP1 gene sequences and found that they were 93.3%-99.6% identical to each other and 73.3%-97.8% identical to representative sequences from the NCBI GenBank database. Phylogenetic analysis revealed that the 14 CaKoV strains from Tangshan are closely related to those identified in China and Thailand and display less similarity to those found in Africa, the United States, and Europe. Our data suggest that CaKoV circulated in young pet dogs in Tangshan and displays a high co-infection rate with CCoV and CPV. However, the relationship between the three viruses and their roles in the host requires further investigation.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 44%

Section: Detection Of Pathogensmentioning

confidence: 99%

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Epidemiological and phylogenetic analysis of canine kobuviruses in Tangshan, China

et al. 2020

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“…The first described canine circovirus case had severe necrotising vasculitis and granulomatous disease, as well as haemorrhagic gastroenteritis. Li et al (2013a) examined histological samples from dogs with similar pathological findings and were able to identify a further Kong et al (2016) 4.7% a 0% Li et al (2017) 50.5% NP Korea Oem et al (2014) 19% 13.2% Choi et al (2014 50.6% NP Japan Soma et al (2016) 37.2% 48.0%…”

Section: Epidemiology and Pathogenesismentioning

confidence: 99%

New viruses associated with canine gastroenteritis

Caddy

2018

The Veterinary Journal

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A number of novel viruses have been associated with canine gastroenteritis in recent years, from viral families as diverse as Caliciviridae and Picornaviridae to Parvoviridae and Circoviridae. The ability of many of these viruses to cause disease is uncertain, but epidemiological studies are continually adding to our knowledge of these potential pathogens. This review presents a summary of the latest research and current understanding of novel viruses associated with canine gastroenteritis.

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“…The structural proteins form the capsid, which is associated with the adsorption and entry of virus particles into cells. Based on the sequence of the VP1 gene, virus strains can be divided into two groups [12]. The non-structural proteins and intermediates are involved in RNA replication, polyprotein cleavage, and virion assembly [9].…”

Section: Introductionmentioning

confidence: 99%

Identification and full-genome sequencing of canine kobuvirus in canine fecal samples collected from Anhui Province, eastern China

Wang

Cui

et al. 2020

Arch Virol

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Canine kobuvirus (CaKoV), a newly described virus, is the causative agent of gastroenteritis in dogs. In this study, 57 fecal samples from dogs with diarrhea in Anhui Province, eastern China, were collected. Among these, five samples were identified to be infected with CaKoV, by polymerase chain reaction targeting the CaKoV 3D gene. The five CaKoV strains were subjected to phylogenetic analysis. The sequences of VP1 from the five CaKoV strains were 93.6%-96.1% identical to each other and 91.75%-97.95% identical to other reported CaKoV VP1 sequences. In addition, the complete genome of one strain was successfully amplified and sequenced. The genome consisted of 8223 nucleotides and shared 94.6%-97.0% nucleotide and 93.1%-94.0% amino acid sequence identity with other CaKoV isolates. Phylogenetic analysis revealed that the CaKoV strain from Anhui Province was similar to other Chinese strains, and it was more closely related to feline and mouse kobuviruses than to sheep and bovine kobuviruses. Interestingly, all of the CaKoV-positive samples were coinfected with canine parvovirus. The finding of CaKoV infection in dogs with diarrhea and coinfection with canine parvovirus are a cause for concern and highlight the need for management and preventive measures.

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Prevalence and genomic characteristics of canine kobuvirus in southwest China

Cited by 16 publications

References 18 publications

Epidemiological and phylogenetic analysis of canine kobuviruses in Tangshan, China

Epidemiological and phylogenetic analysis of canine kobuviruses in Tangshan, China

New viruses associated with canine gastroenteritis

Identification and full-genome sequencing of canine kobuvirus in canine fecal samples collected from Anhui Province, eastern China

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