Prevalence and Genotypes of
            <i>Helicobacter pylori</i>
            in Gastric Biopsy Specimens from Patients with Gastroduodenal Pathologies in the Cukurova Region of Turkey

Nağıyev, Toğrul; Yula, Erkan; Abaylı, Bahri; Köksal, Fatih

doi:10.1128/jcm.00605-09

Cited by 22 publications

(24 citation statements)

References 26 publications

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“…No s2m1 genotype was found in this study. This finding correlated confirm few data reported from other geographic regions of Turkey (6,10,18).…”

Section: H Pylori Genotypessupporting

confidence: 83%

“…Our data similar to previous reports in Turkey where vacAs1a strains were showed to be significantly associated with PU (10,18), but different from a study in Turkey reported that vacAs1a strains were not found to be statistically associated with PU (26).…”

Section: H Pylori Genotypessupporting

confidence: 81%

“…Our results supported the findings of the studies performed in Turkey and other countries (3,6,10,18) where the s1m2 genotype was the most common gene combinations of the vacA, but contrary to studies (8,26) reported that s1m1 genotype was the most predominant gene. The prevalence of s2m2 genotype was determined as 11.4% and all of s2m2 positive isolates were cagA negative.…”

Section: H Pylori Genotypessupporting

confidence: 68%

“…We detected a low prevalence of vacAs1b in this study which was contrast to pevious reports in Portugal, Central and South America (32). No vacAs1c genotype was determined except for the only one study reported in Turkey (18). In this study, the prevalence of the vacAs1c gene was found to be low.…”

Section: H Pylori Genotypescontrasting

confidence: 55%

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Prevalence of genotypes in Helicobacter pylori isolates from patients in eastern Turkey and the association of these genotypes with clinical outcome

Ozbey¹,

Aygün²

2012

Braz. J. Microbiol.

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There is not much information available regarding the prevalence of the genotypes of Helicobacter pylori isolates in Turkey, particularly in eastern Turkey. The aims of this study were to detect the prevalence of different genotypes of H. pylori in Turkish patients with gastrointestinal complaints and to determine the relationship of these genotypes with clinical outcome and sex. One hundred forty H. pylori isolates were examined for the presence of its genotypes by the PCR. We found that the prevalence of vacAs1, vacAs2, cagA, cagE, iceA1, iceA2 and babA2 genes were 88.6%, 11.4%, 71.4%, 35.7%, 41.4%, 58.6%, and 62.1%, respectively. The most predominant vacA subtype was s1a (81.4%). The most vacA allelic combination detected were vacAs1m1 (65.2%) and s1m2 (53.9%) in patients with peptic ulcer and gastritis, respectively.The only vacAs1 isolate was significantly associated with gastritis and peptic ulcer (p<0.05). The vacAs1a, ml, slml and babA2 genes were significantly associated with peptic ulcer (p<0.05), whereas m2 gene was significantly associated with only gastritis (p<0.05). The difference between sex and genotypes was statistically significant among the cagA, vacAs1, iceA2 and babA2 genes. This study reported for the first time the prevalence of H. pylori genotypes in patients with gastrointestinal complaints in eastern Turkey.Further studies are needed to understand epidemiological importance of the genotypes of H. pylori isolates in this region and the association between the virulence genes and clinical outcome in different regions.

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“…No s2m1 genotype was found in this study. This finding correlated confirm few data reported from other geographic regions of Turkey (6,10,18).…”

Section: H Pylori Genotypessupporting

confidence: 83%

Section: H Pylori Genotypessupporting

confidence: 81%

Section: H Pylori Genotypessupporting

confidence: 68%

Section: H Pylori Genotypescontrasting

confidence: 55%

See 2 more Smart Citations

Prevalence of genotypes in Helicobacter pylori isolates from patients in eastern Turkey and the association of these genotypes with clinical outcome

Ozbey¹,

Aygün²

2012

Braz. J. Microbiol.

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“…For example, Yamaoka et al (1999Yamaoka et al ( , 2002 (Erzin et al, 2006;Nagiyev et al, 2009;Kolaylı et al, 2012;Ozbey et al, 2013;Yula et al, 2013). Similarly, our Cag A positivity rate (83.5 %) for H. pylori strains was in accordance with previous Turkish studies.…”

Section: Epiya-c and -D Have A Role In The Interaction Betweensupporting

confidence: 81%

Patterns of EPIYA motifs among cagA-positive Helicobacter pylori strains: a case–control study in a Turkish population with Eurasian geographical features

Kocazeybek

Caliskan

Cetin

et al. 2015

Journal of Medical Microbiology

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Geographical variation in the frequency of various gastroduodenal pathologies was shown to be related to the geographical diversity of H. pylori CagA Glu-Pro-Ile-Tyr-Ala (EPIYA) patterns. We examined the EPIYA patterns of H. pylori and the association of EPIYA patterns with gastric cancer (GC) for the first time, to the best of our knowledge, in Turkey. The patient group (PG) contained 60 patients [38 GC and 22 duodenal ulcer (DU) patients]. The control group (CG) was 110 individuals [94 gastritis patients and 16 persons with a normal gastrointestinal system (NGIS)]. Specific primers were used for the detection of cagA including empty-site-positive and EPIYA-A, -B, -C, -D PCR. Bands of EPIYA-A, -B, -C were confirmed by DNA sequencing. One hundred and forty-two (83.5 %) strains [60 in the PG (38 GC, 22 DU), 82 in the CG (72 gastritis, 10 NGIS)] were positive for the cagA gene. EPIYA-C with multiple repeats was detected in 34 (23.9 %) strains, and 22 (64.7 %) were from GC patients. EPIYA-C with one repeat was detected in 89 (62.7 %) strains, and 54 (60.7 %) were from gastritis patients. EPIYT was detected in 10 strains, and EPIYA-D was not detected. The number of EPIYA-C with multiple repeats was significantly higher for the PG than for the CG (P,0.0001). In GC patients, the number of EPIYA-C with multiple repeats was significantly higher than one repeat (P,0.0001). In conclusion, our study showed that multiple EPIYA-C repeats increases the GC risk by 30.6-fold and the DU risk by 8.9-fold versus the CG. This indicates that Western-type H. pylori strains in Turkey have similar EPIYA motifs to those of neighbouring countries and Western populations. INTRODUCTIONHelicobacter pylori is the principal cause of gastritis and gastric or duodenal ulcer (DU) diseases and is involved in the development of gastric cancer (GC) and mucosaassociated lymphoid tissue lymphoma (Atherton, 2006).H. pylori is a class 1 carcinogen identified by the International Agency for Research on Cancer for chronic and persistent infections. It is resistant to antibacterial agents in spite of innate and adaptive immunity (International Agency for Research on Cancer, 1994). Depending on virulence factors such as cytotoxin-associated gene A (CagA), blood group antigen-binding adhesion (BabA2) and vacuolating cytotoxin gene A (VacA), different genotypes cause different pathological and clinical outcomes and geographical regional differences (Atherton et al., 1995;Basso et al., 2008). Recently, the Glu-Pro-Ile-Tyr-Ala (EPIYA)Abbreviations: cagPAI, cag pathogenicity island; CG, control group; CI, confidence interval; DU, duodenal ulcer; EPIYA, Glu-Pro-Ile-Tyr-Ala; CagA, cytotoxin-associated gene A; GC, gastric cancer; NGIS, normal gastrointestinal system; PG, patient group; OR, odds ratio. pattern of CagA was suggested to be involved in the pathogenesis of GC, as this pattern manifests important variations depending on the region (Argent et al., 2004(Argent et al., , 2005.The CagA protein is injected into host epithelial cells with its peptidoglycan b...

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Association between vacA genotypes and the risk of duodenal ulcer: a meta-analysis

Zhang

Liu³

et al. 2014

Mol Biol Rep

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Epidemiological studies have reported the relationship between vacuolating cytotoxin A (vacA) s-/m- region genotypes and duodenal ulcer (DU), but the results remained inconclusive. We performed the present meta-analysis to investigate a more authentic association between vacA s-/m- region genotypes and DU. Literature search was performed by searching Embase, PubMed and ISI Web of Science databases as well as checking references from identified articles, reviews and the abstracts presented at related scientific societies meetings. The association was assessed by combined odds ratio (OR) with 95% confidence interval (CI). A total of 42 studies were included in our final meta-analysis. The combined ORs (95% CIs) showed that vacA s1 (OR = 2.96, 95% CI = 2.34-3.75), m1 (OR = 1.46, 95% CI = 1.05-2.04) and s1m1 (OR = 1.89, 95% CI = 1.47-2.42) were associated with increased DU risk significantly in the overall studied population. Subgroup analyses by ethnicity showed that vacA s1 increased the risk of DU in Asian countries (OR = 1.92, 95% CI = 1.30-2.83), European countries (OR = 3.58, 95% CI = 2.13-6.03) and Latin American countries (OR = 4.20, 95% CI = 2.21-7.98); vacA m1 increased the risk of DU in Latin American countries (OR = 2.98, 95% CI = 1.59-5.56); vacA s1m1 increased the risk of DU in Asian countries (OR = 2.04, 95% CI = 1.12-3.73) and Latin American countries (OR = 2.05, 95% CI = 1.20-3.48); vacA s2m1 increased the risk of DU in Latin American countries (OR = 2.30, 95% CI = 1.17-4.50). The data suggest that genotype testing of vacA s- and m- region will be useful in screening susceptible individuals for DU development.

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Prevalence and Genotypes of Helicobacter pylori in Gastric Biopsy Specimens from Patients with Gastroduodenal Pathologies in the Cukurova Region of Turkey

Cited by 22 publications

References 26 publications

Prevalence of genotypes in Helicobacter pylori isolates from patients in eastern Turkey and the association of these genotypes with clinical outcome

Prevalence of genotypes in Helicobacter pylori isolates from patients in eastern Turkey and the association of these genotypes with clinical outcome

Patterns of EPIYA motifs among cagA-positive Helicobacter pylori strains: a case–control study in a Turkish population with Eurasian geographical features

Association between vacA genotypes and the risk of duodenal ulcer: a meta-analysis

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