Prevalence and haemato-biochemical profile of Anaplasma marginale infection in dairy animals of Punjab (India)

Ashuma,; Sharma, Anil Kumar; Singla, L. D.; Kaur, Paramjit; Bal, Mandeep Singh; Batth, Balwinder Kaur; Juyal, P. D.

doi:10.1016/s1995-7645(13)60010-3

Cited by 61 publications

(40 citation statements)

References 17 publications

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“…[13], whereas Ashuma et al . [14] found prevalence of anaplasmosis to be on higher side (11.25%) as compared to the present study (4.17%). As there was no previous report of anaplasmosis from TVCC, Hisar, Haryana, in the recent past, so the disease can be categorized under the emerging status.…”

Section: Discussioncontrasting

confidence: 71%

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Emerging status of anaplasmosis in cattle in Hisar

Kumar¹,

Sindhu²,

Charaya³

et al. 2015

Vet World

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Aim:Anaplasma marginale is a rickettsial pathogen responsible for progressive anemia in ruminants leading to huge economic losses. The objectives of this cross-sectional study were to determine the prevalence of anaplasmosis and therapeutic evaluation of traditional line of treatment.Materials and Methods:A total of 168 cattle presented to Teaching Veterinary Clinical Complex, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar during the period of 3 months (July-September, 2014) with history of fever, anorexia, reduced milk yield and tick infestation were analyzed for prevalence of hemoprotozoan diseases using classical giemsa stained thin blood smear parasitological method.Results:Out of these 168 animals, 7 (4.17%) were found to be suffering from anaplasmosis on the basis of presence of dense, rounded, intra-erythrocytic bodies situated on or near the margin of the erythrocytes. Overall prevalence of theileriosis and babesiosis were found to be 42.9% and 1.8%, respectively. Level of parasitemia was noticed to be 1.2%, 0.8% and 0.9% in babesiosis, theileriosis, and anaplasmosis, respectively. The most marked and common clinical signs reported in all the cases were severe anemia (hemoglobin=3-6 g/dl) and history of fever, followed by normal body temperature. Following treatment with oxytetracycline parenterally along with supportive therapy out of seven cases six got recovered without any side-effects.Conclusion:The current study indicates the emerging status of anaplasmosis in this part of the country as during the past few years there are very few reports showing the prevalence of clinical cases of anaplasmosis. Treatment with oxytetracycline yielded excellent result showing recovery in most of the clinical cases.

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Section: Discussioncontrasting

confidence: 71%

“…Level of parasitemia (5.9%) shown by Ashuma et al . [14] was found to be on higher side as compared to parasitemia in the present study (0.9%). There was no characteristic difference in the level of parasitemia and clinical manifestation.…”

Section: Discussioncontrasting

confidence: 42%

Emerging status of anaplasmosis in cattle in Hisar

Kumar¹,

Sindhu²,

Charaya³

et al. 2015

Vet World

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“…It was found that the calves were 3.62 and 2.53 times more susceptible when compared to adult cows for A. marginale and Babesia sp., respectively. Similar results were observed for A. marginale by Ashuma et al (2013). The results of this paper disagree with Terkawi et al (2012) that found greater positivity of Babesia in adult cattle.…”

Section: Discussioncontrasting

confidence: 58%

Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, Brazil

Amorim

Wenceslau

Carvalho

et al. 2014

Rev. Bras. Parasitol. Vet.

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Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, BrazilComplexo tristeza parasitária bovina: diagnóstico e avaliação dos fatores de risco na Bahia, Brasil AbstractDirect diagnoses were made by using -blood smears and nested PCR (nPCR) tests on 309 blood samples from crossbred dairy cattle in the municipality of Ibicaraí, Bahia. From diagnostic blood smear slides, the observed parasitic frequencies were 31.1% for Anaplasma marginale and 20.4% for Babesia sp. From nPCR diagnoses, they were 63% for A. marginale, 34% for Babesia bigemina and 20.4% for Babesia bovis. There were significant differences (P < 0.01) between the two diagnostic methods (nPCR and blood smear slides). The compliance obtained from the kappa test was 0.41 and 0.48 for A. marginale and Babesia sp., respectively. The tick samples from the six farms analyzed using nPCR were only positive for A. marginale. Evaluation of the risk factors relating to the presence of ticks and the age of the animals showed that there was a significant association (P < 0.01) with the frequency of animals infected with both pathogens. Therefore, under the conditions studied, nPCR proved to be a good tool for diagnosing the agents of the bovine babesiosis and anaplasmosis complex because of its sensitivity and specificity in comparison with blood smears. The municipality of Ibicaraí is an area with endemic prevalence of bovine babesiosis and anaplasmosis confirmed by nPCR and A. marginale is the main agent of the disease.Keywords: Bovine Babesiosis and Anaplasmosis Complex, blood smear, nPCR, risk factors. ResumoRealizou-se o diagnóstico direto por esfregaço sanguíneo e nested PCR (nPCR) em 309 amostras de sangue de bovinos mestiços leiteiros provenientes do município de Ibicaraí, Bahia. A frequência observada no diagnóstico por lâminas de esfregaço sanguíneo foi 31,1% para Anaplasma marginale e 20,4% para Babesia sp. Enquanto que no diagnóstico por nPCR foi 63% para A. marginale, 34% para Babesia bigemina e 20,4% Babesia bovis. Verificaram-se diferenças significativas (P<0,01) na comparação entre os dois métodos de diagnósticos (nPCR e esfregaço sanguíneo). A concordância ao teste KAPPA obtida foi de 0,41 e 0,48 para A. marginale e Babesia sp., respectivamente. As amostras de carrapatos das seis propriedades analisadas por nPCR foram positivas apenas para A. marginale. Na avaliação dos fatores de risco verificou-se que a presença de carrapato e idade dos animais apresentaram associação significativa (P<0,01) com a frequência de animais infectados por ambos os patógenos analisados por nPCR. Portanto, nas condições estudadas, a nPCR revelou-se uma boa ferramenta para diagnóstico dos agentes do complexo tristeza parasitária bovina (TPB) devido a sensibilidade e especificidade, quando comparado ao esfregaço sanguíneo. O município de Ibicaraí

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“…Bovine anaplasmosis is a severe disease of cattle, characterised by haemolytic anaemia and causes significant economic loss (Ashuma et al, 2013). The losses are as a result of reduction in milk production, increased cost of treatment and management of the disease condition.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%