Prevalence and multilocus sequence typing of Clostridium perfringens isolated from 4 duck farms in Shandong province, China

Li, Xiu; Liu, Yu; Wu, Wei; Suo, Chen; Zhong, Zhijun; Wang, Hairong

doi:10.1016/j.psj.2020.06.046

Cited by 27 publications

(16 citation statements)

References 42 publications

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“…Other studies conducted in India captured higher prevalence as 33.89% in poultry feed samples in Tamil Nadu, South India [ 37 ], 33.63% and 18.91% in broiler of 2-6 weeks age and older layer, respectively, in Kashmir, India [ 38 ], and 53% dead broilers in West Bengal, India [ 39 ]. The prevalence that was established from this study is comparatively lower than the earlier studies conducted in different geographical locations 48.82% [ 40 ] and 23.1% [ 41 ] in China; 29.6% in Taiwan [ 42 ]; 57.9% in Egypt [ 29 ]; and 24.72% and 23.28% from two commercial poultry farms in Canada [ 43 ]. However, we could not compare our study findings in a country context due to the lack of reference data of NE in broiler chicken using the advance molecular assay.…”

Section: Discussioncontrasting

confidence: 65%

Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

et al. 2021

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Background and Aim: Necrotic enteritis (NE) is one of the most prevalent diseases in broiler poultry caused by Clostridium perfringens connected with significant economic losses. A cross-sectional survey was conducted in Mymensingh district of Bangladesh to assess the prevalence of C. perfringens through toxinotyping molecular assay and confirm the risk factors for NE, including antimicrobial-resistant (AMR) status of the isolates. Materials and Methods: We included 40 small-scale commercial broiler farms randomly selected from two subdistricts of Mymensingh district of Bangladesh. As an individual sample, 240 cloacal swabs, and as a pooled sample, 40 drinking water, 40 workers' hand washing, 40 litter swab, and 40 feed samples were collected and evaluated by culture, biochemical, and molecular assays. A pretested semi-structured interview questionnaire was employed to capture flock-level data on risk factors from the farm owners. The flock-level data on risk factors were assessed through univariable and multivariable logistic regression analyses with p<0.05 was considered statistically significant. Results: Overall flock-level prevalence of C. perfringens was estimated to be 10.3% (95% confidence interval [CI] 7.5- 13.6%). Litter swab (pooled) was found to be highly contaminated with C. perfringens (25.0%, 95% CI: 12.7-41.2%) followed by the cloacal swab (10.4%, 95% CI: 6.9-15.0%) and feed sample (5.0%, 95% CI: 0.6-16.9%). History of coccidia infection (Adjusted odds ratio =33.01, 95% CI: 2.14-507.59, p=0.01) was significantly associated with flock-level C. perfringens infection status. In this study, 78.1% isolates were found as multidrug-resistant as they demonstrated resistance to 3-5 antimicrobial agents. Conclusion: Evidence-based control options need to be taken through the uses of prebiotics and probiotics, biosecurity, and hygienic measurement, including control of coccidia infection, is needed to lessen the NE infection and AMR related to this pathogen in small-scale commercial broiler poultry.

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Section: Discussioncontrasting

confidence: 65%

Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

et al. 2021

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“…p < .05 was considered statistically significant and used for variance analysis of antibiotic resistance rate (Xiu et al, 2020). Sequence analysis and ST determinations of CCs were performed using a self-built database for ST designation.…”

Section: Discussionmentioning

confidence: 99%

“…Not only C. perfringens, but also other bacteria isolated in Shandong, such as Escherichia coli, have developed resistance to a variety of antibiotics (Song et al, 2020). The spread of multiple antimicrobial resistance poses a major threat to public health (Xiu et al, 2020), and furthermore, antibiotic residues and food safety problems have hindered the development of the poultry industry in China (Guo et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Tracing Clostridium perfringens strains along the chicken production chain from farm to slaughter by multilocus sequence typing

Wang

Suo

et al. 2021

Zoonoses and Public Health

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The current study is undertaken to characterize the prevalence, genotypes distribution, antibiotic resistance and genetic diversity of Clostridium perfringens (C. perfringens) collected from different stages of a chicken production chain. In total, 579 samples from a broiler farm and 378 samples from the slaughterhouse were collected from a large-scale rearing and slaughter one-stop enterprise in Weifang, China, between June and July 2019, of which 30.40% of the samples from farm and 54.50% of samples from slaughterhouse were determined to be positive for C. perfringens, respectively. The contamination of chicken products was relatively serious, with the total positive rate of carcasses at 59.73%; the positive rate of carcass samples was the highest in the evisceration process, which might be the critical point of C. perfringens contamination. A total of 476 isolates of C. perfringens were recovered; and 99.58% of recovered isolates were identified as type A, with the remaining isolates being type G.Antimicrobial susceptibility testing revealed that 97.15% of the isolates showed multiple antibiotic resistance and 67.89% of them were resistant to at least five classes of commonly used antibiotics. Multilocus sequence typing results of 91 representative isolates showed that the isolates can be divided into 74 sequences types (STs); 40.66% of the isolates can be included into seven clonal complexes (CCs). Although most of the isolates were classified as type A, considerable genetic diversity was observed, with the Simpson's diversity index of ST up to 0.9902. Some isolates from farm stage and slaughter stage were distributed in the same ST or CC, indicating that chicken products may be contaminated by the same ST or CC of C. perfringens originated from the farm stage. The high contamination rates of chicken products and the widespread multiple antibiotic resistance of isolates indicated potential public health risks, control measures at rearing and slaughtering stage should be considered to reduce this risk.

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“…Food-producing animals, especially poultry, serve as resistance gene “reservoirs” ( Liu et al., 2017 ; Song et al, 2020b ; Xiu et al, 2020 ). Alarmingly, spread of the plasmid-mediated POL resistance mcr-1 gene into NDM-producing bacterial isolates can cause untreatable infections ( Xu et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of avian-origin mcr-1–positive Escherichia coli with a potential risk to humans in Tai'an, China

Lin

et al. 2020

Poultry Science

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Multidrug-resistant ( MDR ) Escherichia coli are responsible for difficult-to-treat infections. We sought to determine the prevalence and characteristics of MDR E. coli strains isolated from poultry and clinical patients in the same geographical region. Eighty-seven E. coli strains were isolated from poultry with perihepatitis lesions at different slaughterhouses, and 356 nonrepetitive E. coli strains were isolated from clinical patients. All samples were continuously collected from October to December 2017 in Tai'an, China. The presence of the mcr-1 gene in the strains was assessed by PCR. The genetic relationships of the polymyxin ( POL )-resistant E. coli strains were analyzed by pulsed-field gel electrophoresis and multilocus sequence typing. The results indicate that the POL resistance rate for the E. coli isolates from poultry was 31.03% (27 of 87), whereas the human-origin E. coli isolates were 100% sensitive to POL. The mcr-1 gene and extended-spectrum β-lactamase bla CTX-M-14 genes were identified in all 27 POL-resistant avian-origin E. coli isolates. Our pulsed-field gel electrophoresis analysis suggested that the 27 strains were represented by 14 pulsotypes, among which there were 3 strains each with A, E, I, and K pulsotypes, and 1 to 2 strains represented by the other 10 pulsotypes. Furthermore, multilocus sequence typing molecular typing identified 16 sequence types, including 4 ST156 strains, 3 ST533 strains, and 1 to 2 strains represented by the remaining 14 sequence types. In summary, the E. coli strains isolated in the Tai'an area all showed the MDR phenotype, the rate of which for poultry was higher than that for humans. No POL-resistant human-origin E. coli strains were identified in the clinical patients. Our study reveals that poultry-derived MDR mcr-1 –positive E. coli strains may pose a potential risk to humans, and the surveillance findings presented herein will be conducive to our understanding of the prevalence and characteristics of mcr-1 –positive E. coli strains in the Tai'an area.

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Prevalence and multilocus sequence typing of Clostridium perfringens isolated from 4 duck farms in Shandong province, China

Cited by 27 publications

References 42 publications

Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

Tracing Clostridium perfringens strains along the chicken production chain from farm to slaughter by multilocus sequence typing

Prevalence of avian-origin mcr-1–positive Escherichia coli with a potential risk to humans in Tai'an, China

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