Prevalence and specificity of red‐blood‐cell antibodies in a multiethnic South and East Asian patient population and influence of using novel MUT+Mur+ kodecytes on its detection

Abstract

Antibodies to vMNS antigens are common in South and East Asian populations and are often missed when using standard screening cells. Use of specifically engineered screening cells to express red-cell antigens artificially is beneficial in detecting the diverse alloantibodies present in our population.

MNS Blood Group System

“…Antibodies to GP.Mur red cells (probably mainly anti -Mur, but often called anti -' Mi a ' ) have been responsible for immediate and delayed HTRs [332,333] and severe HDFN [333 -335] (reviewed in [336] ). Antibodies to GP.Mur cells are among the most common atypical alloantibodies detected in eastern Asia [219,220,322,337,338] . They often have an IgM component; of those that contain IgG, it is almost always IgG1 and/or IgG3, and of those containing IgG, 69% were reactive in a monocyte monolayer functional assay [339] .…”

“…The DRB1 * 0901 allele frequency was signifi cantly higher in patients with anti -' Mi a ' than in a control group [340] . Red cells resembling GP.Mur cells have been synthesised by embedding appropriate peptides attached to lipids in the membrane of red cells of common phenotype [337,341] . Red cells resembling GP.Mur cells have been synthesised by embedding appropriate peptides attached to lipids in the membrane of red cells of common phenotype [337,341] .…”

The prevalence, immunogenicity, and evanescence of alloantibodies to MUT and Mur antigens of GP.Mur red blood cells in a Southeast Asian patient cohort

“…Antibodies against these antigens are often the most commonly reported RBC antibodies among patient populations in Southeast and East Asia . The actual prevalence of these antibodies in affected Asian populations is not clear, however, in view of the lack of a uniform policy for antibody screening or usage of relevant reagent cells for their detection.…”

“…The peptide sequence TYPAHTANE corresponds to Mur and SQTNDKHKRDT to MUT. Using these screening cells, we have previously shown that anti‐Mur and anti‐MUT can be separately identified . Anti‐Mi a is however not identified using these cells, as they have not been engineered to express the Mi a antigen.…”

Designing peptide‐based FSL constructs to create Miltenberger kodecytes

“…3. Initial developmental testing [8] and later field testing [51,52] over the last few years have established that MUT kodecytes will detect both anti-MUT and anti-Mi a while…”