Prevalence of AmpC β-lactamase in Clinical Isolates of Escherichia coli, Klebsiella spp., and Proteus mirabilis in a Tertiary Hospital in Tehran, Iran

Saffar, Hiva; Niaraki, Neda Asgari; Tali, Arash Ghahroudi; Baseri, Zohre; Abdollahi, Alireza; Yalfani, Roozbeh

doi:10.5812/jjm.39121

Cited by 12 publications

(17 citation statements)

References 13 publications

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“…Also, according to the PCR assay results, we found the prevalence of AmpC genes in 35.3% AmpCproducing isolates. The result was consistent with those of the studies carried by Saffar et al (5) and Fam et al (26) from Egypt where AmpC prevalence was 15.83% and 28.3%, respectively. On the other hand, the base on the geographical location, the species studied, the period of study, the prevalence, and type of acquired plasmid AmpC detected can be different(27).…”

Section: Discussionsupporting

confidence: 93%

“…Importantly, Amp C is a major clinical concern since these enzymes play a signi cant role in conferring cephamycins hydrolyze and also are able to increase the minimum inhibitory concentration (MIC) value of carbapenems (3,4). ESBLs and AmpC βlactamases production can be chromosomal or plasmid-mediated and can be spread by horizontal transfer between gram-negative bacteria members (5,6).…”

Section: Introductionmentioning

confidence: 99%

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The prevalence of ESBL and AmpC β-lactamases in uropathogenic isolates of Escherichia coli in a tertiary care hospital in Southwest Iran

et al. 2020

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Objectives: The spread of extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamases (AmpC) in Escherichia coli (E. coli) is an important public health concern and ESBL-producing bacteria are commonly reported in uropathogenic Escherichia coli (UPEC). The aim of this study is to determine the molecular detection of AmpC and ESBLs, among clinical E. coli isolated from inpatients who presented with symptomatic urinary tract infection (UTI) in Shiraz, the southwest of Iran. Results: Among total 177 urinary isolates, the analysis showed that 46.3% of the isolates were ESBLs positive and that fteen strains revealed the AmpC cluster genes. Among all ESBL-positive E. coli isolates, the CTX-M was the most prevalent ESBL type (68.2%), and in AmpC-positive isolates, fteen isolates (88.2%) were positive for CITM cluster gene and two (11.7%) were positive for DHAM cluster gene. ACCM, FOXM , EBCM and MOXM cluster genes were not found in this study. Our ndings revealed that the prevalence of AmpC β-lactamases is rising in Iran, leading to failure in treatment. Therefore, the current study recommended that accurate and precise methods and guidelines should be designed for detection of antibiotic-resistant mechanisms because it is very important for the treatment and prevention of such isolates.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

The prevalence of ESBL and AmpC β-lactamases in uropathogenic isolates of Escherichia coli in a tertiary care hospital in Southwest Iran

et al. 2020

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“…They reported that 13 (3.3%) isolates were identified as AmpC producers, which 11 of the 13 isolates contained the bla CMY gene. In another survey performed by Saffar et al [24] in Iran (Tehran), bla DHA gene was detected in 14.8% isolates of E. coli. Their results confirmed this hypothesis that geographical location plays the most important role in the distribution of bla DHA gene, due to the closer regions have a relatively similar distribution of bla DHA gene [24].…”

Section: Discussionmentioning

confidence: 92%

“…In another survey performed by Saffar et al [24] in Iran (Tehran), bla DHA gene was detected in 14.8% isolates of E. coli. Their results confirmed this hypothesis that geographical location plays the most important role in the distribution of bla DHA gene, due to the closer regions have a relatively similar distribution of bla DHA gene [24]. Recently, plasmid-mediated quinolone resistance determinants have been also reported among E. coli isolates, furthermore, plasmid-mediated fosfomycin-modifying enzymes, fosA3 and fosC2, were identified in E. coli clinical isolates.…”

Section: Discussionmentioning

confidence: 92%

Emergence of fosfomycin resistance among isolates of Escherichia coli harboring extended-spectrum and AmpC β-lactamases

Bahramian

Eslami

Hashemi

et al. 2017

AMicr

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Urinary tract infection (UTI) is a common type of infectious disease globally. The aim of this study was to detect the frequency of fosA3 and fosC2 genes in extended-spectrum β-lactamases (ESBL) and bla, bla, and bla genes in AmpC β-lactamases-producing isolates of Escherichia coli. In total, 120 isolates of E. coli were collected from three teaching hospitals between March 2014 and February 2015. Antibiotic susceptibility tests were carried out by disk diffusion method. The presence of bla, bla, bla, fosA3, and fosC2 genes was detected by polymerase chain reaction (PCR) and sequencing. Of the 120 strains, 92 (76.6%) were identified as ESBL producers, 30 (25%) were determined as AmpC β-lactamase producers, and 24 (20%) had both ESBL and AmpC β-lactamase enzymes. Imipenem, fosfomycin, and nitrofurantoin had the best effect against isolates of E. coli. PCR assay demonstrated that the frequency of bla, bla, and bla genes among AmpC β-lactamases-producing strains were 39%, 1%, and 17.5%, respectively. This study reports the first detection of fosfomycin resistance in Iran. This study indicated the increasing prevalence of UTI isolates of E. coli-harboring ESBL and AmpC β-lactamases genes in Iran. Therefore, due to the high rate of bla and bla genes and emergence of fosfomycin-resistant E. coli isolates, we recommend continuous monitoring of antibiotic resistance as well as attention to guidelines of infection controls.

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“…Antimicrobial agents can greatly contribute to the clinical control of UTI. Antimicrobial resistance, in particular, multidrug-resistant Gram-negative bacilli, has posed a remarkable challenge for the clinical settings ( Saffar et al., 2016 ). Diverse drug resistance mechanisms have been introduced in Gram-negative bacteria among which, extended-spectrum β-lactamases (ESBL) production, AmpC β-lactamase generation, efflux, and porin deficiency can be mentioned.…”

Section: Introductionmentioning

confidence: 99%

Clonal Relationship and Resistance Profiles Among ESBL-Producing Escherichia coli

Dehkharghani

Haghighat

Farzami

et al. 2021

Front. Cell. Infect. Microbiol.

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AmpC β-lactamases hydrolyze all β-lactams except cefepime and carbapenems. The study of AmpC-producing E. coli has high priority for the infection control committee. This research is aimed to investigate the resistant urinary AmpC-generating E. coli isolates and identify their genetic variety. Some 230 E. coli isolates from patients suffering urinary tract infection symptoms were studied in 2017–2018 to assess their susceptibility toward antimicrobial agents. AmpC gene was evaluated by PCR and molecular typing using the 10-loci MLVA method. MLVA images were examined by BioNumerics 6.6 software through the use of the UPGMA algorithms. Thirty-eight AmpC-generating E. coli isolates were detected. The most abundant determinant was blaCIT and blaEBC, blaFOX, and blaDHA had the next ranks, respectively. Six major clusters and a singleton were identified by MLVA. AmpC beta-lactamases in urinary isolates of E. coli in the hospital under study and high rate of additional resistance to gentamicin, cotrimoxazole and ciprofloxacin. The most frequent gene determinant of AmpC beta-lactamase was blaCIT and vary depending on time and geographical location.

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Prevalence of AmpC β-lactamase in Clinical Isolates of Escherichia coli, Klebsiella spp., and Proteus mirabilis in a Tertiary Hospital in Tehran, Iran

Abstract: Background: AmpC β-lactamase confers resistance to a variety of β-lactam agents, and all plasmid-mediated AmpC genes are con-

Cited by 12 publications

References 13 publications

The prevalence of ESBL and AmpC β-lactamases in uropathogenic isolates of Escherichia coli in a tertiary care hospital in Southwest Iran

The prevalence of ESBL and AmpC β-lactamases in uropathogenic isolates of Escherichia coli in a tertiary care hospital in Southwest Iran

Emergence of fosfomycin resistance among isolates of Escherichia coli harboring extended-spectrum and AmpC β-lactamases

Clonal Relationship and Resistance Profiles Among ESBL-Producing Escherichia coli

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