Prevalence of antibiotic resistance in lactic acid bacteria isolated from the faeces of broiler chicken in Malaysia

Abstract

BackgroundProbiotics are commonly used as feed additive to substitute antibiotic as growth promoter in animal farming. Probiotic consists of lactic acid bacteria (LAB), which enhance the growth and health of the animal. Probiotic also have higher possibility to become pathogenic bacteria that may carry antibiotic resistant gene that can be transmitted to other LAB species. The aim of this study was to identify the LAB species in the faeces of broiler chicken and to determine the prevalence of antibiotic resistant in LAB of broiler chicken.MethodsSixty faeces samples were collected from wet markets located in Klang Valley of Malaysia for the isolation of LAB using de-Mann Rogosa Sharpe medium. Thirteen species of LAB were obtained in this study and the identification of LAB was performed by using API test kit on the basis of carbohydrate fermentation profile. Antibiotic susceptibility assay was then carried out to determine the prevalence of LAB antibiotic resistance.ResultsLactococcus lactis subsp lactis was found in nine out of sixty faecal samples. Lactobacillus paracasei was the second common LAB species isolated from chicken faecal. No significant difference (P > 0.05) was found between the occurrence of Lactobacillus brevis, Lactobacillus curvatus, Lactobacillus plantarum, Leuconostoc lactis mesenteroides subsp mesenteroides/dectranium and Pediococcus pentosaceus isolated from 5 different locations. Most of the isolated LAB was resistant to antibiotic and high variability of the antibiotic resistance was observed among the LAB against 15 types of antibiotics. Penicillin, amoxicillin, chloramphenicol, and ampicillin had significant higher (P< 0.05) inhibitory zone than nalidixic acid, gentamycin, sulphamethoxazole, kanamycin, and streptomycin.ConclusionsMany species of LAB were isolated from the faecal samples of broiler chicken that resistance to the common antibiotics used in the farm. The development of resistant against antibiotics in LAB can be attributed to the long term exposure of antibiotic as growth promoter and therapeutic agents. Thus, it is essential to advise farmer the safety measure of antibiotic application in animal farming. Additionally, the supplementation of probiotic in animal feeding also needs more attention and close monitoring.

Isolation of Lactic Acid Bacteria with Cholesterol-Lowering Activity from Digestive Tracts of Indonesian Native Chickens

“…This lead to accordance with Adams & Nout (2001) who stated that LAB had negative catalase activity. The gas production assay from all isolates showed negative result hence the isolates were homofermentative (Shazali et al, 2014). Based on biochemical point of view, LAB consist of both homofermenters, yielding mainly lactic acid, and heterofermenters, which, apart from lactic acid, produce a large variety of fermentation metabolites such as acetic acid, carbon dioxide, ethanol, and formic acid (Kleerebezem & Hugenholtz, 2003).…”

Impact of exopolysaccharide production on functional properties of some Lactobacillus salivarius strains

“…The dominant prevalence of L. salivarius strains is not that surprising as this strain was reported to be an important member of indigenous microbiota of GI tract of both humans and animals (Raftis et al 2011). Previously several chicken isolate LAB strains including L. salivarius were identified and their potential technological performances were evaluated (Bouzaine et al 2005;Taheri et al 2009;Shazali et al 2014). Importantly the probiotic functions of several L. salivarius strains isolated from chickens including reducing Salmonellae numbers (Zhang et al 2007), antagonistic effect on chicken pathogens (Jin et al 1996), preventing Salmonella enteritidis colonization (Pascual et al 1999) were reported showing the crucial role of L. salivarius strains for poultry industry.…”

Gut microbiota, inflammation and colorectal cancer

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