Prevalence of Bartonella henselae and Bartonella clarridgeiae in cats and dogs in Korea

Kim, You seok; Seo, Kyoung Won; Lee, Jong Hwa; Choi, Eung‐Chil; Lee, Hee Woo; Hwang, Cheol‐Yong; Shin, Namsoo; Youn, Hee Jeong; Youn, Hwa Young

doi:10.4142/jvs.2009.10.1.85

Cited by 49 publications

(50 citation statements)

References 19 publications

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“…This result is similar to those of other studies performed in others parts of the world using similar molecular methods (PCR), including Italy (18%) (Fabbi et al 2004), Holland (22%) (Bergmans et al 1997), France (16.5%) (Gurfield et al 2001) and Thailand (16.3%) (Inoue et al 2009). However, the prevalence described here is lower than that shown in other reports, including reports from South Korea (33.3%) (Kim et al 2009) and Brazil (RJ, 90%) (Souza 2009), both of which used molecular studies similar to ours, as well as reports from the Philippines (61%) (Chomel et al 1999) or even in other regions of Brazil (SP, 46%) (Slhessarenko et al 1996). These other studies were based on serological methods, which were used to detect anti-Bartonella antibodies, thus detecting not only active infection but also any past infection that resulted in antibody production.…”

Section: Discussioncontrasting

confidence: 53%

Prevalence of Bartonella henselae and Bartonella clarridgeiae in cats in the south of Brazil: a molecular study

Staggemeier

Venker

PS____Klein

et al. 2010

Mem. Inst. Oswaldo Cruz

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Section: Discussioncontrasting

confidence: 53%

Prevalence of Bartonella henselae and Bartonella clarridgeiae in cats in the south of Brazil: a molecular study

Staggemeier

Venker

PS____Klein

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…Although some clinical manifestations were associated with natural and experimental infections in cats, most feline Bartonella infections are considered to be subclinical (Kordick et al 1999, Dehio 2004, Breitschwerdt 2008. The molecular prevalence of Bartonella infection in cats varies worldwide, ranging from 0.3% in Spain to 40.4% in South Korea (Chomel et al 2006, Kim et al 2009, Ayllon et al 2012. The most common Bartonella species detected in cats are B. henselae and B. clarridgeiae, and both have been implicated as the cause of cat scratch disease in humans (Boulouis et al 2005).…”

Section: Introductionmentioning

confidence: 97%

Bartonellae in Domestic and Stray Cats from Israel: Comparison of Bacterial Cultures and High-Resolution Melt Real-Time PCR As Diagnostic Methods

Gutiérrez

Morick

Gross

et al. 2013

Vector-Borne and Zoonotic Diseases

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To determine the occurrence of feline bartonellosis in Israel, blood samples were collected from 179 stray and 155 domestic cats from 18 cities or villages in central and northcentral Israel. Samples were screened for Bartonella infection by culture isolation and molecular detection using high-resolution melt (HRM) real-time PCR assay targeting the 16S-23S rRNA internal transcribed spacer (ITS). All positive samples were confirmed by two additional HRM real-time PCR assays targeting two fragments of the β-subunit of RNA polymerase (rpoB) and the 16S rRNA genes. The prevalence of Bartonella spp. infection in the general tested population was 25.1% (84/334). A higher prevalence was detected in the stray (30.7%; 55/179) than the domestic cats (18.7%; 29/155). Bartonella henselae, Bartonella clarridgeiae, and Bartonella koehlerae were highly prevalent in both cat populations, however their distribution among the two populations varied significantly (p=0.016). B. clarridgeiae and B. koehlerae were found to be more prevalent in stray than domestic cats, whereas B. henselae was evenly distributed. Co-infection with two or more different Bartonella spp. was determined in 2.1% (7) of the cats. The ITS HRM real-time PCR assay used in this study was shown to have a greater screening power than bacterial isolation, detecting 94.0% (79/84) compared to 35.7% (30/84), respectively, of all positive samples. The high prevalence of these zoonotic Bartonella species, coupled with the overpopulation of stray cats, and increased numbers of domestic cats in the major urban centers in Israel represent a significant threat for the public health in this country.

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“…Furthermore, Kim et al [14] reported that the results of nested-PCR from saliva (44.1%) were more than the results of nested-PCR from blood (41.8%). In our study, the results of nested-PCR from blood (36%) were higher than the results of nested-PCR from oral swabs (31%).…”

Section: Discussionmentioning

confidence: 99%

Bartonella henselae Infeksiyonunun Saptanmasında Kültür Sonrası PCR, Nested-PCR ve IFA Yöntemlerinin Karşılaştırılması

Sığırcı¹,

Çelik²,

Kahraman³

et al. 2017

Kafkas Univ Vet Fak Derg

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Cats are the main reservoirs of zoonotic Bartonella henselae which are the causative agents of Cat Scratch Disease (CSD). The aim of this study is to compare three diagnostic methods including culture followed by PCR from whole blood, nested-PCR from oral swab and whole blood, and IFA from serum samples. The diagnosis of B. henselae was compared with the bacteriological methods following conventional PCR and by two separate nested PCR from blood, and oral cavity swabs which were collected from 81 pet and stray cats in Istanbul, Turkey. Also the seroprevalence was determined by indirect fluorescent antibody (IFA) technique in the same animals. Bartonella spp. was determined in 26 (32%) of the blood samples by culture. Twenty of them were identified as B. henselae and 6 of them were B. clarridgeiae by following conventional PCR assay. Of 81 whole blood samples subjected to PCR, 29 (36%) were positive in the nested reaction. Of these, 20 were identified as B.henselae and 8 were B. clarridgeiae. However, one of the samples was found to be positive for both B. henselae and B. clarridgeiae DNA by the nested reaction. Of 81oral swab samples subjected to PCR, 25 (31%) were positive in the nested reaction. Of these, 19 were identified as B. henselae and 6 were B. clarridgeiae. B.henselae IgG antibody seroprevalence was detected as 67% (54/81). Using the combination of blood and oral samples by Nested-PCR simultaneously may increase the sensitivity of the test. Also, the combination of the blood culture with nested-PCR and serology is likely to give the most definitive information in the diagnosis of bartonellosis in cats. Keywords

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Prevalence of Bartonella henselae and Bartonella clarridgeiae in cats and dogs in Korea

Cited by 49 publications

References 19 publications

Prevalence of Bartonella henselae and Bartonella clarridgeiae in cats in the south of Brazil: a molecular study

Prevalence of Bartonella henselae and Bartonella clarridgeiae in cats in the south of Brazil: a molecular study

Bartonellae in Domestic and Stray Cats from Israel: Comparison of Bacterial Cultures and High-Resolution Melt Real-Time PCR As Diagnostic Methods

Bartonella henselae Infeksiyonunun Saptanmasında Kültür Sonrası PCR, Nested-PCR ve IFA Yöntemlerinin Karşılaştırılması

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