Prevalence of brucellosis and infectious bovine rhinotracheitis in organized dairy farms in India

Trangadia, B. J.; Rana, Samir Kumar; Mukherjee, Falguni; Srinivasan, V.A.

doi:10.1007/s11250-009-9407-7

Cited by 38 publications

(21 citation statements)

References 9 publications

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“…seroprevalence has been reported in Indian dairy herds [26,27]. Long-term serological studies at national level indicated, 5% cattle infected with brucellosis [28].…”

Section: /5mentioning

confidence: 99%

Assessment of Haemato-biochemical Parameters and Therapeutics on Brucella Infected Cattle

Kushwaha¹

2014

JMEN

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Brucellosis is a contagious systemic bacterial disease of livestock. Antibiotics commonly being used have limited efficacy against the disease. In the present study, therapy with antibiotics on Brucella infected cattle was performed and subsequently haemato-biochemical parameters, detection of anti-Brucella antibodies in both serum and milk by different diagnostics and shedding of Brucella in milk were assessed. Treatment with antibiotics; streptomycin and long acting tetracycline alone and in combination were given for four weeks. Hb, TEC, TLC, neutrophils, eosinophils, lymphocytes, monocytes count, AST, ALT and SD values were lower whereas, PCV, serum glucose, total protein, albumin and creatinine were higher in Brucella infected cattle. Treatment with combination therapy showed promising results in these animals. Haemato-biochemical parameters were become near to normal in most of animals that received combination therapy. However, not all animals were returned to normal after treatment but a significant numbers in combination therapy were reduced anti-Brucella antibody titre, both in serum and milk. The level of shedding of Brucella in milk had also gone undetectable. KeywordsBrucellosis; Cattle; Haemato-biochemical parameters; Oxytetracycline; Streptomycin Reaction (PCR) based assays have been developed for the rapid identification of Brucella. It is advantageous to detect DNA of pathogen that rendered biologically safe and hence, reduced the risk of infection to laboratory workers. In addition, PCR is more sensitive than traditional culture method [8].There is limited evidence on treatment aspect of Brucella infected cattle as well as their haemato-biochemical parameters. Brucella is inaccessible to antibiotics as they are facultative intracellular pathogens. Many antimicrobials are active against Brucella species; however, clinical efficacy does not always correlate with in vitro susceptibility [9]. Treatments for brucellosis have been tried previously but none was found successful [10,11]. Streptomycin acts synergistically with penicillin or tetracycline to inhibit the growth of B. abortus within bovine cells cultured in vitro [12]. Considering the above facts, the present work was intended to determine the efficacy of antibiotic therapy and alteration in the haemato-biochemical parameters if any, in Brucella infected cattle after treatment. Materials and MethodsA total of 27 cattle which showed antibodies both in serum and milk as well as Brucella DNA in milk were selected for the study. ELISA, RBPT and STAT were employed on serum whereas, MRT and ELISA on milk to detect the anti-Brucella antibodies. Milk samples were processed for detection of pathogen itself in form of its DNA (16S rRNA) by PCR [13]. Reference antigens and serum for RBPT, STAT and, for MRT, Abortus Bang Ring Antigen (ABR Antigen) were procured from the Indian Veterinary Research Institute, Izatnagar (India). AniGen B. Brucella Ab ELISA kit (Cat. No. EB43-01) (i-ELISA and Milk-ELISA) was procured

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“…seroprevalence has been reported in Indian dairy herds [26,27]. Long-term serological studies at national level indicated, 5% cattle infected with brucellosis [28].…”

Section: /5mentioning

confidence: 99%

Assessment of Haemato-biochemical Parameters and Therapeutics on Brucella Infected Cattle

Kushwaha¹

2014

JMEN

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“…Higher seroprevalence of 67% was reported by Obando et al (1999) in Venezuela from 615 beef cows and by SolisCalderon et al (2003) in Yucatan, Mexico who sampled 564 animals from 35 beef herds and reported individual seroprevalence of 54%. With regard to other tropical areas worldwide, Trangadia et al (2010) reported 22% ELISA Thompson et al (2006) reported 47% seroprevalence in Brazil from 2,343 cattle from 72 farms and in Zambia, and Mweene et al (2003) reported a seroprevalence of 48% in a small sample size of 116 animals. For comparison, in Belgium, individual and median within-herd seroprevalence was reported to be 36% and 33%, respectively (Boelaert et al 2000), and in England, mean cattle and herd seroprevalence was found to be 42.5% and 43%, respectively (Woodbine et al 2009).…”

Section: Bhv-1 Seroprevalencementioning

confidence: 99%

Seroprevalence of infectious bovine rhinotracheitis and bovine viral diarrhea virus type 1 and type 2 in non-vaccinated cattle herds in the Pacific Region of Central Costa Rica

Raizman

Pogranichniy

Negrón

et al. 2010

Trop Anim Health Prod

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The objectives of this cross-sectional study were to estimate the seroprevalence of infectious bovine rhinotracheitis (IBR, BHV-1) and bovine viral diarrhea virus (BVDV) in a population of non-vaccinated, double purpose, dairy and beef herds in the Pacific Region of Central Costa Rica. Blood samples were collected from a total of 496 animals from 35 herds. Sera were tested for antibodies against BHV-1(IBR) and BVDV types 1 and 2 using serum neutralization test. The average number of animals tested in each herd for each of the viruses was 14. Overall individual seroprevalence was 48%, 27%, and 19% for IBR, BVDV type 1, and BVDV type 2, respectively. Median within-herd seroprevalence for IBR, BVDV type 1 and type 2 were 43%, 27%, and 24%, respectively.

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“…Numerous studies have shown that the MRT can be used to scan a herd of animals, and further demonstrated that such reaction possesses sufficient sensitivity and specificity to diagnose Brucella in a single animal (Trangadia et al, 2010). In other words, bacteria isolation is necessary to determine whether the animal itself is infected with B. melitensis, or it contains only antibodies against such bacteria.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Brucella melitensis strains from Syrian bovine milk samples

Al-Mariri¹

2015

BJVM

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SummaryAl-Mariri, A., 2015. Isolation of Brucella melitensis strains from Syrian bovine milk samples. Bulg. J. Vet. Med., 18, No 1, 4048. Brucellosis is a worldwide zoonotic disease that still constitutes a major public health problem in Syria. In the current study, Brucella has been isolated from cow milk samples and typed by both molecular and conventional techniques. Over a 6-year period (2002)(2003)(2004)(2005)(2006)(2007) 2372 milk samples were collected from Syrian cow herds. The milk ring test (MRT) was performed where 57% of samples were found to be positive. Milk samples were cultured on the Brucella selective medium and incubated for 24-48 h. One-quarter of milk samples were contaminated with Brucella, which is considered as a relatively high rate of contamination. In addition, biochemical properties of isolates have been identified. All isolated Brucella strains were positive for catalase, oxidase and urea reaction, did not need CO 2 to grow and rarely produced H 2 S, could grow in the presence of fuchsin and thionin. The agglutination test was negative with anti-R and positive with anti-M and anti-A sera; so it was determined as B. melitensis strain, biovar 3. A specific PCR assay targeting the bcsp31 gene and the specific integration of IS711 elements within the genome of Brucella species has been used. Molecular typing results were compatible with conventional methods.

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Prevalence of brucellosis and infectious bovine rhinotracheitis in organized dairy farms in India

Cited by 38 publications

References 9 publications

Assessment of Haemato-biochemical Parameters and Therapeutics on Brucella Infected Cattle

Assessment of Haemato-biochemical Parameters and Therapeutics on Brucella Infected Cattle

Seroprevalence of infectious bovine rhinotracheitis and bovine viral diarrhea virus type 1 and type 2 in non-vaccinated cattle herds in the Pacific Region of Central Costa Rica

Isolation of Brucella melitensis strains from Syrian bovine milk samples

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