Prevalence of Coxiella burnetii antibodies in Danish dairy herds

Agger, Jens Frederik; Christoffersen, Anna-Bodil; Rattenborg, Erik; Nielsen, Jørgen S.; Agerholm, Jørgen Steen

doi:10.1186/1751-0147-52-5

Cited by 58 publications

(55 citation statements)

References 5 publications

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“…A correlation was, however, found between the Cq values of the cow samples (weighted according to milk yield) and the C. burnetii concentration in BTM (P = 0.02, Spearman correlation coefficient). Agger et al (2010) found antibodies against C. burnetii in 59% of BTM samples from 100 randomly selected Danish dairy herds. In the present investigation including 12 herds, 25% of the 1,514 individual cow samples were seropositive, whereas 32% were positive for C. burnetii DNA.…”

Section: Analysis Of Btmmentioning

confidence: 94%

“…Twelve herds were recruited among 100 randomly selected Danish dairy herds examined for BMT antibodies against C. burnetii once in the spring 2008 (Agger et al, 2010). The herds consisted of 10 randomly selected herds among the test-positive herds; in addition, 1 BTM test-intermediate herd and 1 BTM test-negative herd (although with ELISA, test-positive cow samples) were selected (Figure 1).…”

Section: Sampling and Antibody Detectionmentioning

confidence: 99%

“…In Denmark, 57% of 742 non-randomly selected cattle herds were found to be antibody positive in bulk tank milk (BTM) samples (Bødker and Christoffersen, 2008), whereas in a more recent study, 59% of 100 randomly selected herds were found to be antibody positive (Agger et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Dynamics of relationship between the presence of Coxiella burnetii DNA, antibodies, and intrinsic variables in cow milk and bulk tank milk from Danish dairy cattle

Angen

Ståhl

Agerholm

et al. 2011

Journal of Dairy Science

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Section: Analysis Of Btmmentioning

confidence: 94%

Section: Sampling and Antibody Detectionmentioning

confidence: 99%

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Dynamics of relationship between the presence of Coxiella burnetii DNA, antibodies, and intrinsic variables in cow milk and bulk tank milk from Danish dairy cattle

Angen

Ståhl

Agerholm

et al. 2011

Journal of Dairy Science

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“…In a study in which milk samples of 44 dairy cattle herds raised in southern Iran were taken from milk tanks and tested using ELISA, Khalili and Sakhaee (2009) detected a positivity rate of 45.4%. On the other hand, Agger et al (2010) reported a positivity rate of 59% in randomly selected cattle herds in Denmark. Based on the ELISA analysis of milk and blood serum samples of 448 cattle from six different herds raised in France, Guatteo et al (2007) detected the presence of anti-C. burnetii antibodies in 264 of the blood serum samples and in 257 of the milk samples.…”

Section: Discussionmentioning

confidence: 97%

Coxiella burnetii in samples from cattle herds and sheep flocks in the Kars region of Turkey

Sağlam¹,

Şahin²

2016

Vet. Med.

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This study was aimed at determining the presence of C. burnetii in cattle and sheep herds in the Kars region of Turkey using serological and molecular methods. As a serological technique, C.burnetii IgG in blood sera and milk samples were investigated with ELISA. The results of these examinations revealed that 108 (43.2%) out of 250 sheep blood serum samples and 52 (14.85%) out of 350 cattle blood serum samples were seropositive for C. burnetii antibodies by ELISA. Out of 350 cattle and 250 sheep milk samples examined with ELISA, 36 (10.28%) and 42 (16.8%) were found to be seropositive, respectively. For molecular analysis, a Trans-PCR amplifying the IS1111A transposase gene of C. burnetii was conducted. Five (1.42%) out of 350 cattle milk samples, and one (0.4%) from 250 sheep milk samples were found to be positive for C. burnetii DNA. The results obtained in this study have demonstrated the presence of Q fever in cattle and sheep in the Kars region, and the dissemination of the infectious agent within milk. This situation poses a potential risk for animal and human health. Ultimately, this study points the way to future investigations into the presence of C. burnetii, which causes Q fever in cattle and sheep, and will contribute to the protection and control of the disease.

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“…This could very well be the result of the presence of compounds of animal origin (such as bovine serum albumin [BSA]) that are commonly used in PCR assays. Since many farm animals can be carriers of C. burnetii (1,4), addition of BSA originating from such animals may explain the contamination of the Master Mix with C. burnetii DNA. An unopened vial of this Master Mix was sent to the St. Antonius Hospital in Nieuwegein (a laboratory that routinely uses a different Master Mix; uses another, nonoverlapping, DNA target in its Q fever PCR; and had experienced no contamination issues).…”

mentioning

confidence: 99%

Contamination of Commercial PCR Master Mix with DNA from Coxiella burnetii

et al. 2010

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Contamination of an in-house diagnostic real-time PCR for Q fever was traced back to a commercially obtained PCR Master Mix. It was established that this Master Mix contained DNA from Coxiella burnetii, probably as a result of the use of compounds of animal origin such as bovine serum albumin.As of 2007 and up to the present time, there has been a large ongoing outbreak of Q fever in Netherlands that is unprecedented both in the number of affected individuals and in its duration (5). Real-time PCR has rapidly been implemented in many diagnostic laboratories in Netherlands as a first-line diagnostic test to identify patients infected with Coxiella burnetii in the acute phase of the disease. In a recent interlaboratory study, it has been shown that multiple PCR approaches based on different combinations of extraction procedures, amplification primers and probes, PCR Master Mixes, and real-time PCR platforms offer equivalent solutions to screening for Q fever (6). In the spring of 2009, the medical microbiology laboratory of the Canisius Wilhelmina Hospital (CWZ) was suddenly confronted with positive PCR results in a no-template control (NTC), indicating contamination of the diagnostic pathway. Analysis of multiple NTCs showed that 10 to 30% yielded a positive PCR result with threshold cycle values such as those found in many clinical samples. The laboratory enforces strict precautionary measures to avoid amplicon or DNA carryover during the entire diagnostic process. Since the contamination coincided with the first use of a new batch of PCR primers and probe, it was suspected that one of these was contaminated off synthesis. A newly ordered batch of primers and probe did not alleviate the problem. Around the same time, the medical microbiology laboratory of the Radboud University Nijmegen Medical Center (RUNMC) experienced similar problems, with important clinical consequences. A patient with a history of a Ross procedure was admitted with fever and probable endocarditis with gradual degradation of the aortic homograft and deterioration of cardiac function despite broad-spectrum antibiotics. All blood cultures were negative, and additional serological tests for culture-negative endocarditis (including Q fever) were performed. During this time, hemodynamic problems occurred and a second cardiac surgery was necessary. The day before surgery, the Q-fever PCR was positive but serological tests (complement fixation test and IgM antibodies) were negative, making chronic Q fever unlikely. It was decided to postpone the cardiac surgery and to repeat the PCR in another laboratory. This PCR was negative, confirming the suspicion of a false-positive PCR result. Fortunately, no serious hemodynamic problems occurred during the time while the operation was deferred. According to protocol and good laboratory practice, numerous measures were undertaken to eliminate any potential source of contamination, but without success.

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Prevalence of Coxiella burnetii antibodies in Danish dairy herds

Cited by 58 publications

References 5 publications

Dynamics of relationship between the presence of Coxiella burnetii DNA, antibodies, and intrinsic variables in cow milk and bulk tank milk from Danish dairy cattle

Dynamics of relationship between the presence of Coxiella burnetii DNA, antibodies, and intrinsic variables in cow milk and bulk tank milk from Danish dairy cattle

Coxiella burnetii in samples from cattle herds and sheep flocks in the Kars region of Turkey

Contamination of Commercial PCR Master Mix with DNA from Coxiella burnetii

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