Prevalence of Encephalitozoon intestinalis and Enterocytozoon bieneusi in Cancer Patients Under Chemotherapy

Abstract

ÖZZorunlu hücre içi parazitler olan mikrosporidia türleri, özellikle immün sistemi baskılanmış hastalarda ciddi enfeksiyonlara yol açan en önemli fırsatçı patojenlerdendir. İnsanı enfekte eden 14 mikrosporidia türü arasında Enterocytozoon bieneusi ve Encephalitozoon intestinalis en yaygın olanlarıdır. Bu çalışmada, kemoterapi alan kanserli hastalarda E.intestinalis ve E.bieneusi prevalansının, immünofloresan antikor ve konvansiyonel boyama yöntemleri ile araştırılması amaçlanmıştır. Çalışmaya, hastanemizin onkoloji ve hematoloji kliniklerinde takip edilen 93 kanserli hasta (58 erkek, 35 kadın) ile kontrol olarak 30 (13 erkek, 17 kadın) sağlıklı gönüllüden alınan toplam 123 dışkı örneği dahil edilmiştir. Hastaların 51 (%55)'inde ishal şikayeti mevcuttur. Tüm örneklerde E.intestinalis ve E.bieneusi varlığı, monoklonal antikorların kullanıldığı ticari bir immünofloresan antikor yöntemi (IFA-MAb; Bordier Affinity Products, İsviçre) ile araştırılmış; ayrıca 50 örnek modifiye trikrom, aside dirençli trikrom ve kalkoflor boyama yöntemleri ile de incelenmiştir. IFA-MAb yöntemi ile hastaların 43 (%46.2)'ünde E.intestinalis, 9 (%9.7)'unda E.bieneusi ve 13 (%14)'ünde karışık enfeksiyon olmak üzere toplam 65 (%69.9) olguda pozitiflik saptanmış; kontrol grubunda ise 2 (%6.7) E.intestinalis, 1 (%3.3) E.bieneusi ve 2 (%6.7) karışık enfeksiyon olmak üzere Geliş Tarihi

Genotyping of Enterocytozoon bieneusi and Subtyping of Blastocystis in Cancer Patients: Relationship to Diarrhea and Assessment of Zoonotic Transmission

“…In the present study, overall infection rates of E. bieneusi and Blastocystis were 1.3% (5/381) and 7.1% (27/381), respectively. Both of them were lower than those in previous reports from cancer patients, with E. bieneusi in Mexico (40%, 4/10), Turkey 23.7% (22/93), and Iran (1.7%, 4/234), and Blastocystis in France (16%, 15/94), Malaysia (21.1%, 43/204), and Turkey (10.8%, 25/232) (Poirier et al, 2011 ; Jiménez-González et al, 2012 ; Kumarasamy et al, 2014 ; Hamamc1 et al, 2015 ; Mirjalali et al, 2015 ; Yersal et al, 2016 ). In fact, infection rates of these two pathogens vary from country to country and within different communities of the same country.…”

The high prevalence of Encephalitozoon intestinalis in patients receiving chemotherapy and children with growth retardation and the validity of real-time PCR in its diagnosis

“…Microsporidian pathogens are opportunistic pathogens and are most commonly found in immunocompromised patients (1,3). They can also cause serious infections in patients receiving chemotherapy and in bone marrow transplant patients and may have an adverse effect on the treatment process (17)(18)(19)(20). In this study, it was found that E. intestinalis was prevalent in patients receiving chemotherapy (40%) and in bone marrow transplant patients admitted with acute gastrointestinal complaints (24%).…”

Prevalence and Genotyping of Microsporidian Parasites in Dogs in Turkey: Zoonotic Concerns

“…Prevalence rates of 6.5% and 10.9% were reported from different parts of the country mainly using the conventional microscopy and staining techniques (Karaman et al 2009;Turk et al 2012). The presence and distribution of E. intestinalis and E. bieneusi in human populations were also confirmed in some studies using serological and molecular diagnostic tools (Cetinkaya et al 2016;Hamamci et al 2015).…”